LOCUS       pK7m34GW-p35s-GFP-BioID       11483 bp    DNA     circular UNA 20-MAR-2023
DEFINITION  .
ACCESSION   urn.local...l2-citr5zy
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     http://www.informaxinc.com/.
FEATURES             Location/Qualifiers
     Promoter        8..289
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="96.81%"
                     /Primary_Match="96.81%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    290..1081
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1325..1913
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     regulatory      complement(1949..2209)
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /label="pBR322 bom"
     rep_origin      2583..2777
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     regulatory      complement(2843..3915)
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="99.91%"
                     /Primary_Match="99.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     regulatory      complement(4344..4973)
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     T-DNA_border    6159..6358
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:3c-djznfva"">RB</a>"
                     /label="RB"
     primer          6386..6402
                     /invitrogen=2010000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 reverse primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     reverse primer (pDONRP4-P3 - M13 reverse primer)"
                     /label="M13 reverse primer"
     promoter        6445..7567
                     /vntifkey=29
                     /note="35S Cauliflower mosaic virus promotor"
                     /label="P35S"
     CDS             7595..8311
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="EGFP"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:hy-e4twm0o"">EGFP</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Egfp
                     (<a href=""urn:local:.:fg-eaul7he"">8 Egfp</a>)"
                     /Alternative_Match="99.16%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8y-dgycryy"">GFP</a>"
                     /Alternative_Match="99.02%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:hh-e4tw8wz"">ECFP</a>"
                     /Alternative_Match="99.02%; Adjusted-Interval-0:0/0; Ecfp
                     (<a href=""urn:local:.:ff-eaul7he"">9 ECFP</a>)"
                     /Alternative_Match="98.61%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ie-e4twtil"">EYFP</a>"
                     /Alternative_Match="98.61%; Adjusted-Interval-0:0/0; Eyfp
                     (<a href=""urn:local:.:fh-eaul7he"">10 EYFP</a>)"
                     /label="EGFP"
     CDS             8342..9301
                     /label="BioID"
     tag             9305..9385
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="3xHA"
     primer          complement(9419..9434)
                     /invitrogen=1980000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-20) forward primer (pDONRP4-P3 - M13 (-20) forward
                     primer)"
                     /label="M13 (-20) forward primer"
     primer          complement(9438..9454)
                     /invitrogen=2000000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-40) forward primer (pDONRP4-P3 - M13 (-40) forward
                     primer)"
                     /label="M13 (-40) forward primer"
     terminator      9478..9717
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="35ST"
                     /Transferred_Similarity="95.42%"
                     /Primary_Match="95.42%; Adjusted-Interval-0:0/0; t35S (<a
                     href=""urn:local:.:6d-dgycryw"">35ST</a>)"
                     /label="t35S"
     Plant_Selection 9742..11130
                     /created_by="mamee"
                     /label="Kana"
     promoter        9742..10029
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pNOS"
                     /Transferred_Similarity="94.79%"
                     /Primary_Match="94.79%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:77-dgycryx"">pNOS</a>"
                     /label="pNOS"
     CDS             10042..10836
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="NptII"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:94-dgycryy"">NptII</a>"
                     /label="NptII"
     terminator      10863..11130
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="NOST"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (<a
                     href=""urn:local:.:6c-dgycryw"">NOST</a>)"
                     /label="tNOS"
     T-DNA_border    11151..11483
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
ORIGIN      
        1 attcgggcac gaacccagtg gacataagcc tcgttcggtt cgtaagctgt aatgcaagta
       61 gcgtaactgc cgtcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
      121 gcagtggcgg ttttcatggc ttcttgttat gacatgtttt tttggggtac agtctatgcc
      181 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
      241 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc
      301 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
      361 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
      421 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
      481 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
      541 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
      601 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
      661 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
      721 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
      781 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
      841 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
      901 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
      961 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     1021 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     1081 atgtctagct agaaattcgt tcaagccgac gccgcttcgc cggcgttaac tcaagcgatt
     1141 agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta
     1201 tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa
     1261 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1321 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1381 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1441 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     1501 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1561 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1621 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1681 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1741 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1801 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1861 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1921 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1981 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2041 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2101 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     2161 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     2221 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     2281 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     2341 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc
     2401 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
     2461 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
     2521 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
     2581 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
     2641 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
     2701 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
     2761 tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt
     2821 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
     2881 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
     2941 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
     3001 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
     3061 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
     3121 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
     3181 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
     3241 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
     3301 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
     3361 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
     3421 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
     3481 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
     3541 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
     3601 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
     3661 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
     3721 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
     3781 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
     3841 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
     3901 gtttgcgacc gtcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
     3961 cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
     4021 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
     4081 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
     4141 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
     4201 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
     4261 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
     4321 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
     4381 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
     4441 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
     4501 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
     4561 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
     4621 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
     4681 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
     4741 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
     4801 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
     4861 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
     4921 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
     4981 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
     5041 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
     5101 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
     5161 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
     5221 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
     5281 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
     5341 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
     5401 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
     5461 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
     5521 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
     5581 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
     5641 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
     5701 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
     5761 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
     5821 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
     5881 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
     5941 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
     6001 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
     6061 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
     6121 gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg
     6181 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
     6241 ccgttattct aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac
     6301 actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctaagcttg
     6361 ggcccgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcta
     6421 tcaactttgt atagaaaagt tgtcatttag gtgacactat agaatactca agctatgcat
     6481 ccaacgcgtt gggagctctc ccatatggtc gactagagcc aagctgatct cctttgcccc
     6541 ggagatcacc atggacgact ttctctatct ctacgatcta ggaagaaagt tcgacggaga
     6601 aggtgacgat accatgttca ccaccgataa tgagaagatt agcctcttca atttcagaaa
     6661 gaatgctgac ccacagatgg ttagagaggc ctacgcggca ggtctcatca agacgatcta
     6721 cccgagtaat aatctccagg agatcaaata ccttcccaag aaggttaaag atgcagtcaa
     6781 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     6841 tactattcca gtatggacga ttcaaggctt gcttcataaa ccaaggcaag taatagagat
     6901 tggagtctct aagaaagtag ttcctactga atcaaaggcc atggagtcaa aaattcagat
     6961 cgaggatcta acagaactcg ccgtgaagac tggcgaacag ttcatacaga gtcttttacg
     7021 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacactc tcgtctactc
     7081 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
     7141 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag
     7201 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat
     7261 cgttcaagat gcccctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     7321 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     7381 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     7441 aggaagttca tttcatttgg agaggactcc ggtattttta caacaattac cacaacaaaa
     7501 caaacaacaa acaacattac aatttactat tctagtcgac ctgcaggcgg ccgcactagt
     7561 gatatcagca agtttgtaca aaaaagcagg ctcaatggtg agcaagggcg aggagctgtt
     7621 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     7681 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     7741 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     7801 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     7861 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     7921 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     7981 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     8041 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     8101 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     8161 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     8221 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     8281 gatcactctc ggcatggacg agctgtacaa ggacccagct ttcttgtaca aagtgggaga
     8341 caaggacaac accgtacccc tgaagctcat cgcactactt gctaatggtg aatttcactc
     8401 cggggaacag ctaggagaaa cactcggcat gtcaagagca gcaataaaca agcatataca
     8461 aacactccgt gactggggcg tggatgtatt taccgtccct ggtaagggat actccctccc
     8521 ggaaccaatc caacttctca atgccaagca gattctaggt caactcgatg gtggctcagt
     8581 ggcggtttta ccagtaatag actctacgaa ccagtatctg ctcgaccgaa taggggagct
     8641 taaatccgga gatgcttgca ttgcggaata tcaacaagcc ggacgtggtg gcaggggaag
     8701 gaagtggttc agtcccttcg gcgcgaatct ctatctgagc atgttctggc gacttgaaca
     8761 gggtcccgcg gccgctatcg gcctatcctt agttataggt atcgtcatgg ctgaggtcct
     8821 cagaaagcta ggggctgaca aggtgagggt aaaatggcct aatgacctct atcttcaaga
     8881 tagaaagttg gccggtatct tagtggaatt aacgggaaag accggcgatg ctgcacagat
     8941 cgtgatcggt gcgggaatta acatggccat gcgacgagtg gaagaaagcg ttgttaatca
     9001 gggatggatt acgctacaag aagccggtat aaatcttgac cgtaacaccc tagcagccat
     9061 gctgatccgt gaactacgag cggcgttgga actttttgaa caggagggtt tggcacctta
     9121 ccttagccga tgggaaaaac tcgacaactt cattaaccgt cccgtcaaat tgattattgg
     9181 cgataaggaa attttcggaa taagtagagg gatcgataag cagggagcgt tactacttga
     9241 gcaggatggt atcataaagc cgtggatggg aggcgaaatt tcactccgta gcgccgaaaa
     9301 ggcctaccca tacgatgttc cagattacgc ttacccatac gatgttccag attacgctta
     9361 cccatacgat gttccagatt acgcttgacc aactttatta tacatagttg ataattcact
     9421 ggccgtcgtt ttacaacgtc gtgactggga aaacgatcta actgactagc cgcggccatg
     9481 gcggccggga gcggccatgc tagagtccgc aaaaatcacc agtctctctc tacaaatcta
     9541 tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg gaattagggt
     9601 tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt
     9661 tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtgacctgca
     9721 ggtcgaccat atgggagagc tcgaattatc atacatgaga attaagggag tcacgttatg
     9781 acccccgccg atgacgcggg acaagccgtt ttacgtttgg aactgacaga accgcaacgt
     9841 tgaaggagcc actcagccgc gggtttctgg agtttaatga gctaagcaca tacgtcagaa
     9901 accattattg cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa tatttcttgt
     9961 caaaaatgct ccactgacgt tccataaatt cccctcggta tccaattaga gtctcatatt
    10021 cactctcaac tcgatcgagg catgattgaa caagatggat tgcacgcagg ttctccggcc
    10081 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
    10141 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
    10201 tccggtgccc tgaatgaact ccaagacgag gcagcgcggc tatcgtggct ggccacgacg
    10261 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
    10321 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
    10381 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
    10441 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
    10501 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
    10561 ctcaaggcgc ggatgcccga cggcgaggat ctcgtcgtga cccacggcga tgcctgcttg
    10621 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
    10681 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
    10741 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
    10801 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcggactct
    10861 agctagagtc aagcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
    10921 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
    10981 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
    11041 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
    11101 cgcgcggtgt catctatgtt actagatcga ccggcatgca agctgataat tcaattcggc
    11161 gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
    11221 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
    11281 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
    11341 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
    11401 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
    11461 cagagcgttg ctgcctgtga tca
//
