LOCUS       pK7m34GW-p35s-GFP-linkerTurboID       11681 bp    DNA     circular UNA 20-MAR-2023
DEFINITION  .
ACCESSION   urn.local...l4-citr5zy
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     http://www.informaxinc.com/.
FEATURES             Location/Qualifiers
     Promoter        8..289
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="96.81%"
                     /Primary_Match="96.81%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    290..1081
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1325..1913
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     regulatory      complement(1949..2209)
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /label="pBR322 bom"
     rep_origin      2583..2777
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     regulatory      complement(2843..3915)
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="99.91%"
                     /Primary_Match="99.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     regulatory      complement(4344..4973)
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     T-DNA_border    6159..6358
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:3c-djznfva"">RB</a>"
                     /label="RB"
     primer          6386..6402
                     /invitrogen=2010000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 reverse primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     reverse primer (pDONRP4-P3 - M13 reverse primer)"
                     /label="M13 reverse primer"
     promoter        6445..7567
                     /vntifkey=29
                     /note="35S Cauliflower mosaic virus promotor"
                     /label="P35S"
     CDS             7595..8311
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="EGFP"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:hy-e4twm0o"">EGFP</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Egfp
                     (<a href=""urn:local:.:fg-eaul7he"">8 Egfp</a>)"
                     /Alternative_Match="99.16%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8y-dgycryy"">GFP</a>"
                     /Alternative_Match="99.02%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:hh-e4tw8wz"">ECFP</a>"
                     /Alternative_Match="99.02%; Adjusted-Interval-0:0/0; Ecfp
                     (<a href=""urn:local:.:ff-eaul7he"">9 ECFP</a>)"
                     /Alternative_Match="98.61%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ie-e4twtil"">EYFP</a>"
                     /Alternative_Match="98.61%; Adjusted-Interval-0:0/0; Eyfp
                     (<a href=""urn:local:.:fh-eaul7he"">10 EYFP</a>)"
                     /label="EGFP"
     Linker          8339..8533
                     /modified_by="mamee"
                     /label="(GGGGS)13 linker"
     CDS             8537..9499
                     /label="TurboID"
     tag             9503..9583
                     /modified_by="mamee"
                     /label="3xHA"
     primer          complement(9617..9632)
                     /invitrogen=1980000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-20) forward primer (pDONRP4-P3 - M13 (-20) forward
                     primer)"
                     /label="M13 (-20) forward primer"
     primer          complement(9636..9652)
                     /invitrogen=2000000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-40) forward primer (pDONRP4-P3 - M13 (-40) forward
                     primer)"
                     /label="M13 (-40) forward primer"
     terminator      9676..9915
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="35ST"
                     /Transferred_Similarity="95.42%"
                     /Primary_Match="95.42%; Adjusted-Interval-0:0/0; t35S (<a
                     href=""urn:local:.:6d-dgycryw"">35ST</a>)"
                     /label="t35S"
     Plant_Selection 9940..11328
                     /created_by="mamee"
                     /label="Kana"
     promoter        9940..10227
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pNOS"
                     /Transferred_Similarity="94.79%"
                     /Primary_Match="94.79%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:77-dgycryx"">pNOS</a>"
                     /label="pNOS"
     CDS             10240..11034
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="NptII"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:94-dgycryy"">NptII</a>"
                     /label="NptII"
     terminator      11061..11328
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="NOST"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (<a
                     href=""urn:local:.:6c-dgycryw"">NOST</a>)"
                     /label="tNOS"
     T-DNA_border    11349..11681
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
ORIGIN      
        1 attcgggcac gaacccagtg gacataagcc tcgttcggtt cgtaagctgt aatgcaagta
       61 gcgtaactgc cgtcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
      121 gcagtggcgg ttttcatggc ttcttgttat gacatgtttt tttggggtac agtctatgcc
      181 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
      241 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc
      301 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
      361 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
      421 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
      481 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
      541 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
      601 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
      661 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
      721 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
      781 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
      841 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
      901 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
      961 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     1021 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     1081 atgtctagct agaaattcgt tcaagccgac gccgcttcgc cggcgttaac tcaagcgatt
     1141 agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta
     1201 tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa
     1261 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1321 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1381 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1441 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     1501 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1561 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1621 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1681 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1741 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1801 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1861 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1921 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1981 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2041 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2101 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     2161 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     2221 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     2281 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     2341 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc
     2401 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
     2461 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
     2521 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
     2581 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
     2641 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
     2701 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
     2761 tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt
     2821 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
     2881 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
     2941 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
     3001 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
     3061 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
     3121 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
     3181 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
     3241 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
     3301 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
     3361 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
     3421 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
     3481 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
     3541 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
     3601 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
     3661 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
     3721 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
     3781 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
     3841 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
     3901 gtttgcgacc gtcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
     3961 cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
     4021 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
     4081 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
     4141 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
     4201 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
     4261 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
     4321 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
     4381 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
     4441 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
     4501 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
     4561 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
     4621 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
     4681 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
     4741 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
     4801 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
     4861 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
     4921 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
     4981 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
     5041 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
     5101 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
     5161 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
     5221 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
     5281 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
     5341 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
     5401 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
     5461 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
     5521 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
     5581 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
     5641 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
     5701 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
     5761 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
     5821 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
     5881 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
     5941 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
     6001 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
     6061 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
     6121 gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg
     6181 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
     6241 ccgttattct aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac
     6301 actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctaagcttg
     6361 ggcccgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcta
     6421 tcaactttgt atagaaaagt tgtcatttag gtgacactat agaatactca agctatgcat
     6481 ccaacgcgtt gggagctctc ccatatggtc gactagagcc aagctgatct cctttgcccc
     6541 ggagatcacc atggacgact ttctctatct ctacgatcta ggaagaaagt tcgacggaga
     6601 aggtgacgat accatgttca ccaccgataa tgagaagatt agcctcttca atttcagaaa
     6661 gaatgctgac ccacagatgg ttagagaggc ctacgcggca ggtctcatca agacgatcta
     6721 cccgagtaat aatctccagg agatcaaata ccttcccaag aaggttaaag atgcagtcaa
     6781 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     6841 tactattcca gtatggacga ttcaaggctt gcttcataaa ccaaggcaag taatagagat
     6901 tggagtctct aagaaagtag ttcctactga atcaaaggcc atggagtcaa aaattcagat
     6961 cgaggatcta acagaactcg ccgtgaagac tggcgaacag ttcatacaga gtcttttacg
     7021 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacactc tcgtctactc
     7081 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
     7141 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag
     7201 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat
     7261 cgttcaagat gcccctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     7321 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     7381 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     7441 aggaagttca tttcatttgg agaggactcc ggtattttta caacaattac cacaacaaaa
     7501 caaacaacaa acaacattac aatttactat tctagtcgac ctgcaggcgg ccgcactagt
     7561 gatatcagca agtttgtaca aaaaagcagg ctcaatggtg agcaagggcg aggagctgtt
     7621 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     7681 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     7741 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     7801 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     7861 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     7921 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     7981 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     8041 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     8101 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     8161 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     8221 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     8281 gatcactctc ggcatggacg agctgtacaa ggacccagct ttcttgtaca aagtgggagg
     8341 tggcggaggt tctggcggtg gaggatccgg aggtggcggg tccggtggtg gcggatcagg
     8401 tggaggaggt tcgggaggcg gaggtagtgg aggcggtggg agcggcggag gtgggagcgg
     8461 tggcggaggg agtggaggtg gaggctcggg cggaggtggc tccggcggag gtgggtctgg
     8521 aggcggtggc tcggacaaag acaataccgt gcccctcaaa ctaattgcat tgcttgccaa
     8581 tggtgagttc cattctgggg aacaacttgg ggagactctg ggcatgtccc gagcggccat
     8641 taataagcac atacagactt tgagggactg gggtgtcgat gtgtttacag taccgggtaa
     8701 gggatattct ctgccggagc cgataccttt actcaatgca aagcagattt taggacagct
     8761 ggatggcggg agcgtggctg tcctcccggt ggtcgatagt acaaatcaat atcttttgga
     8821 caggatcggt gagctgaaat ctggagatgc ttgcatagcg gaataccaac aagctggtag
     8881 gggatctcga ggccgaaagt ggttttcacc ctttggggcc aacttatact tatccatgtt
     8941 ctggcgtctg aaacgtggcc cggcagccat agggctaggg cccgtaattg ggatcgttat
     9001 ggcggaagcg ttacgaaagt tgggagccga taaagtcaga gtaaagtggc caaatgatct
     9061 ttatttacaa gatagaaagc tggcgggcat tcttgttgag cttgccggaa taacaggcga
     9121 tgccgcacag atcgttatag gggccgggat taatgtggct atgaggaggg tcgaagaaag
     9181 tgttgtgaat caaggatgga ttactctcca agaggcgggt ataaatcttg atcgaaatac
     9241 tctagctgca accttgatac gagagctaag agcagcacta gaattgtttg aacaagaagg
     9301 gctggcccca tatctccctc gatgggagaa gttggacaat tttataaaca gaccggtgaa
     9361 gttaatcatt ggagacaaag aaattttcgg tataagccgt gggatcgata aacagggtgc
     9421 tcttctcctt gagcaggacg gtgtcatcaa gccttggatg ggaggcgaaa tctctctccg
     9481 aagcgccgag aagaagcttg cctacccata cgatgttcca gattacgctt acccatacga
     9541 tgttccagat tacgcttacc catacgatgt tccagattac gcttgaccaa ctttattata
     9601 catagttgat aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa acgatctaac
     9661 tgactagccg cggccatggc ggccgggagc ggccatgcta gagtccgcaa aaatcaccag
     9721 tctctctcta caaatctatc tctctctatt tttctccaga ataatgtgtg agtagttccc
     9781 agataaggga attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc
     9841 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
     9901 aaaatccagt gacctgcagg tcgaccatat gggagagctc gaattatcat acatgagaat
     9961 taagggagtc acgttatgac ccccgccgat gacgcgggac aagccgtttt acgtttggaa
    10021 ctgacagaac cgcaacgttg aaggagccac tcagccgcgg gtttctggag tttaatgagc
    10081 taagcacata cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg tcactatcag
    10141 ctagcaaata tttcttgtca aaaatgctcc actgacgttc cataaattcc cctcggtatc
    10201 caattagagt ctcatattca ctctcaactc gatcgaggca tgattgaaca agatggattg
    10261 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
    10321 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
    10381 tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc aagacgaggc agcgcggcta
    10441 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
    10501 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
    10561 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
    10621 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
    10681 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
    10741 gccgaactgt tcgccaggct caaggcgcgg atgcccgacg gcgaggatct cgtcgtgacc
    10801 cacggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
    10861 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
    10921 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
    10981 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
    11041 ctctggggtt cggactctag ctagagtcaa gcagatcgtt caaacatttg gcaataaagt
    11101 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
    11161 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
    11221 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
    11281 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgacc ggcatgcaag
    11341 ctgataattc aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag
    11401 ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct
    11461 ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga
    11521 cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt
    11581 cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca
    11641 tgttgattgt aacgatgaca gagcgttgct gcctgtgatc a
//
