LOCUS pGG-C-SpCas9-D 6795 bp DNA circular UNA 22-MAR-2023
DEFINITION .
ACCESSION urn.local...c-co498nk
VERSION urn.local...c-co498nk
KEYWORDS .
SOURCE Streptococcus pyogenes
ORGANISM Streptococcus pyogenes.
FEATURES Location/Qualifiers
GG 1..11
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="C"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; C"
/label="C"
CDS 14..4120
/created_by="lapau"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="SpCas9"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpCas9"
/label="SpCas9"
GG complement(4121..4131)
/created_by="thjac"
/modified_by="thjac"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="D"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; D"
/label="D"
primer complement(4151..4171)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
/label="GG_SEQ_rev"
promoter 4651..4755
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)"
/label="amp"
misc_feature 4756..5616
/X-Transferred_From="AmpR"
/X-Transferred_Similarity="100.00%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR"
/label="AmpR"
/note="Geneious type: Bacterial Selection"
rep_origin 5787..6375
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 6739..6760
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
/label="GG_SEQ_fw"
ORIGIN
1 ggtctcaggc tccatggata agaaatactc aataggctta gatatcggca caaatagcgt
61 cggatgggcg gtgatcactg atgaatataa ggttccgtct aaaaagttca aggttctggg
121 aaatacagac cgccacagta tcaaaaaaaa tcttataggg gctcttttat ttgacagtgg
181 agagacagcg gaagcgactc gtctcaaacg gacagctcgt agaaggtata cacgtcggaa
241 gaatcgtatt tgttatctac aggagatttt ttcaaatgag atggcgaaag tagatgatag
301 tttctttcat cgacttgaag agtctttttt ggtggaagaa gacaagaagc atgaacgtca
361 tcctattttt ggaaatatag tagatgaagt tgcttatcat gagaaatatc caactatcta
421 tcatctgcga aaaaaattgg tagattctac tgataaagcg gatttgcgct taatctattt
481 ggccttagcg catatgatta agtttcgtgg tcattttttg attgagggag atttaaatcc
541 tgataatagt gatgtggaca aactatttat ccagttggta caaacctaca atcaattatt
601 tgaagaaaac cctattaacg caagtggagt agatgctaaa gcgattcttt ctgcacgatt
661 gagtaaatca agacgattag aaaatctcat tgctcagctc cccggtgaga agaaaaatgg
721 cttatttggg aatctcattg ctttgtcatt gggtttgacc cctaatttta aatcaaattt
781 tgatttggca gaagatgcta aattacagct ttcaaaagat acttacgatg atgatttaga
841 taatttattg gcgcaaattg gagatcaata tgctgatttg tttttggcag ctaagaattt
901 atcagatgct attttacttt cagatatcct aagagtaaat actgaaataa ctaaggctcc
961 cctatcagct tcaatgatta aacgctacga tgaacatcat caagacttga ctcttttaaa
1021 agctttagtt cgacaacaac ttccagaaaa gtataaagaa atcttttttg atcaatcaaa
1081 aaacggatat gcaggttata ttgatggggg agctagccaa gaagaatttt ataaatttat
1141 caaaccaatt ttagaaaaaa tggatggtac tgaggaatta ttggtgaaac taaatcgtga
1201 agatttgctg cgcaagcaac ggacctttga caacggctct attccccatc aaattcactt
1261 gggtgagctg catgctattt tgagaagaca agaagacttt tatccatttt taaaagacaa
1321 tcgtgagaag attgaaaaaa tcttgacttt tcgaattcct tattatgttg gtccattggc
1381 gcgtggcaat agtcgttttg catggatgac tcggaagtct gaagaaacaa ttaccccatg
1441 gaattttgaa gaagttgtcg ataaaggtgc ttcagctcaa tcatttattg aacgcatgac
1501 aaactttgat aaaaatcttc caaatgaaaa agtactacca aaacatagtt tgctttatga
1561 gtattttacg gtttataacg aattgacaaa ggtcaaatat gttactgaag gaatgcgaaa
1621 accagcattt ctttcaggtg aacagaagaa agccattgtt gatttactct tcaaaacaaa
1681 tcgaaaagta accgttaagc aattaaaaga agattatttc aaaaaaatag aatgttttga
1741 tagtgttgaa atttcaggag ttgaagatag atttaatgct tcattaggta cctaccatga
1801 tttgctaaaa attattaaag ataaagattt tttggataat gaagaaaatg aagatatctt
1861 agaggatatt gttttaacat tgaccttatt tgaagatagg gagatgattg aggaaagact
1921 taaaacatat gctcacctct ttgatgataa ggtgatgaaa cagcttaaac gtcgccgtta
1981 tactggttgg ggacgtttgt ctcgaaaatt gattaatggt attagggata agcaatctgg
2041 caaaacaata ttagattttt tgaaatcaga tggttttgcc aatcgcaatt ttatgcagct
2101 gatccatgat gatagtttga catttaaaga agacattcaa aaagcacaag tgtctggaca
2161 aggcgatagt ttacatgaac atattgcaaa tttagctggt agccctgcta ttaaaaaagg
2221 tattttacag actgtaaaag ttgttgatga attggtcaaa gtaatggggc ggcataagcc
2281 agaaaatatc gttattgaaa tggcacgtga aaatcagaca actcaaaagg gccagaaaaa
2341 ttcgcgagag cgtatgaaac gaatcgaaga aggtatcaaa gaattaggaa gtcagattct
2401 taaagagcat cctgttgaaa atactcaatt gcaaaatgaa aagctctatc tctattatct
2461 ccaaaatgga agagacatgt atgtggacca agaattagat attaatcgtt taagtgatta
2521 tgatgtcgat cacattgttc cacaaagttt ccttaaagac gattcaatag acaataaggt
2581 cttaacgcgt tctgataaaa atcgtggtaa atcggataac gttccaagtg aagaagtagt
2641 caaaaagatg aaaaactatt ggagacaact tctaaacgcc aagttaatca ctcaacgtaa
2701 gtttgataat ttaacgaaag ctgaacgtgg aggtttgagt gaacttgata aagctggttt
2761 tatcaaacgc caattggttg aaactcgcca aatcactaag catgtggcac aaattttgga
2821 tagtcgcatg aatactaaat acgatgaaaa tgataaactt attcgagagg ttaaagtgat
2881 taccttaaaa tctaaattag tttctgactt ccgaaaagat ttccaattct ataaagtacg
2941 tgagattaac aattaccatc atgcccatga tgcgtatcta aatgccgtcg ttggaactgc
3001 tttgattaag aaatatccaa aacttgaatc ggagtttgtc tatggtgatt ataaagttta
3061 tgatgttcgt aaaatgattg ctaagtctga gcaagaaata ggcaaagcaa ccgcaaaata
3121 tttcttttac tctaatatca tgaacttctt caaaacagaa attacacttg caaatggaga
3181 gattcgcaaa cgccctctaa tcgaaactaa tggggaaact ggagaaattg tctgggataa
3241 agggcgagat tttgccacag tgcgcaaagt attgtccatg ccccaagtca atattgtcaa
3301 gaaaacagaa gtacagacag gcggattctc caaggagtca attttaccaa aaagaaattc
3361 ggacaagctt attgctcgta aaaaagactg ggatccaaaa aaatatggtg gttttgatag
3421 tccaacggta gcttattcag tcctagtggt tgctaaggtg gaaaaaggga aatcgaagaa
3481 gttaaaatcc gttaaagagt tactagggat cacaattatg gaaagaagtt cctttgaaaa
3541 aaatccgatt gactttttag aagctaaagg atataaggaa gttaaaaaag acttaatcat
3601 taaactacct aaatatagtc tttttgagtt agaaaacggt cgtaaacgga tgctggctag
3661 tgccggagaa ttacaaaaag gaaatgagct ggctctgcca agcaaatatg tgaatttttt
3721 atatttagct agtcattatg aaaagttgaa gggtagtcca gaagataacg aacaaaaaca
3781 attgtttgtg gagcagcata agcattattt agatgagatt attgagcaaa tcagtgaatt
3841 ttctaagcgt gttattttag cagatgccaa tttagataaa gttcttagtg catataacaa
3901 acatagagac aaaccaatac gtgaacaagc agaaaatatt attcatttat ttacgttgac
3961 gaatcttgga gctcccgctg cttttaaata ttttgataca acaattgatc gtaaacgata
4021 tacgtctaca aaagaagttt tagatgccac tcttatccat caatccatca ctggtcttta
4081 tgaaacacgc attgatttga gtcagctagg aggtgactga tcagtgagac cgaattctcg
4141 ccctatagtg agtcgtatta cctggccgtc gttttacaac gtcgtgactg ggaaaaccct
4201 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
4261 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
4321 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
4381 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
4441 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
4501 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
4561 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
4621 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
4681 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
4741 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
4801 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
4861 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
4921 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
4981 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
5041 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
5101 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
5161 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
5221 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
5281 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
5341 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
5401 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
5461 ggtgagcgtg gctctcgagg tatcattgca gcactggggc cagatggtaa gccctcccgt
5521 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
5581 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
5641 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
5701 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
5761 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
5821 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
5881 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
5941 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
6001 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
6061 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
6121 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
6181 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
6241 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
6301 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
6361 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
6421 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
6481 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
6541 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
6601 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
6661 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
6721 cgtatgttgt gtggaattgt gagcggataa caatttcaca cgagctattt aggtgacact
6781 atagaagtga agctt
//