LOCUS       pGG-D-GUS-E             4500 bp    DNA     circular SYN 23-FEB-2023
DEFINITION  .
ACCESSION   KF718997
VERSION     KF718997.1
KEYWORDS    .
SOURCE      Cloning vector pGGC051 (unknown)
  ORGANISM  Cloning vector pGGC051
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:89-dgycryy"">D</a>"
                     /label="D"
     CDS             14..1822
                     /gene="gus"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="AHE38521.1"
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
                     /modified_by="mamee"
     GG              complement(1826..1836)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="E"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:82-dgycryx"">E</a>"
                     /label="E"
     primer          complement(1856..1876)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        2356..2460
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    2461..3321
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3492..4080
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4444..4465
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcatca gctatggtcc gtcctgtaga aaccccaacc cgtgaaatca aaaaactcga
       61 cggcctgtgg gcattcagtc tggatcgcga aaactgtgga attgatcagc gttggtggga
      121 aagcgcgtta caagaaagcc gggcaattgc tgtgccaggc agttttaacg atcagttcgc
      181 cgatgcagat attcgtaatt atgcgggcaa cgtctggtat cagcgcgaag tctttatacc
      241 gaaaggttgg gcaggccagc gtatcgtgct gcgtttcgat gcggtcactc attacggcaa
      301 agtgtgggtc aataatcagg aagtgatgga gcatcagggc ggctatacgc catttgaagc
      361 cgatgtcacg ccgtatgtta ttgccgggaa aagtgtacgt atcaccgttt gtgtgaacaa
      421 cgaactgaac tggcagacta tcccgccggg aatggtgatt accgacgaaa acggcaagaa
      481 aaagcagtct tacttccatg atttctttaa ctatgccgga atccatcgca gcgtaatgct
      541 ctacaccacg ccgaacacct gggtggacga tatcaccgtg gtgacgcatg tcgcgcaaga
      601 ctgtaaccac gcgtctgttg actggcaggt ggtggccaat ggtgatgtca gcgttgaact
      661 gcgtgatgcg gatcaacagg tggttgcaac tggacaaggc actagcggga ctttgcaagt
      721 ggtgaatccg cacctctggc aaccgggtga aggttatctc tatgaactgt gcgtcacagc
      781 caaaagccag acagagtgtg atatctaccc gcttcgcgtc ggcatccggt cagtggcagt
      841 gaagggcgaa cagttcctga ttaaccacaa accgttctac tttactggct ttggtcgtca
      901 tgaagatgcg gacttgcgtg gcaaaggatt cgataacgtg ctgatggtgc acgaccacgc
      961 attaatggac tggattgggg ccaactccta ccgtacctcg cattaccctt acgctgaaga
     1021 gatgctcgac tgggcagatg aacatggcat cgtggtgatt gatgaaactg ctgctgtcgg
     1081 ctttaacctc tctttaggca ttggtttcga agcgggcaac aagccgaaag aactgtacag
     1141 cgaagaggca gtcaacgggg aaactcagca agcgcactta caggcgatta aagagctgat
     1201 agcgcgtgac aaaaaccacc caagcgtggt gatgtggagt attgccaacg aaccggatac
     1261 ccgtccgcaa ggtgcacggg aatatttcgc gccactggcg gaagcaacgc gtaaactcga
     1321 cccgacgcgt ccgatcacct gcgtcaatgt aatgttctgc gacgctcaca ccgataccat
     1381 cagcgatctc tttgatgtgc tgtgcctgaa ccgttattac ggatggtatg tccaaagcgg
     1441 cgatttggaa acggcagaga aggtactgga aaaagaactt ctggcctggc aggagaaact
     1501 gcatcagccg attatcatca ccgaatacgg cgtggatacg ttagccgggc tgcactcaat
     1561 gtacaccgac atgtggagtg aagagtatca gtgtgcatgg ctggatatgt atcaccgcgt
     1621 ctttgatcgc gtcagcgccg tcgtcggtga acaggtatgg aatttcgccg attttgcgac
     1681 ctcgcaaggc atattgcgcg ttggcggtaa caagaaaggg atcttcactc gcgaccgcaa
     1741 accgaagtcg gcggcttttc tgctgcaaaa acgctggact ggcatgaact tcggtgaaaa
     1801 accgcagcag ggaggcaaac aataactgct gagaccgaat tctcgcccta tagtgagtcg
     1861 tattacctgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     1921 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     1981 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     2041 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     2101 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     2161 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     2221 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     2281 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     2341 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     2401 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2461 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     2521 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     2581 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     2641 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     2701 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     2761 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     2821 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     2881 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     2941 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     3001 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     3061 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     3121 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggctct
     3181 cgaggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     3241 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     3301 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     3361 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     3421 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3481 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3541 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3601 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3661 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3721 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3781 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3841 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3901 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3961 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4021 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4081 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4141 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     4201 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     4261 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     4321 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     4381 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     4441 attgtgagcg gataacaatt tcacacgagc tatttaggtg acactataga agtgaagctt
//
