LOCUS pGG-B-N_GSrhino_attB1-C 3286 bp DNA circular UNA 22-MAR-2023 DEFINITION . ACCESSION urn.local...1o-cl8gfnv KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers GG 1..11 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="B" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; B" /label="B" CDS 12..566 /cds_type="ORF" /label="N_GSRhino" Linker 567..581 /region_type="Connecting peptide" /modified_by="mamee" /label="G4S linker" attB1 592..611 /modified_by="mamee" /label="attB1" GG complement(612..622) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="C" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; C" /label="C" primer complement(642..662) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)" /label="GG_SEQ_rev" promoter 1142..1246 /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)" /label="amp" misc_feature 1247..2107 /X-Transferred_From="AmpR" /X-Transferred_Similarity="100.00%" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR" /label="AmpR" /note="Geneious type: Bacterial Selection" rep_origin 2278..2866 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 3230..3251 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)" /label="GG_SEQ_fw" ORIGIN 1 ggtctcaaac aatggaattc atgggcaccc ccgcagtcac cacctacaag ctggtcatta 61 acggcaagac tctgaagggc gaaaccacca ccaaggccgt ggacgcagaa accgcggaaa 121 aggcgttcaa gcagtacgcg aacgacaacg gggtggacgg agtctggacc tacgatgacg 181 ctacaaaaac gttcaccgtg accgaggtta acacccccgc tgtgactacg tacaagctgg 241 tgatcaatgg gaagaccctg aagggcgaaa ccaccacgaa agctgtagac gccgagacag 301 ccgagaaggc cttcaagcag tacgccaatg acaacggcgt ggatggcgtg tggacctatg 361 acgacgccac gaagacattc actgtgactg agatcgatct tgaagttctt tttcaaggac 421 ctcttgaagt tctttttcaa ggacctggca gtatggacga gaagaccacc ggctggagag 481 gcggccacgt ggtcgaggga ctggccggtg agctggagca gctacgggcc cgccttgagc 541 accaccccca ggggcagcgc gagccgggtg gaggcggttc actcgagatc acaagtttgt 601 acaaaaaagc aggcttgaga ccgaattctc gccctatagt gagtcgtatt acctggccgt 661 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 721 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 781 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct 841 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata 901 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct 961 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt 1021 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata 1081 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt 1141 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 1201 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 1261 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 1321 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 1381 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 1441 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 1501 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 1561 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 1621 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 1681 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 1741 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 1801 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 1861 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 1921 tggctggttt attgctgata aatctggagc cggtgagcgt ggctctcgag gtatcattgc 1981 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 2041 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 2101 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 2161 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 2221 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 2281 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 2341 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 2401 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 2461 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 2521 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 2581 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 2641 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 2701 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 2761 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 2821 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 2881 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 2941 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 3001 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg 3061 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 3121 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc 3181 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 3241 acaatttcac acgagctatt taggtgacac tatagaagtg aagctt //