LOCUS pGG-D-attB2_GSrhino-E 3309 bp DNA circular UNA 22-MAR-2023
DEFINITION .
ACCESSION urn.local...28-cl8gfo9
KEYWORDS .
SOURCE
ORGANISM .
FEATURES Location/Qualifiers
GG 1..11
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="D"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; D"
/label="D"
attB2 12..35
/modified_by="mamee"
/label="attB2"
CDS 65..634
/cds_type="ORF"
/label="GSRhino"
GG complement(635..645)
/created_by="thjac"
/modified_by="thjac"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="E"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; E"
/label="E"
primer complement(665..685)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
/label="GG_SEQ_rev"
promoter 1165..1269
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)"
/label="amp"
misc_feature 1270..2130
/X-Transferred_From="AmpR"
/X-Transferred_Similarity="100.00%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR"
/label="AmpR"
/note="Geneious type: Bacterial Selection"
rep_origin 2301..2889
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 3253..3274
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
/label="GG_SEQ_fw"
ORIGIN
1 ggtctcatca gacccagctt tcttgtacaa agtggagcag aagcttatct ccgaggagga
61 tctcatggac gaaaagacca ccgggtggcg gggcggccac gttgtggagg gactcgctgg
121 cgagctggag cagctcaggg cccgcttgga gcaccatccc caggggcaac gcgagcctat
181 cgatcttgaa gttctttttc aaggacctct tgaagttctt tttcaaggac ctatcgatga
241 gaacctgtac tttcagggag gtagtacccc ggccgtgacg acctacaagc tggtgatcaa
301 cggcaagacc ctgaagggcg agactacaac caaggccgtg gacgctgaaa ccgcagagaa
361 ggccttcaag cagtacgcca acgacaatgg cgtggacgga gtgtggacct acgacgatgc
421 caccaagacc ttcaccgtga ccgaggttaa cacccccgca gtcaccacat acaaactggt
481 gatcaacgga aaaacgctga agggcgaaac tacgaccaag gctgtggacg cggagacggc
541 cgaaaaggcg ttcaaacagt atgccaacga taacggtgta gacggcgtct ggacatatga
601 cgacgcgaca aagactttca ctgtcactga atagctgctg agaccgaatt ctcgccctat
661 agtgagtcgt attacctggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
721 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
781 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
841 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
901 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
961 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
1021 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
1081 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
1141 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
1201 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
1261 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
1321 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
1381 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
1441 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
1501 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
1561 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
1621 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
1681 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
1741 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
1801 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
1861 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
1921 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
1981 cgtggctctc gaggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
2041 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
2101 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
2161 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
2221 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
2281 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
2341 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
2401 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
2461 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
2521 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
2581 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
2641 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
2701 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
2761 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
2821 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
2881 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
2941 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
3001 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
3061 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
3121 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
3181 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
3241 ttgtgtggaa ttgtgagcgg ataacaattt cacacgagct atttaggtga cactatagaa
3301 gtgaagctt
//