LOCUS pGG-C-SpCas9-D 6795 bp DNA circular UNA 22-MAR-2023 DEFINITION . ACCESSION urn.local...c-co498nk VERSION urn.local...c-co498nk KEYWORDS . SOURCE Streptococcus pyogenes ORGANISM Streptococcus pyogenes. FEATURES Location/Qualifiers GG 1..11 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="C" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; C" /label="C" CDS 14..4120 /created_by="lapau" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpCas9" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpCas9" /label="SpCas9" GG complement(4121..4131) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="D" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; D" /label="D" primer complement(4151..4171) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)" /label="GG_SEQ_rev" promoter 4651..4755 /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)" /label="amp" misc_feature 4756..5616 /X-Transferred_From="AmpR" /X-Transferred_Similarity="100.00%" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR" /label="AmpR" /note="Geneious type: Bacterial Selection" rep_origin 5787..6375 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 6739..6760 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)" /label="GG_SEQ_fw" ORIGIN 1 ggtctcaggc tccatggata agaaatactc aataggctta gatatcggca caaatagcgt 61 cggatgggcg gtgatcactg atgaatataa ggttccgtct aaaaagttca aggttctggg 121 aaatacagac cgccacagta tcaaaaaaaa tcttataggg gctcttttat ttgacagtgg 181 agagacagcg gaagcgactc gtctcaaacg gacagctcgt agaaggtata cacgtcggaa 241 gaatcgtatt tgttatctac aggagatttt ttcaaatgag atggcgaaag tagatgatag 301 tttctttcat cgacttgaag agtctttttt ggtggaagaa gacaagaagc atgaacgtca 361 tcctattttt ggaaatatag tagatgaagt tgcttatcat gagaaatatc caactatcta 421 tcatctgcga aaaaaattgg tagattctac tgataaagcg gatttgcgct taatctattt 481 ggccttagcg catatgatta agtttcgtgg tcattttttg attgagggag atttaaatcc 541 tgataatagt gatgtggaca aactatttat ccagttggta caaacctaca atcaattatt 601 tgaagaaaac cctattaacg caagtggagt agatgctaaa gcgattcttt ctgcacgatt 661 gagtaaatca agacgattag aaaatctcat tgctcagctc cccggtgaga agaaaaatgg 721 cttatttggg aatctcattg ctttgtcatt gggtttgacc cctaatttta aatcaaattt 781 tgatttggca gaagatgcta aattacagct ttcaaaagat acttacgatg atgatttaga 841 taatttattg gcgcaaattg gagatcaata tgctgatttg tttttggcag ctaagaattt 901 atcagatgct attttacttt cagatatcct aagagtaaat actgaaataa ctaaggctcc 961 cctatcagct tcaatgatta aacgctacga tgaacatcat caagacttga ctcttttaaa 1021 agctttagtt cgacaacaac ttccagaaaa gtataaagaa atcttttttg atcaatcaaa 1081 aaacggatat gcaggttata ttgatggggg agctagccaa gaagaatttt ataaatttat 1141 caaaccaatt ttagaaaaaa tggatggtac tgaggaatta ttggtgaaac taaatcgtga 1201 agatttgctg cgcaagcaac ggacctttga caacggctct attccccatc aaattcactt 1261 gggtgagctg catgctattt tgagaagaca agaagacttt tatccatttt taaaagacaa 1321 tcgtgagaag attgaaaaaa tcttgacttt tcgaattcct tattatgttg gtccattggc 1381 gcgtggcaat agtcgttttg catggatgac tcggaagtct gaagaaacaa ttaccccatg 1441 gaattttgaa gaagttgtcg ataaaggtgc ttcagctcaa tcatttattg aacgcatgac 1501 aaactttgat aaaaatcttc caaatgaaaa agtactacca aaacatagtt tgctttatga 1561 gtattttacg gtttataacg aattgacaaa ggtcaaatat gttactgaag gaatgcgaaa 1621 accagcattt ctttcaggtg aacagaagaa agccattgtt gatttactct tcaaaacaaa 1681 tcgaaaagta accgttaagc aattaaaaga agattatttc aaaaaaatag aatgttttga 1741 tagtgttgaa atttcaggag ttgaagatag atttaatgct tcattaggta cctaccatga 1801 tttgctaaaa attattaaag ataaagattt tttggataat gaagaaaatg aagatatctt 1861 agaggatatt gttttaacat tgaccttatt tgaagatagg gagatgattg aggaaagact 1921 taaaacatat gctcacctct ttgatgataa ggtgatgaaa cagcttaaac gtcgccgtta 1981 tactggttgg ggacgtttgt ctcgaaaatt gattaatggt attagggata agcaatctgg 2041 caaaacaata ttagattttt tgaaatcaga tggttttgcc aatcgcaatt ttatgcagct 2101 gatccatgat gatagtttga catttaaaga agacattcaa aaagcacaag tgtctggaca 2161 aggcgatagt ttacatgaac atattgcaaa tttagctggt agccctgcta ttaaaaaagg 2221 tattttacag actgtaaaag ttgttgatga attggtcaaa gtaatggggc ggcataagcc 2281 agaaaatatc gttattgaaa tggcacgtga aaatcagaca actcaaaagg gccagaaaaa 2341 ttcgcgagag cgtatgaaac gaatcgaaga aggtatcaaa gaattaggaa gtcagattct 2401 taaagagcat cctgttgaaa atactcaatt gcaaaatgaa aagctctatc tctattatct 2461 ccaaaatgga agagacatgt atgtggacca agaattagat attaatcgtt taagtgatta 2521 tgatgtcgat cacattgttc cacaaagttt ccttaaagac gattcaatag acaataaggt 2581 cttaacgcgt tctgataaaa atcgtggtaa atcggataac gttccaagtg aagaagtagt 2641 caaaaagatg aaaaactatt ggagacaact tctaaacgcc aagttaatca ctcaacgtaa 2701 gtttgataat ttaacgaaag ctgaacgtgg aggtttgagt gaacttgata aagctggttt 2761 tatcaaacgc caattggttg aaactcgcca aatcactaag catgtggcac aaattttgga 2821 tagtcgcatg aatactaaat acgatgaaaa tgataaactt attcgagagg ttaaagtgat 2881 taccttaaaa tctaaattag tttctgactt ccgaaaagat ttccaattct ataaagtacg 2941 tgagattaac aattaccatc atgcccatga tgcgtatcta aatgccgtcg ttggaactgc 3001 tttgattaag aaatatccaa aacttgaatc ggagtttgtc tatggtgatt ataaagttta 3061 tgatgttcgt aaaatgattg ctaagtctga gcaagaaata ggcaaagcaa ccgcaaaata 3121 tttcttttac tctaatatca tgaacttctt caaaacagaa attacacttg caaatggaga 3181 gattcgcaaa cgccctctaa tcgaaactaa tggggaaact ggagaaattg tctgggataa 3241 agggcgagat tttgccacag tgcgcaaagt attgtccatg ccccaagtca atattgtcaa 3301 gaaaacagaa gtacagacag gcggattctc caaggagtca attttaccaa aaagaaattc 3361 ggacaagctt attgctcgta aaaaagactg ggatccaaaa aaatatggtg gttttgatag 3421 tccaacggta gcttattcag tcctagtggt tgctaaggtg gaaaaaggga aatcgaagaa 3481 gttaaaatcc gttaaagagt tactagggat cacaattatg gaaagaagtt cctttgaaaa 3541 aaatccgatt gactttttag aagctaaagg atataaggaa gttaaaaaag acttaatcat 3601 taaactacct aaatatagtc tttttgagtt agaaaacggt cgtaaacgga tgctggctag 3661 tgccggagaa ttacaaaaag gaaatgagct ggctctgcca agcaaatatg tgaatttttt 3721 atatttagct agtcattatg aaaagttgaa gggtagtcca gaagataacg aacaaaaaca 3781 attgtttgtg gagcagcata agcattattt agatgagatt attgagcaaa tcagtgaatt 3841 ttctaagcgt gttattttag cagatgccaa tttagataaa gttcttagtg catataacaa 3901 acatagagac aaaccaatac gtgaacaagc agaaaatatt attcatttat ttacgttgac 3961 gaatcttgga gctcccgctg cttttaaata ttttgataca acaattgatc gtaaacgata 4021 tacgtctaca aaagaagttt tagatgccac tcttatccat caatccatca ctggtcttta 4081 tgaaacacgc attgatttga gtcagctagg aggtgactga tcagtgagac cgaattctcg 4141 ccctatagtg agtcgtatta cctggccgtc gttttacaac gtcgtgactg ggaaaaccct 4201 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc 4261 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc 4321 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact 4381 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc 4441 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc 4501 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga 4561 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag 4621 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 4681 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 4741 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 4801 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 4861 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 4921 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 4981 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 5041 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 5101 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 5161 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 5221 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 5281 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 5341 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 5401 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 5461 ggtgagcgtg gctctcgagg tatcattgca gcactggggc cagatggtaa gccctcccgt 5521 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 5581 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 5641 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 5701 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 5761 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 5821 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 5881 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 5941 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 6001 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 6061 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 6121 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 6181 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 6241 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 6301 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 6361 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 6421 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 6481 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 6541 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 6601 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 6661 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct 6721 cgtatgttgt gtggaattgt gagcggataa caatttcaca cgagctattt aggtgacact 6781 atagaagtga agctt //