LOCUS       pJET-Scaf-PJ23119_        3092 bp    DNA     circular UNA 22-MAR-2023
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct.
FEATURES             Location/Qualifiers
     promoter        1..18
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="T7 promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:yz-f3tbldq"">T7 promoter</a>"
                     /label="T7 promoter"
     Scaffold        68..150
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
                     TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
                     /annotation_group="Scaffold: 451 -> 533"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpCas9 Scaffold"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Scaffold
                     (<a href=""urn:local:thjac:2f-c4nnd55"">SpCas9
                     Scaffold</a>)"
                     /label="Scaffold"
     promoter        151..185
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PJ23119"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:1j-cieiaam"">PJ23119</a>"
                     /label="PJ23119"
     primer          complement(589..610)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
     promoter        complement(620..650)
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="14 CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:1h-fz09kes"">14 CmR</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacUV5 (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     rep_origin      complement(977..1565)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     misc_feature    complement(1736..2596)
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="99.77%"
                     /Primary_Match="99.77%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     promoter        complement(2597..2701)
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
ORIGIN      
        1 taatacgact cactataggg agagcggccg ccagatcttc cggatggctc gagtttttca
       61 gcaagatgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
      121 aaaaagtggc accgagtcgg tgcttttttt ttgacagcta gctcagtcct aggtataatg
      181 ctagcatctt tctagaagat ctcctacaat attctcagct gccatggaaa atcgatgttc
      241 ttcttttatt ctctcaagat tttcaggctg tatattaaaa cttatattaa gaactatgct
      301 aaccacctca tcaggaaccg ttgtaggtgg cgtgggtttt cttggcaatc gactctcatg
      361 aaaactacga gctaaatatt caatatgttc ctcttgacca actttattct gcattttttt
      421 tgaacgaggt ttagagcaag cttcaggaaa ctgagacagg aattttatta aaaatttaaa
      481 ttttgaagaa agttcagggt taatagcatc cattttttgc tttgcaagtt cctcagcatt
      541 cttaacaaaa gacgtctctt ttgacatgtt taaagtttaa acctcctgtg tgaaattgtt
      601 atccgctcac aattccacac attatacgag ccggaagcat aaagtgtaaa gcctggggtg
      661 cctaatgagt gagctaactc acattaattg cgttgcgctc actgccaatt gctttccagt
      721 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      781 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      841 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      901 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      961 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1021 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1081 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1141 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1201 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1261 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1321 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1381 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     1441 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1501 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1561 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1621 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1681 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     1741 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1801 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     1861 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     1921 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1981 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     2041 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     2101 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     2161 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2221 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2281 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2341 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2401 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2461 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2521 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2581 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     2641 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     2701 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     2761 cctataaaaa taggcgtatc acgaggccgc ccctgcagcc gaattatatt atttttgcca
     2821 aataattttt aacaaaagct ctgaagtctt cttcatttaa attcttagat gatacttcat
     2881 ctggaaaatt gtcccaatta gtagcatcac gctgtgagta agttctaaac cattttttta
     2941 ttgttgtatt atctctaatc ttactactcg atgagttttc ggtattatct ctatttttaa
     3001 cttggagcag gttccattca ttgttttttt catcatagtg aataaaatca actgctttaa
     3061 cacttgtgcc tgaacaccat atccatccgg cg
//
