LOCUS       pEN-R2-BioID2-L3        2980 bp    DNA     circular UNA 10-FEB-2023
DEFINITION  .
ACCESSION   urn.local...ky-citr5zy
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     attR2           complement(1..126)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:kr-ezty4ze"">attR2</a>"
     CDS             131..826
                     /modified_by="mamee"
                     /label="BioID2"
     tag             830..910
                     /modified_by="mamee"
                     /label="3xHA"
     attL3           complement(915..1010)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lo-eztz06f"">attL3</a>"
     misc_feature    1189..2049
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="99.07%"
                     /Primary_Match="99.07%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      complement(2304..2891)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.66%"
                     /Primary_Match="99.66%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
ORIGIN      
        1 tcatagtgac tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
       61 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt tcttgtacaa
      121 agtgggagac ttcaaaaact taatttggtt aaaagaagtg gattcaacac aggagcgact
      181 taaagaatgg aatgtaagtt acggcacggc attagttgcc gatcgtcaaa cgaagggaag
      241 aggagggttg gggcgtaagt ggctaagtca ggaaggtggg ctatacttca gttttcttct
      301 gaatccgaag gagttcgaga acctgctaca attaccgctc gtactgggcc tgagtgtgag
      361 cgaggcttta gaagaaatta cagaaatccc cttcagtttg aagtggccaa atgatgtcta
      421 ttttcaggaa aagaaagtct ctggcgtact gtgcgaacta tcaaaggata agctgatcgt
      481 aggaatcggt ataaatgtta atcaacgaga gatcccggag gaaatcaaag acagagcaac
      541 cacgttatat gagattaccg ggaaggactg ggataggaaa gaggtactat taaaggtact
      601 aaagcgaata tcagaaaatc tcaagaaatt caaggaaaaa agcttcaaag aattcaaagg
      661 caagattgaa agcaagatgc tatacctcgg ggaagaggtt aagttgcttg gggaaggaaa
      721 aattactggt aagctggttg gcctgagcga aaagggcggt gctctcattt taactgagga
      781 agggatcaaa gaaatcttga gcggagagtt ctccttgcgt cgaagcgcct acccatacga
      841 tgttccagat tacgcttacc catacgatgt tccagattac gcttacccat acgatgttcc
      901 agattacgct tgaccaactt tattatacaa agttggcatt ataaaaaagc attgcttatc
      961 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt acatcactga
     1021 ccatttaaat catacctgac ctccatagca gaaagtcaaa agcctccgac cggaggcttt
     1081 tgacttgatc ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg
     1141 ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat gagtattcaa
     1201 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     1261 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     1321 atcgaactgg atctcaacag cggtaagatc cttgagagtt tacgccccga agaacgtttt
     1381 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     1441 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     1501 ccagtcacag aaaagcatct cacggatggc atgacagtaa gagaattatg cagtgctgcc
     1561 ataaccatga gtgataacac tgcggccaac ttacttctgg caacgatcgg aggaccgaag
     1621 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1681 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1741 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1801 ttaatagact ggatggaggc ggataaagtt gcaggatcac ttctgcgctc ggccctcccg
     1861 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1921 gcagcactgg ggccagatgg taagccctcc cgcatcgtag ttatctacac gacggggagt
     1981 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2041 cattggtaag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc
     2101 cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc
     2161 tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt
     2221 gatgacggtg aaaacctctg atgagggccc aaatgtaatc acctggctca ccttcgggtg
     2281 ggcctttctg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2341 aatcgatgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2401 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2461 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2521 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2581 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2641 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2701 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2761 tgcgctctgc tgaagccagt tacctcggaa aaagagttgg tagctcttga tccggcaaac
     2821 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2881 aaggatctca agaagatcct ttgattttct accgaagaaa ggcccacccg tgaaggtgag
     2941 ccagtgagtt gattgcagtc cagttacgct ggagtcaagc
//
