LOCUS       pGGW-C-m43GW-D          4991 bp    DNA     circular UNA 21-MAR-2023
DEFINITION  .
ACCESSION   urn.local...1d9-cixtyol
VERSION     urn.local...1d9-cixtyol
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:88-dgycryy"">C</a>"
                     /label="C"
     attR4           68..191
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR4"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:l1-eztyg66"">attR4</a>"
                     /Alternative_Match="99.19%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:o4-f0pbp83"">attR4</a>"
     CDS             complement(232..537)
                     /standard_name="ccdB"
     UTR             complement(538..856)
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.37%"
                     /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    complement(883..1542)
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="14 CmR"
                     /Transferred_Similarity="99.85%"
                     /Primary_Match="99.85%; Adjusted-Interval-0:0/0; CmR (<a
                     href=""urn:local:.:1h-fz09kes"">14 CmR</a>)"
                     /Alternative_Match="99.85%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     promoter        complement(1596..1626)
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="14 CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:1h-fz09kes"">14 CmR</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacUV5 (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     attR3           complement(1650..1774)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:kw-eztyb8e"">attR3</a>"
     GG              complement(1818..1828)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:81-dgycryx"">D</a>"
                     /label="D"
     misc_feature    complement(2604..3137)
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="GmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8k-dgycryy"">GmR</a>"
                     /label="GmR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      4011..4599
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
ORIGIN      
        1 ggtctcaggc tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
       61 aagctatcaa ctttgtatag aaaagttgaa cgagaaacgt aaaatgatat aaatatcaat
      121 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
      181 cagtcactat ggtcgacctg cagactggct gtgtataagg gagcctgaca tttatattcc
      241 ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac
      301 ttcttccccg ataacggaga ccggcacact ggccatatcg gtggtcatca tgcgccagct
      361 ttcatccccg atatgcacca ccgggtaaag ttcacgggag actttatctg acagcagacg
      421 tgcactggcc agggggatca ccatccgtcg cccgggcgtg tcaataatat cactctgtac
      481 atccacaaac agacgataac ggctctctct tttataggtg taaaccttaa actgcatttc
      541 accagtccct gttctcgtca gcaaaagagc cgttcatttc aataaaccgg gcgacctcag
      601 ccatcccttc ctgattttcc gctttccagc gttcggcacg cagacgacgg gcttcattct
      661 gcatggttgt gcttaccaga ccggagatat tgacatcata tatgccttga gcaactgata
      721 gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata gcacacctct ttttgacata
      781 cttcgggtat acatatcagt atatattctt ataccgcaaa aatcagcgcg caaatacgca
      841 tactgttatc tggcttttag taagccggat cgatccacgc gtttacgccc cgccctgcca
      901 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacagac
      961 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     1021 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     1081 ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg
     1141 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     1201 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     1261 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     1321 acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     1381 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     1441 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
     1501 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
     1561 tgaaaatctc gccggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag
     1621 tgtaaagcct ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag
     1681 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
     1741 tacgtttctc gttcaacttt attatacata gttgataatt cactggccgt cgttttacaa
     1801 cgtcgtgact gggaaaatca gtgagacccg agctcctctg gcccgtgtct caaaatctct
     1861 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
     1921 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat
     1981 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     2041 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga
     2101 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
     2161 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     2221 ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg
     2281 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
     2341 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
     2401 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
     2461 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca
     2521 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ggccgggaag
     2581 ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc
     2641 acttcttccc gtatgcccaa ctttgtatag agagccactg cgggatcgtc accgtaatct
     2701 gcttgcacgt agatcacata agcaccaagc gcgttggcct catgcttgag gagattgatg
     2761 agcgcggtgg caatgccctg cctccggtgc tcgccggaga ctgcgagatc atagatatag
     2821 atctcactac gcggctgctc aaacctgggc agaacgtaag ccgcgagagc gccaacaacc
     2881 gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa gttcccgagg
     2941 taatcggagt ccggctgatg ttgggagtag gtggctacgt ctccgaactc acgaccgaaa
     3001 agatcaagag cagcccgcat ggatttgact tggtcagggc cgagcctaca tgtgcgaatg
     3061 atgcccatac ttgagccacc taactttgtt ttagggcgac tgccctgctg cgtaacatcg
     3121 ttgctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
     3181 cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat
     3241 gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg
     3301 tgagcgcata cgctacttgc attacagttt acgaaccgaa caggcttatg tcaactgggt
     3361 tcgtgccttc atccgtttcc acggtgtgcg tcacccggca accttgggca gcagcgaagt
     3421 cgaggcattt ctgtcctggc tggcgaacga gcgcaaggtt tcgctctcca cgcatcgtca
     3481 ggcattggcg gccttgctgt tcttctacgg caaggtgctg tgcacggatc tgccctggct
     3541 tcaggagatc ggaagacctc ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga
     3601 agtggttcgc atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc aggactctag
     3661 ctatagttct agtggttggc tactaatagg ttgtattgat gttggacgag tcggaatcgc
     3721 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     3781 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     3841 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca
     3901 gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag
     3961 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4021 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4081 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     4141 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     4201 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4261 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4321 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4381 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4441 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4501 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4561 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4621 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     4681 gattctgtgg ataaccgtat taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     4741 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     4801 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     4861 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     4921 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     4981 atctcgggcc c
//
