LOCUS       pEN-R2-LinkerTurboID-L3        4181 bp ds-DNA     circular     10-FEB-2023
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    pUC57.
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2020ï¿½ï¿½9ï¿½ï¿½11ï¿½ï¿½ from SnapGene 2.3.2
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     attR2           complement(1..126)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:kr-ezty4ze"">attR2</a>"
     Linker          128..322
                     /modified_by="mamee"
                     /label="linker"
     CDS             326..1288
                     /label="TurboID"
     tag             1292..1372
                     /modified_by="mamee"
                     /label="3xHA"
     attL3           complement(1377..1472)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lo-eztz06f"">attL3</a>"
     primer          complement(1530..1546)
                     /invitrogen=2010000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 reverse primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     reverse primer (pDONRP4-P3 - M13 reverse primer)"
                     /label="M13 reverse primer"
     rep_origin      complement(1932..2520)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     misc_feature    complement(2691..3551)
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="99.77%"
                     /Primary_Match="99.77%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     promoter        complement(3552..3656)
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     primer          4110..4126
                     /invitrogen=2000000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-40) forward primer (pDONRP4-P3 - M13 (-40) forward
                     primer)"
                     /label="M13 (-40) forward primer"
     primer          4130..4145
                     /invitrogen=1980000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-20) forward primer (pDONRP4-P3 - M13 (-20) forward
                     primer)"
                     /label="M13 (-20) forward primer"
ORIGIN      
        1 tcatagtgac tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
       61 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt tcttgtacaa
      121 agtgggaggt ggcggaggtt ctggcggtgg aggatccgga ggtggcgggt ccggtggtgg
      181 cggatcaggt ggaggaggtt cgggaggcgg aggtagtgga ggcggtggga gcggcggagg
      241 tgggagcggt ggcggaggga gtggaggtgg aggctcgggc ggaggtggct ccggcggagg
      301 tgggtctgga ggcggtggct cggacaaaga caataccgtg cccctcaaac taattgcatt
      361 gcttgccaat ggtgagttcc attctgggga acaacttggg gagactctgg gcatgtcccg
      421 agcggccatt aataagcaca tacagacttt gagggactgg ggtgtcgatg tgtttacagt
      481 accgggtaag ggatattctc tgccggagcc gataccttta ctcaatgcaa agcagatttt
      541 aggacagctg gatggcggga gcgtggctgt cctcccggtg gtcgatagta caaatcaata
      601 tcttttggac aggatcggtg agctgaaatc tggagatgct tgcatagcgg aataccaaca
      661 agctggtagg ggatctcgag gccgaaagtg gttttcaccc tttggggcca acttatactt
      721 atccatgttc tggcgtctga aacgtggccc ggcagccata gggctagggc ccgtaattgg
      781 gatcgttatg gcggaagcgt tacgaaagtt gggagccgat aaagtcagag taaagtggcc
      841 aaatgatctt tatttacaag atagaaagct ggcgggcatt cttgttgagc ttgccggaat
      901 aacaggcgat gccgcacaga tcgttatagg ggccgggatt aatgtggcta tgaggagggt
      961 cgaagaaagt gttgtgaatc aaggatggat tactctccaa gaggcgggta taaatcttga
     1021 tcgaaatact ctagctgcaa ccttgatacg agagctaaga gcagcactag aattgtttga
     1081 acaagaaggg ctggccccat atctccctcg atgggagaag ttggacaatt ttataaacag
     1141 accggtgaag ttaatcattg gagacaaaga aattttcggt ataagccgtg ggatcgataa
     1201 acagggtgct cttctccttg agcaggacgg tgtcatcaag ccttggatgg gaggcgaaat
     1261 ctctctccga agcgccgaga agaagcttgc ctacccatac gatgttccag attacgctta
     1321 cccatacgat gttccagatt acgcttaccc atacgatgtt ccagattacg cttgaccaac
     1381 tttattatac aaagttggca ttataaaaaa gcattgctta tcaatttgtt gcaacgaaca
     1441 ggtcactatc agtcaaaata aaatcattat ttatcggatc ccgggcccgt cgactgcaga
     1501 ggcctgcatg caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     1561 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     1621 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     1681 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     1741 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1801 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1861 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1921 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1981 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2041 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2101 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2161 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2221 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2281 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2341 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2401 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2461 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2521 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2581 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2641 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     2701 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     2761 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     2821 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     2881 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     2941 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3001 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3061 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3121 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3181 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3241 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3301 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3361 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3421 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3481 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3541 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3601 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     3661 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     3721 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     3781 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     3841 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     3901 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     3961 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
     4021 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     4081 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     4141 gccagtgaat tcgagctcgg tacctcgcga atgcatctag a
//
