LOCUS       pEN-R2-BioID-L3         3244 bp    DNA     circular UNA 10-FEB-2023
DEFINITION  .
ACCESSION   urn.local...kx-citr5zw
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     attR2           complement(1..126)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:kr-ezty4ze"">attR2</a>"
     CDS             131..1090
                     /label="BioID"
     tag             1094..1174
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="3xHA"
     attL3           complement(1179..1274)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lo-eztz06f"">attL3</a>"
     misc_feature    1453..2313
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="99.07%"
                     /Primary_Match="99.07%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      complement(2568..3155)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.66%"
                     /Primary_Match="99.66%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
ORIGIN      
        1 tcatagtgac tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
       61 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt tcttgtacaa
      121 agtgggagac aaggacaaca ccgtacccct gaagctcatc gcactacttg ctaatggtga
      181 atttcactcc ggggaacagc taggagaaac actcggcatg tcaagagcag caataaacaa
      241 gcatatacaa acactccgtg actggggcgt ggatgtattt accgtccctg gtaagggata
      301 ctccctcccg gaaccaatcc aacttctcaa tgccaagcag attctaggtc aactcgatgg
      361 tggctcagtg gcggttttac cagtaataga ctctacgaac cagtatctgc tcgaccgaat
      421 aggggagctt aaatccggag atgcttgcat tgcggaatat caacaagccg gacgtggtgg
      481 caggggaagg aagtggttca gtcccttcgg cgcgaatctc tatctgagca tgttctggcg
      541 acttgaacag ggtcccgcgg ccgctatcgg cctatcctta gttataggta tcgtcatggc
      601 tgaggtcctc agaaagctag gggctgacaa ggtgagggta aaatggccta atgacctcta
      661 tcttcaagat agaaagttgg ccggtatctt agtggaatta acgggaaaga ccggcgatgc
      721 tgcacagatc gtgatcggtg cgggaattaa catggccatg cgacgagtgg aagaaagcgt
      781 tgttaatcag ggatggatta cgctacaaga agccggtata aatcttgacc gtaacaccct
      841 agcagccatg ctgatccgtg aactacgagc ggcgttggaa ctttttgaac aggagggttt
      901 ggcaccttac cttagccgat gggaaaaact cgacaacttc attaaccgtc ccgtcaaatt
      961 gattattggc gataaggaaa ttttcggaat aagtagaggg atcgataagc agggagcgtt
     1021 actacttgag caggatggta tcataaagcc gtggatggga ggcgaaattt cactccgtag
     1081 cgccgaaaag gcctacccat acgatgttcc agattacgct tacccatacg atgttccaga
     1141 ttacgcttac ccatacgatg ttccagatta cgcttgacca actttattat acaaagttgg
     1201 cattataaaa aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     1261 taaaatcatt atttacatca ctgaccattt aaatcatacc tgacctccat agcagaaagt
     1321 caaaagcctc cgaccggagg cttttgactt gatcggcacg taagaggttc caactttcac
     1381 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     1441 aaggaagcta aaatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     1501 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     1561 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     1621 agtttacgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     1681 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     1741 cagaatgact tggttgagta ctcaccagtc acagaaaagc atctcacgga tggcatgaca
     1801 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     1861 ctggcaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     1921 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     1981 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     2041 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     2101 tcacttctgc gctcggccct cccggctggc tggtttattg ctgataaatc tggagccggt
     2161 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgcatc
     2221 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     2281 gagataggtg cctcactgat taagcattgg taagtgacca aacaggaaaa aaccgccctt
     2341 aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg
     2401 gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac
     2461 cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgatgagg gcccaaatgt
     2521 aatcacctgg ctcaccttcg ggtgggcctt tctgcgttgc tggcgttttt ccataggctc
     2581 cgcccccctg acgagcatca caaaaatcga tgctcaagtc agaggtggcg aaacccgaca
     2641 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2701 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2761 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2821 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2881 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2941 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3001 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctc ggaaaaagag
     3061 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3121 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatt ttctaccgaa
     3181 gaaaggccca cccgtgaagg tgagccagtg agttgattgc agtccagtta cgctggagtc
     3241 aagc
//
