LOCUS       pCDB-Cas9-GFP           9735 bp    DNA     circular UNA 26-AUG-2022
DEFINITION  .
ACCESSION   urn.local...o-cixsr3o
VERSION     urn.local...o-cixsr3o
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     attL1           1..100
                     /Color="#00cccc"
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL1"
                     /Transferred_Similarity="99.00%"
                     /Primary_Match="99.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:li-eztyqs1"">attL1</a>"
                     /label="attL1"
     promoter        107..970
                     /regulatory_class="promoter"
                     /gene="CaMV 35S"
                     /note="internal BsaI recognition site removed"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCaMV 35S"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6v-dgycryx"">pCaMV 35S</a>"
                     /modified_by="mamee"
                     /label="p35S"
     Linker          975..998
                     /gene="B-dummy"
                     /note="default module for GreenGate"
                     /modified_by="mamee"
                     /label="B-dummy 5'UTR"
     CDS             1005..5108
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AtCas9"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:95-dgycryy"">AtCas9</a>"
                     /label="AtCAS9"
     NLS             5121..5141
                     /created_by="warddecaestecker"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS SV40"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (<a
                     href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /label="SV40"
     CDS             5148..5966
                     /codon_start=1
                     /transl_table=11
                     /product="linker-GFP"
                     /protein_id="AHE38523.1"
                     /translation="AASAIASGSGSGSGSGSGSGSGSGSGSGSAAAAMVSKGEELFTG
                     VVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFTYGV
                     QCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELK
                     GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQ
                     NTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK"
                     /modified_by="mamee"
                     /label="linker-GFP"
     terminator      5971..6617
                     /regulatory_class="terminator"
                     /note="RBCS terminator (from Pisum sativum)"
                     /modified_by="mamee"
                     /label="tRbcS"
     promoter        6624..7011
                     /vntifkey=29
                     /modified_by="thjac"
                     /label="AtU6-26"
     Scaffold        7036..7118
                     /%_Identity=100
                     /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
                     TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
                     /annotation_group="Scaffold: 451 -> 533"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpCas9 Scaffold"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Scaffold
                     (<a href=""urn:local:.:7o-dgycryx"">SpCas9 Scaffold</a>)"
                     /label="Scaffold"
     attL2           complement(7124..7223)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ll-eztyzh8"">attL2</a>"
                     /label="attL2"
     primer          complement(7261..7281)
                     /label="GWM13R"
     promoter        7342..7392
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    7393..8202
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      8349..8937
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     misc_feature    9486..9653
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer          9704..9724
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct taacctccga tccattcggt
      121 ccccagatta gccttttcaa tttcagaaag aatgctaacc cacagatggt tagagaggct
      181 tacgcagcac gtctcatcaa gacgatctac ccgagcaata atctccagga aatcaaatac
      241 cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaactgcat caagaacaca
      301 gagaaagata tatttctcaa gatcagaagt actattccag tatggacgat tcaaggcttg
      361 cttcacaaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt tcccactgaa
      421 tcaaaggcca tggagtcaaa gattcaaata gaggacctaa cagaactcgc cgtaaagact
      481 ggcgaacagt tcatacagag tctcttacga ctcaatgaca agaagaaaat cttcgtcaac
      541 atggtggagc acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac
      601 caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat
      661 tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa
      721 tgccatcatt gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc
      781 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
      841 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
      901 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gagaacacgg
      961 gggacgagct aacagtattc agtcgactgg taccaacagg ctcgatggat aagaagtact
     1021 ctatcggact cgatatcgga actaactctg tgggatgggc tgtgatcacc gatgagtaca
     1081 aggtgccatc taagaagttc aaggttctcg gaaacaccga taggcactct atcaagaaaa
     1141 accttatcgg tgctctcctc ttcgattctg gtgaaactgc tgaggctacc agactcaaga
     1201 gaaccgctag aagaaggtac accagaagaa agaacaggat ctgctacctc caagagatct
     1261 tctctaacga gatggctaaa gtggatgatt cattcttcca caggctcgaa gagtcattcc
     1321 tcgtggaaga agataagaag cacgagaggc accctatctt cggaaacatc gttgatgagg
     1381 tggcatacca cgagaagtac cctactatct accacctcag aaagaagctc gttgattcta
     1441 ctgataaggc tgatctcagg ctcatctacc tcgctctcgc tcacatgatc aagttcagag
     1501 gacacttcct catcgagggt gatctcaacc ctgataactc tgatgtggat aagttgttca
     1561 tccagctcgt gcagacctac aaccagcttt tcgaagagaa ccctatcaac gcttcaggtg
     1621 tggatgctaa ggctatcctc tctgctaggc tctctaagtc aagaaggctt gagaacctca
     1681 ttgctcagct ccctggtgag aagaagaacg gacttttcgg aaacttgatc gctctctctc
     1741 tcggactcac ccctaacttc aagtctaact tcgatctcgc tgaggatgca aagctccagc
     1801 tctcaaagga tacctacgat gatgatctcg ataacctcct cgctcagatc ggagatcagt
     1861 acgctgattt gttcctcgct gctaagaacc tctctgatgc tatcctcctc agtgatatcc
     1921 tcagagtgaa caccgagatc accaaggctc cactctcagc ttctatgatc aagagatacg
     1981 atgagcacca ccaggatctc acacttctca aggctcttgt tagacagcag ctcccagaga
     2041 agtacaaaga gattttcttc gatcagtcta agaacggata cgctggttac atcgatggtg
     2101 gtgcatctca agaagagttc tacaagttca tcaagcctat cctcgagaag atggatggaa
     2161 ccgaggaact cctcgtgaag ctcaatagag aggatcttct cagaaagcag aggaccttcg
     2221 ataacggatc tatccctcat cagatccacc tcggagagtt gcacgctatc cttagaaggc
     2281 aagaggattt ctacccattc ctcaaggata acagggaaaa gattgagaag attctcacct
     2341 tcagaatccc ttactacgtg ggacctctcg ctagaggaaa ctcaagattc gcttggatga
     2401 ccagaaagtc tgaggaaacc atcacccctt ggaacttcga agaggtggtg gataagggtg
     2461 ctagtgctca gtctttcatc gagaggatga ccaacttcga taagaacctt ccaaacgaga
     2521 aggtgctccc taagcactct ttgctctacg agtacttcac cgtgtacaac gagttgacca
     2581 aggttaagta cgtgaccgag ggaatgagga agcctgcttt tttgtcaggt gagcaaaaga
     2641 aggctatcgt tgatctcttg ttcaagacca acagaaaggt gaccgtgaag cagctcaaag
     2701 aggattactt caagaaaatc gagtgcttcg attcagttga gatttctggt gttgaggata
     2761 ggttcaacgc atctctcgga acctaccacg atctcctcaa gatcattaag gataaggatt
     2821 tcttggataa cgaggaaaac gaggatatct tggaggatat cgttcttacc ctcaccctct
     2881 ttgaagatag agagatgatt gaagaaaggc tcaagaccta cgctcatctc ttcgatgata
     2941 aggtgatgaa gcagttgaag agaagaagat acactggttg gggaaggctc tcaagaaagc
     3001 tcattaacgg aatcagggat aagcagtctg gaaagacaat ccttgatttc ctcaagtctg
     3061 atggattcgc taacagaaac ttcatgcagc tcatccacga tgattctctc acctttaaag
     3121 aggatatcca gaaggctcag gtttcaggac agggtgatag tctccatgag catatcgcta
     3181 acctcgctgg atctcctgca atcaagaagg gaatcctcca gactgtgaag gttgtggatg
     3241 agttggtgaa ggtgatggga aggcataagc ctgagaacat cgtgatcgaa atggctagag
     3301 agaaccagac cactcagaag ggacagaaga actctaggga aaggatgaag aggatcgagg
     3361 aaggtatcaa agagcttgga tctcagatcc tcaaagagca ccctgttgag aacactcagc
     3421 tccagaatga gaagctctac ctctactacc tccagaacgg aagggatatg tatgtggatc
     3481 aagagttgga tatcaacagg ctctctgatt acgatgttga tcatatcgtg ccacagtcat
     3541 tcttgaagga tgattctatc gataacaagg tgctcaccag gtctgataag aacaggggta
     3601 agagtgataa cgtgccaagt gaagaggttg tgaagaaaat gaagaactat tggaggcagc
     3661 tcctcaacgc taagctcatc actcagagaa agttcgataa cttgactaag gctgagaggg
     3721 gaggactctc tgaattggat aaggcaggat tcatcaagag gcagcttgtg gaaaccaggc
     3781 agatcactaa gcacgttgca cagatcctcg attctaggat gaacaccaag tacgatgaga
     3841 acgataagtt gatcagggaa gtgaaggtta tcaccctcaa gtcaaagctc gtgtctgatt
     3901 tcagaaagga tttccaattc tacaaggtga gggaaatcaa caactaccac cacgctcacg
     3961 atgcttacct taacgctgtt gttggaaccg ctctcatcaa gaagtatcct aagctcgagt
     4021 cagagttcgt gtacggtgat tacaaggtgt acgatgtgag gaagatgatc gctaagtctg
     4081 agcaagagat cggaaaggct accgctaagt atttcttcta ctctaacatc atgaatttct
     4141 tcaagaccga gattaccctc gctaacggtg agatcagaaa gaggccactc atcgagacaa
     4201 acggtgaaac aggtgagatc gtgtgggata agggaaggga tttcgctacc gttagaaagg
     4261 tgctctctat gccacaggtg aacatcgtta agaaaaccga ggtgcagacc ggtggattct
     4321 ctaaagagtc tatcctccct aagaggaact ctgataagct cattgctagg aagaaggatt
     4381 gggaccctaa gaaatacggt ggtttcgatt ctcctaccgt ggcttactct gttctcgttg
     4441 tggctaaggt tgagaaggga aagagtaaga agctcaagtc tgttaaggaa cttctcggaa
     4501 tcactatcat ggaaaggtca tctttcgaga agaacccaat cgatttcctc gaggctaagg
     4561 gatacaaaga ggttaagaag gatctcatca tcaagctccc aaagtactca ctcttcgaac
     4621 tcgagaacgg tagaaagagg atgctcgctt ctgctggtga gcttcaaaag ggaaacgagc
     4681 ttgctctccc atctaagtac gttaactttc tttacctcgc ttctcactac gagaagttga
     4741 agggatctcc agaagataac gagcagaagc aacttttcgt tgagcagcac aagcactact
     4801 tggatgagat catcgagcag atctctgagt tctctaaaag ggtgatcctc gctgatgcaa
     4861 acctcgataa ggtgttgtct gcttacaaca agcacagaga taagcctatc agggaacagg
     4921 cagagaacat catccatctc ttcaccctta ccaacctcgg tgctcctgct gctttcaagt
     4981 acttcgatac aaccatcgat aggaagagat acacctctac caaagaagtg ctcgatgcta
     5041 ccctcatcca tcagtctatc actggactct acgagactag gatcgatctc tcacagctcg
     5101 gtggtgattc aagggctgat cctaagaaga agaggaaggt ttcaggtgcc gctagtgcga
     5161 tcgcatcagg gagtggttcc ggaagcggct ctggatcggg ctcagggagt ggttccggca
     5221 gcggctctgg atcggcggcc gctgcaatgg tgagcaaggg cgaggagctg ttcaccgggg
     5281 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     5341 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     5401 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cacctacggc gtgcagtgct
     5461 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     5521 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     5581 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     5641 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     5701 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     5761 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     5821 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     5881 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     5941 acggcatgga cgagctgtac aagtagctgc aaagctttcg ttcgtatcat cggtttcgac
     6001 aacgttcgtc aagttcaatg catcagtttc attgcgcaca caccagaatc ctactgagtt
     6061 tgagtattat ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta
     6121 ctgtgttttt tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt
     6181 taatgaatga tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta
     6241 tttgttgtgt gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa
     6301 atgtgtcaaa tcgtggcctc taatgaccga agttaatatg aggagtaaaa cacttgtagt
     6361 tgtaccatta tgcttattca ctaggcaaca aatatatttt cagacctaga aaagctgcaa
     6421 atgttactga atacaagtat gtcctcttgt gttttagaca tttatgaact ttcctttatg
     6481 taattttcca gaatccttgt cagattctaa tcattgcttt ataattatag ttatactcat
     6541 ggatttgtag ttgagtatga aaatattttt taatgcattt tatgacttgc caattgattg
     6601 acaacatgca tcaatcgact actctttttt tcttcttctt cgttcataca gttttttttt
     6661 gtttatcagc ttacattttc ttgaaccgta gctttcgttt tcttcttttt aactttccat
     6721 tcggagtttt tgtatcttgt ttcatagttt gtcccaggat tagaatgatt aggcatcgaa
     6781 ccttcaagaa tttgattgaa taaaacatct tcattcttaa gatatgaaga taatcttcaa
     6841 aaggcccctg ggaatctgaa agaagagaag caggcccatt tatatgggaa agaacaatag
     6901 tatttcttat ataggcccat ttaagttgaa aacaatcttc aaaagtccca catcgcttag
     6961 ataagaaaac gaagctgagt ttatatacag ctagagtcga agtagtgatt gggatgcagg
     7021 tggacacctg cagctgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
     7081 tcaacttgaa aaagtggcac cgagtcggtg ctttttttgt attacccagc tttcttgtac
     7141 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
     7201 agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac
     7261 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     7321 gcacaagata aaataatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     7381 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac
     7441 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     7501 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     7561 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     7621 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     7681 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     7741 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     7801 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     7861 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     7921 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     7981 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     8041 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     8101 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     8161 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     8221 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
     8281 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8341 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8401 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8461 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     8521 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8581 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8641 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8701 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8761 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8821 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     8881 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8941 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     9001 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     9061 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     9121 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     9181 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct
     9241 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag
     9301 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca
     9361 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
     9421 aacagataaa acgaaaggcc cagacttccg actgagcctt tcgttttatt tgatgcctgg
     9481 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     9541 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     9601 gaaaggccca gacttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     9661 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
     9721 cttaagctcg ggccc
//
