LOCUS       pEN-R2-LinkerBioID2-L3        3175 bp    DNA     circular UNA 10-FEB-2023
DEFINITION  .
ACCESSION   urn.local...l0-citr5zy
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     attR2           complement(1..126)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:kr-ezty4ze"">attR2</a>"
     Linker          128..322
                     /modified_by="mamee"
                     /label="linker"
     CDS             326..1021
                     /label="BioID2"
     tag             1025..1105
                     /modified_by="mamee"
                     /label="3xHA"
     attL3           complement(1110..1205)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lo-eztz06f"">attL3</a>"
     misc_feature    1384..2244
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="99.07%"
                     /Primary_Match="99.07%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      complement(2499..3086)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.66%"
                     /Primary_Match="99.66%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
ORIGIN      
        1 tcatagtgac tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
       61 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt tcttgtacaa
      121 agtgggaggt ggcggaggtt ctggcggtgg aggatccgga ggtggcgggt ccggtggtgg
      181 cggatcaggt ggaggaggtt cgggaggcgg aggtagtgga ggcggtggga gcggcggagg
      241 tgggagcggt ggcggaggga gtggaggtgg aggctcgggc ggaggtggct ccggcggagg
      301 tgggtctgga ggcggtggct cggacttcaa aaacttaatt tggttaaaag aagtggattc
      361 aacacaggag cgacttaaag aatggaatgt aagttacggc acggcattag ttgccgatcg
      421 tcaaacgaag ggaagaggag ggttggggcg taagtggcta agtcaggaag gtgggctata
      481 cttcagtttt cttctgaatc cgaaggagtt cgagaacctg ctacaattac cgctcgtact
      541 gggcctgagt gtgagcgagg ctttagaaga aattacagaa atccccttca gtttgaagtg
      601 gccaaatgat gtctattttc aggaaaagaa agtctctggc gtactgtgcg aactatcaaa
      661 ggataagctg atcgtaggaa tcggtataaa tgttaatcaa cgagagatcc cggaggaaat
      721 caaagacaga gcaaccacgt tatatgagat taccgggaag gactgggata ggaaagaggt
      781 actattaaag gtactaaagc gaatatcaga aaatctcaag aaattcaagg aaaaaagctt
      841 caaagaattc aaaggcaaga ttgaaagcaa gatgctatac ctcggggaag aggttaagtt
      901 gcttggggaa ggaaaaatta ctggtaagct ggttggcctg agcgaaaagg gcggtgctct
      961 cattttaact gaggaaggga tcaaagaaat cttgagcgga gagttctcct tgcgtcgaag
     1021 cgcctaccca tacgatgttc cagattacgc ttacccatac gatgttccag attacgctta
     1081 cccatacgat gttccagatt acgcttgacc aactttatta tacaaagttg gcattataaa
     1141 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     1201 tatttacatc actgaccatt taaatcatac ctgacctcca tagcagaaag tcaaaagcct
     1261 ccgaccggag gcttttgact tgatcggcac gtaagaggtt ccaactttca ccataatgaa
     1321 ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct
     1381 aaaatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     1441 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1501 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagtttacgc
     1561 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1621 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1681 ttggttgagt actcaccagt cacagaaaag catctcacgg atggcatgac agtaagagaa
     1741 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctggcaacg
     1801 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1861 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1921 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1981 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg atcacttctg
     2041 cgctcggccc tcccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     2101 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgcat cgtagttatc
     2161 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     2221 gcctcactga ttaagcattg gtaagtgacc aaacaggaaa aaaccgccct taacatggcc
     2281 cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat
     2341 gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc
     2401 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgatgag ggcccaaatg taatcacctg
     2461 gctcaccttc gggtgggcct ttctgcgttg ctggcgtttt tccataggct ccgcccccct
     2521 gacgagcatc acaaaaatcg atgctcaagt cagaggtggc gaaacccgac aggactataa
     2581 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2641 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2701 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2761 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2821 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2881 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     2941 acagtatttg gtatctgcgc tctgctgaag ccagttacct cggaaaaaga gttggtagct
     3001 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3061 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat tttctaccga agaaaggccc
     3121 acccgtgaag gtgagccagt gagttgattg cagtccagtt acgctggagt caagc
//
