LOCUS pGG-B-N_GSrhino_attB1-C 3286 bp DNA circular UNA 22-MAR-2023
DEFINITION .
ACCESSION urn.local...1o-cl8gfnv
KEYWORDS .
SOURCE
ORGANISM .
FEATURES Location/Qualifiers
GG 1..11
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="B"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; B"
/label="B"
CDS 12..566
/cds_type="ORF"
/label="N_GSRhino"
Linker 567..581
/region_type="Connecting peptide"
/modified_by="mamee"
/label="G4S linker"
attB1 592..611
/modified_by="mamee"
/label="attB1"
GG complement(612..622)
/created_by="thjac"
/modified_by="thjac"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="C"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; C"
/label="C"
primer complement(642..662)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
/label="GG_SEQ_rev"
promoter 1142..1246
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)"
/label="amp"
misc_feature 1247..2107
/X-Transferred_From="AmpR"
/X-Transferred_Similarity="100.00%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR"
/label="AmpR"
/note="Geneious type: Bacterial Selection"
rep_origin 2278..2866
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 3230..3251
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
/label="GG_SEQ_fw"
ORIGIN
1 ggtctcaaac aatggaattc atgggcaccc ccgcagtcac cacctacaag ctggtcatta
61 acggcaagac tctgaagggc gaaaccacca ccaaggccgt ggacgcagaa accgcggaaa
121 aggcgttcaa gcagtacgcg aacgacaacg gggtggacgg agtctggacc tacgatgacg
181 ctacaaaaac gttcaccgtg accgaggtta acacccccgc tgtgactacg tacaagctgg
241 tgatcaatgg gaagaccctg aagggcgaaa ccaccacgaa agctgtagac gccgagacag
301 ccgagaaggc cttcaagcag tacgccaatg acaacggcgt ggatggcgtg tggacctatg
361 acgacgccac gaagacattc actgtgactg agatcgatct tgaagttctt tttcaaggac
421 ctcttgaagt tctttttcaa ggacctggca gtatggacga gaagaccacc ggctggagag
481 gcggccacgt ggtcgaggga ctggccggtg agctggagca gctacgggcc cgccttgagc
541 accaccccca ggggcagcgc gagccgggtg gaggcggttc actcgagatc acaagtttgt
601 acaaaaaagc aggcttgaga ccgaattctc gccctatagt gagtcgtatt acctggccgt
661 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
721 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
781 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
841 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
901 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
961 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
1021 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
1081 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
1141 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
1201 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
1261 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
1321 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
1381 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
1441 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
1501 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
1561 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
1621 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
1681 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
1741 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
1801 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
1861 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
1921 tggctggttt attgctgata aatctggagc cggtgagcgt ggctctcgag gtatcattgc
1981 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
2041 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
2101 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
2161 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
2221 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
2281 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
2341 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
2401 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
2461 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
2521 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
2581 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
2641 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
2701 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
2761 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
2821 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
2881 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
2941 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
3001 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
3061 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
3121 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
3181 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
3241 acaatttcac acgagctatt taggtgacac tatagaagtg aagctt
//