LOCUS pGG-D-attB2_GSrhino-E 3309 bp DNA circular UNA 22-MAR-2023 DEFINITION . ACCESSION urn.local...28-cl8gfo9 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers GG 1..11 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="D" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; D" /label="D" attB2 12..35 /modified_by="mamee" /label="attB2" CDS 65..634 /cds_type="ORF" /label="GSRhino" GG complement(635..645) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="E" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; E" /label="E" primer complement(665..685) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)" /label="GG_SEQ_rev" promoter 1165..1269 /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)" /label="amp" misc_feature 1270..2130 /X-Transferred_From="AmpR" /X-Transferred_Similarity="100.00%" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR" /label="AmpR" /note="Geneious type: Bacterial Selection" rep_origin 2301..2889 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 3253..3274 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)" /label="GG_SEQ_fw" ORIGIN 1 ggtctcatca gacccagctt tcttgtacaa agtggagcag aagcttatct ccgaggagga 61 tctcatggac gaaaagacca ccgggtggcg gggcggccac gttgtggagg gactcgctgg 121 cgagctggag cagctcaggg cccgcttgga gcaccatccc caggggcaac gcgagcctat 181 cgatcttgaa gttctttttc aaggacctct tgaagttctt tttcaaggac ctatcgatga 241 gaacctgtac tttcagggag gtagtacccc ggccgtgacg acctacaagc tggtgatcaa 301 cggcaagacc ctgaagggcg agactacaac caaggccgtg gacgctgaaa ccgcagagaa 361 ggccttcaag cagtacgcca acgacaatgg cgtggacgga gtgtggacct acgacgatgc 421 caccaagacc ttcaccgtga ccgaggttaa cacccccgca gtcaccacat acaaactggt 481 gatcaacgga aaaacgctga agggcgaaac tacgaccaag gctgtggacg cggagacggc 541 cgaaaaggcg ttcaaacagt atgccaacga taacggtgta gacggcgtct ggacatatga 601 cgacgcgaca aagactttca ctgtcactga atagctgctg agaccgaatt ctcgccctat 661 agtgagtcgt attacctggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 721 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 781 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg 841 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt 901 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac 961 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc 1021 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc 1081 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca 1141 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 1201 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 1261 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 1321 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 1381 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 1441 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 1501 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 1561 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 1621 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 1681 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 1741 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 1801 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 1861 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 1921 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 1981 cgtggctctc gaggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 2041 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 2101 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 2161 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 2221 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 2281 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 2341 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 2401 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 2461 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 2521 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 2581 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 2641 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 2701 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 2761 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 2821 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 2881 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 2941 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 3001 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 3061 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 3121 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 3181 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 3241 ttgtgtggaa ttgtgagcgg ataacaattt cacacgagct atttaggtga cactatagaa 3301 gtgaagctt //