LOCUS       pGG-C-dCas9-CDA-UL-D_        8052 bp    DNA     circular UNA 22-MAR-2023
DEFINITION  .
ACCESSION   urn.local...oh-9s73oy9
VERSION     urn.local...oh-9s73oy9
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:88-dgycryy"">C</a>"
                     /label="C"
     CDS             14..4117
                     /product="nickase mutant of the Cas9 endonuclease from the
                      Streptococcus pyogenes Type II CRISPR/Cas system"
                     /note="generates RNA-guided single strand nicks in DNA due
                      to the D10A mutation in the RuvC catalytic domain"
                     /Original_Translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLG
                     NTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVD
                     DSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
                     LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKA
                     ILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSK
                     DTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYD
                     EHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
                     NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRK
                     VTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDIL
                     EDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQ
                     SGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPA
                     IKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKE
                     LGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLK
                     DDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERG
                     GLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVS
                     DFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
                     AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDF
                     ATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPT
                     VAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLII
                     KLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQ
                     KQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHL
                     FTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on protein similarity"
                     /X-Transferred_From="SpCas9(D10A,H840A)"
                     /X-Transferred_Translation_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:9w-dgycryz"">SpCas9(D10A,H840A)</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpCas9-D10A,H840A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:42-dq3hem4"">SpCas9-D10A,H840A</a>"
                     /label="SpCas9-D10A,H840A"
     modified_base   41..43
                     /created_by="mamee"
                     /label="D10A"
     modified_base   2531..2533
                     /created_by="mamee"
                     /label="H840A"
     Linker          4133..4324
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="64-aa linker"
                     /Transferred_Translation_Similarity="95.31%"
                     /Primary_Match="95.31%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:warddecaestecker:30-c8811q7"">64-aa
                     linker</a>"
                     /label="64-aa linker"
     tag             4325..4390
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by
                     an enterokinase cleavage site"
                     /Transferred_Translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="3xFLAG"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:a6-dgycryz"">3xFLAG</a>"
                     /label="3xFLAG"
     tag             4409..4474
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by
                     an enterokinase cleavage site"
                     /Transferred_Translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="3xFLAG"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:a6-dgycryz"">3xFLAG</a>"
                     /label="3xFLAG"
     CDS             4481..5104
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PmCDA1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:z-ciihfgm"">PmCDA1</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:warddecaestecker:2l-c8811q5"">PmCDA1</a>"
                     /label="PmCDA1"
     CDS             5111..5362
                     /created_by="lapau"
                     /label="UGI"
     tag             5366..5374
                     /created_by="lapau"
                     /modified_by="mamee"
                     /label="LVA-tag"
     GG              complement(5378..5388)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:298-ciz70tq"">D</a>"
                     /label="D"
     primer          complement(5408..5428)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        5908..6012
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    6013..6873
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      7044..7632
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          7996..8017
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatggata agaaatactc aataggctta gctatcggca caaatagcgt
       61 cggatgggcg gtgatcactg atgaatataa ggttccgtct aaaaagttca aggttctggg
      121 aaatacagac cgccacagta tcaaaaaaaa tcttataggg gctcttttat ttgacagtgg
      181 agagacagcg gaagcgactc gtctcaaacg gacagctcgt agaaggtata cacgtcggaa
      241 gaatcgtatt tgttatctac aggagatttt ttcaaatgag atggcgaaag tagatgatag
      301 tttctttcat cgacttgaag agtctttttt ggtggaagaa gacaagaagc atgaacgtca
      361 tcctattttt ggaaatatag tagatgaagt tgcttatcat gagaaatatc caactatcta
      421 tcatctgcga aaaaaattgg tagattctac tgataaagcg gatttgcgct taatctattt
      481 ggccttagcg catatgatta agtttcgtgg tcattttttg attgagggag atttaaatcc
      541 tgataatagt gatgtggaca aactatttat ccagttggta caaacctaca atcaattatt
      601 tgaagaaaac cctattaacg caagtggagt agatgctaaa gcgattcttt ctgcacgatt
      661 gagtaaatca agacgattag aaaatctcat tgctcagctc cccggtgaga agaaaaatgg
      721 cttatttggg aatctcattg ctttgtcatt gggtttgacc cctaatttta aatcaaattt
      781 tgatttggca gaagatgcta aattacagct ttcaaaagat acttacgatg atgatttaga
      841 taatttattg gcgcaaattg gagatcaata tgctgatttg tttttggcag ctaagaattt
      901 atcagatgct attttacttt cagatatcct aagagtaaat actgaaataa ctaaggctcc
      961 cctatcagct tcaatgatta aacgctacga tgaacatcat caagacttga ctcttttaaa
     1021 agctttagtt cgacaacaac ttccagaaaa gtataaagaa atcttttttg atcaatcaaa
     1081 aaacggatat gcaggttata ttgatggggg agctagccaa gaagaatttt ataaatttat
     1141 caaaccaatt ttagaaaaaa tggatggtac tgaggaatta ttggtgaaac taaatcgtga
     1201 agatttgctg cgcaagcaac ggacctttga caacggctct attccccatc aaattcactt
     1261 gggtgagctg catgctattt tgagaagaca agaagacttt tatccatttt taaaagacaa
     1321 tcgtgagaag attgaaaaaa tcttgacttt tcgaattcct tattatgttg gtccattggc
     1381 gcgtggcaat agtcgttttg catggatgac tcggaagtct gaagaaacaa ttaccccatg
     1441 gaattttgaa gaagttgtcg ataaaggtgc ttcagctcaa tcatttattg aacgcatgac
     1501 aaactttgat aaaaatcttc caaatgaaaa agtactacca aaacatagtt tgctttatga
     1561 gtattttacg gtttataacg aattgacaaa ggtcaaatat gttactgaag gaatgcgaaa
     1621 accagcattt ctttcaggtg aacagaagaa agccattgtt gatttactct tcaaaacaaa
     1681 tcgaaaagta accgttaagc aattaaaaga agattatttc aaaaaaatag aatgttttga
     1741 tagtgttgaa atttcaggag ttgaagatag atttaatgct tcattaggta cctaccatga
     1801 tttgctaaaa attattaaag ataaagattt tttggataat gaagaaaatg aagatatctt
     1861 agaggatatt gttttaacat tgaccttatt tgaagatagg gagatgattg aggaaagact
     1921 taaaacatat gctcacctct ttgatgataa ggtgatgaaa cagcttaaac gtcgccgtta
     1981 tactggttgg ggacgtttgt ctcgaaaatt gattaatggt attagggata agcaatctgg
     2041 caaaacaata ttagattttt tgaaatcaga tggttttgcc aatcgcaatt ttatgcagct
     2101 gatccatgat gatagtttga catttaaaga agacattcaa aaagcacaag tgtctggaca
     2161 aggcgatagt ttacatgaac atattgcaaa tttagctggt agccctgcta ttaaaaaagg
     2221 tattttacag actgtaaaag ttgttgatga attggtcaaa gtaatggggc ggcataagcc
     2281 agaaaatatc gttattgaaa tggcacgtga aaatcagaca actcaaaagg gccagaaaaa
     2341 ttcgcgagag cgtatgaaac gaatcgaaga aggtatcaaa gaattaggaa gtcagattct
     2401 taaagagcat cctgttgaaa atactcaatt gcaaaatgaa aagctctatc tctattatct
     2461 ccaaaatgga agagacatgt atgtggacca agaattagat attaatcgtt taagtgatta
     2521 tgatgtcgat gccattgttc cacaaagttt ccttaaagac gattcaatag acaataaggt
     2581 cttaacgcgt tctgataaaa atcgtggtaa atcggataac gttccaagtg aagaagtagt
     2641 caaaaagatg aaaaactatt ggagacaact tctaaacgcc aagttaatca ctcaacgtaa
     2701 gtttgataat ttaacgaaag ctgaacgtgg aggtttgagt gaacttgata aagctggttt
     2761 tatcaaacgc caattggttg aaactcgcca aatcactaag catgtggcac aaattttgga
     2821 tagtcgcatg aatactaaat acgatgaaaa tgataaactt attcgagagg ttaaagtgat
     2881 taccttaaaa tctaaattag tttctgactt ccgaaaagat ttccaattct ataaagtacg
     2941 tgagattaac aattaccatc atgcccatga tgcgtatcta aatgccgtcg ttggaactgc
     3001 tttgattaag aaatatccaa aacttgaatc ggagtttgtc tatggtgatt ataaagttta
     3061 tgatgttcgt aaaatgattg ctaagtctga gcaagaaata ggcaaagcaa ccgcaaaata
     3121 tttcttttac tctaatatca tgaacttctt caaaacagaa attacacttg caaatggaga
     3181 gattcgcaaa cgccctctaa tcgaaactaa tggggaaact ggagaaattg tctgggataa
     3241 agggcgagat tttgccacag tgcgcaaagt attgtccatg ccccaagtca atattgtcaa
     3301 gaaaacagaa gtacagacag gcggattctc caaggagtca attttaccaa aaagaaattc
     3361 ggacaagctt attgctcgta aaaaagactg ggatccaaaa aaatatggtg gttttgatag
     3421 tccaacggta gcttattcag tcctagtggt tgctaaggtg gaaaaaggga aatcgaagaa
     3481 gttaaaatcc gttaaagagt tactagggat cacaattatg gaaagaagtt cctttgaaaa
     3541 aaatccgatt gactttttag aagctaaagg atataaggaa gttaaaaaag acttaatcat
     3601 taaactacct aaatatagtc tttttgagtt agaaaacggt cgtaaacgga tgctggctag
     3661 tgccggagaa ttacaaaaag gaaatgagct ggctctgcca agcaaatatg tgaatttttt
     3721 atatttagct agtcattatg aaaagttgaa gggtagtcca gaagataacg aacaaaaaca
     3781 attgtttgtg gagcagcata agcattattt agatgagatt attgagcaaa tcagtgaatt
     3841 ttctaagcgt gttattttag cagatgccaa tttagataaa gttcttagtg catataacaa
     3901 acatagagac aaaccaatac gtgaacaagc agaaaatatt attcatttat ttacgttgac
     3961 gaatcttgga gctcccgctg cttttaaata ttttgataca acaattgatc gtaaacgata
     4021 tacgtctaca aaagaagttt tagatgccac tcttatccat caatccatca ctggtcttta
     4081 tgaaacacgc attgatttga gtcagctagg aggtgacggt ggaggaggtt ctggaggtgg
     4141 aggttctgct gagtatgtgc gagccctctt tgactttaat gggaatgatg aagaggatct
     4201 tccctttaag aaaggagaca tcctgagaat ccgggataag cctgaggagc agtggtggaa
     4261 tgcagaggac agcgaaggaa agagggggat gattcctgtc ccttacgtgg agaagtattc
     4321 cggagactat aaggaccacg acggagacta caaggatcat gatattgatt acaaagacga
     4381 tgacgataag tctaggctcg agtccggaga ctataaggac cacgacggag actacaagga
     4441 tcatgatatt gattacaaag acgatgacga taagtctagg atgaccgacg ctgagtacgt
     4501 gagaatccat gagaagttgg acatctacac gtttaagaaa cagtttttca acaacaaaaa
     4561 atccgtgtcg catagatgct acgttctctt tgaattaaaa cgacggggtg aacgtagagc
     4621 gtgtttttgg ggctatgctg tgaataaacc acagagcggg acagaacgtg gcattcacgc
     4681 cgaaatcttt agcattagaa aagtcgaaga atacctgcgc gacaaccccg gacaattcac
     4741 gataaattgg tactcatcct ggagtccttg tgcagattgc gctgaaaaga tcttagaatg
     4801 gtataaccag gagctgcggg ggaacggcca cactttgaaa atctgggctt gcaaactcta
     4861 ttacgagaaa aatgcgagga atcaaattgg gctgtggaat ctcagagata acggggttgg
     4921 gttgaatgta atggtaagtg aacactacca atgttgcagg aaaatattca tccaatcgtc
     4981 gcacaatcaa ttgaatgaga atagatggct tgagaagact ttgaagcgag ctgaaaaacg
     5041 acggagcgag ttgtccatta tgattcaggt aaaaatactc cacaccacta agagtcctgc
     5101 tgtttctaga atgaccaacc tttccgacat catagagaag gaaacaggca aacagttggt
     5161 catccaagag tcgatactca tgcttcctga agaagttgag gaggtcattg ggaataagcc
     5221 ggaaagtgac attctcgtac acactgcgta tgatgagagc accgatgaga acgtgatgct
     5281 gctcacgtca gatgccccag agtacaaacc ctgggctctg gtgattcagg actctaatgg
     5341 agagaacaag atcaagatgc taggactggt tgcataatca gtgagaccga attctcgccc
     5401 tatagtgagt cgtattacct ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     5461 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     5521 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     5581 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     5641 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     5701 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     5761 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     5821 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     5881 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5941 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6001 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     6061 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     6121 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6181 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6241 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6301 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6361 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6421 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6481 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6541 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6601 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6661 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6721 gagcgtggct ctcgaggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6781 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6841 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6901 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6961 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7021 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7081 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7141 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     7201 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7261 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7321 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7381 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7441 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7501 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7561 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7621 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7681 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7741 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7801 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     7861 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     7921 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     7981 atgttgtgtg gaattgtgag cggataacaa tttcacacga gctatttagg tgacactata
     8041 gaagtgaagc tt
//
