LOCUS       pCambia-p35s-TurboID-GFP        9517 bp    DNA     circular UNA 20-MAR-2023
DEFINITION  .
ACCESSION   urn.local...kw-citr5zw
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..835
                     /modified_by="mamee"
                     /label="p35S"
     CDS             848..1828
                     /feature_type="Insertion"
                     /origin="GOI-Turbo ID (1,007bp linear)Fragment: from
                     BsaIcut at 11^12 to BsaI cut at 996^997,
                     985bpOrientation:originalPlus strand 5': no changePlus
                     strand 3': nochangeMinus strand 5': no changeMinus strand
                     3': nochange"
                     /modified_by="mamee"
                     /label="TurboID"
     Linker          1835..1879
                     /modified_by="mamee"
                     /label="15 aa linker"
     CDS             1880..2596
                     /gene="sGFP"
                     /note="sGFP (S65T) gene"
                     /codon_start=1
                     /transl_table=11
                     /product="sGFP"
                     /protein_id="BAG12994.1"
                     /db_xref="GI:170172452"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITHGMDELYK"
                     /modified_by="mamee"
                     /label="sGFP"
     terminator      2597..2826
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="35ST"
                     /Transferred_Similarity="95.00%"
                     /Primary_Match="95.00%; Adjusted-Interval-0:0/0; t35S (<a
                     href=""urn:local:.:6d-dgycryw"">35ST</a>)"
                     /label="t35S"
     regulatory      4212..4841
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     regulatory      5270..6343
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     rep_origin      6409..6603
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     regulatory      6977..7237
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150;
                     <a href=""urn:local:.:79-dlucaql"">bom</a>"
                     /label="pBR322 bom"
     rep_origin      complement(7273..7861)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     Promoter        8009..8283
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    8284..9075
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
ORIGIN      
        1 agattagcct tttcaatttc agaaagaatg ctaacccaca gatggttaga gaggcttacg
       61 cagcacgact catcaagacg atctacccga gcaataatct ccaggaaatc aaataccttc
      121 ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga
      181 aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc
      241 acaaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttccc actgaatcaa
      301 aggccatgga gtcaaagatt caaatagagg acctaacaga actcgccgta aagactggcg
      361 aacagttcat acagagtctc ttacgactca atgacaagaa gaaaatcttc gtcaacatgg
      421 tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagagcaaa
      481 gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc
      541 cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc
      601 atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag
      661 atggaccccc acccacgagg agcatcgtgg aaaaagaaca cgttccaacc acgacttcaa
      721 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc
      781 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagaga acacgtctgg
      841 aagcaccatg aaagacaata ctgtgcctct gaagctgatc gctctcctgg ctaatggcga
      901 gttccatagt ggcgaacagc tgggagaaac cctgggcatg tccagggccg ctatcaacaa
      961 gcacattcag actctgcgcg actggggcgt ggacgtgttc accgtgcccg gaaagggcta
     1021 ctctctgccc gagcctatcc cgctgctgaa cgctaaacag attctgggac agctggacgg
     1081 cgggagcgtg gcagtcctgc ctgtggtcga ctccaccaat cagtacctgc tggatcgaat
     1141 cggcgagctg aagagtgggg atgcttgcat tgcagaatat cagcaggcag ggagaggaag
     1201 cagagggagg aaatggttct ctccttttgg agctaacctg tacctgagta tgttttggcg
     1261 cctgaagcgg ggaccagcag caatcggcct gggcccggtc atcggaattg tcatggcaga
     1321 agcgctgcga aagctgggag cagacaaggt gcgagtcaaa tggcccaatg acctgtatct
     1381 gcaggataga aagctggcag gcatcctggt ggagctggcc ggaataacag gcgatgctgc
     1441 acagatcgtc attggcgccg ggattaacgt ggctatgagg cgcgtggagg aaagcgtggt
     1501 caatcagggc tggatcacac tgcaggaagc agggattaac ctggacagga atactctggc
     1561 cgctacgctg atccgagagc tgcgggcagc cctggaactg ttcgagcagg aaggcctggc
     1621 tccatatctg ccacggtggg agaagctgga taacttcatc aatagacccg tgaagctgat
     1681 cattggggac aaagagattt tcgggattag ccgggggatt gataaacagg gagccctgct
     1741 gctggaacag gacggagtta tcaaaccctg gatgggcgga gaaatcagtc tgcggtctgc
     1801 cgaaaagaag cttgcggccg cactcgagaa gggaggtgga ggaggttctg gaggcggtgg
     1861 aagtggtggc ggaggtagcg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     1921 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     1981 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     2041 cgtgccctgg cccaccctcg tgaccacctt cacctacggc gtgcagtgct tcagccgcta
     2101 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     2161 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     2221 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     2281 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     2341 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     2401 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     2461 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     2521 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc acggcatgga
     2581 cgagctgtac aagtaaaatc cggccatgct agagtccgca aaaatcacca gtctctctct
     2641 acaaatctat ctctctctat ttttctccag aataatgtgt gagtagttcc cagataaggg
     2701 aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt
     2761 atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag
     2821 tgaccttgag tgacctaagc tagcctagtg tcgggcttgt cttctctggt ttaaactatc
     2881 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
     2941 tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca
     3001 accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc
     3061 agtcggcttc tgacgttcag tgcagccttc ttctgaaaac gacatgtcgc acaagtccta
     3121 agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag
     3181 tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc
     3241 cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca
     3301 acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac
     3361 caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt
     3421 gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga
     3481 gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac
     3541 cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc
     3601 cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt
     3661 tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca
     3721 ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta
     3781 ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt
     3841 ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga
     3901 ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag
     3961 atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca catctcaacc
     4021 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc
     4081 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa
     4141 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc
     4201 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga
     4261 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg
     4321 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa
     4381 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg
     4441 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg
     4501 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg
     4561 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct
     4621 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg
     4681 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca
     4741 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg
     4801 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa
     4861 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc
     4921 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc
     4981 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga
     5041 gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta
     5101 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg
     5161 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg
     5221 cctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg
     5281 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
     5341 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
     5401 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
     5461 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
     5521 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
     5581 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
     5641 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
     5701 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
     5761 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
     5821 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
     5881 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
     5941 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
     6001 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
     6061 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
     6121 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
     6181 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
     6241 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
     6301 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
     6361 agggcaaatt gccctagcag gggaaaaagg tcgaaaagga ctctttcctg tggatagcac
     6421 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
     6481 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
     6541 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
     6601 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
     6661 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
     6721 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
     6781 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     6841 tctgacacat gcagctcccg gagacagtca cagcttgtct gtaagcggat gccgggagca
     6901 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     6961 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     7021 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     7081 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7141 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7201 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7261 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7321 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7381 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7441 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7501 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7561 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7621 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7681 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     7741 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7801 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7861 agaagatcct ttgatctttt ctacggggtc tgacgctcat cggaagaacg gcaactaagc
     7921 tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca
     7981 gagcgttgct gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt
     8041 cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     8101 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg
     8161 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     8221 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     8281 atcatggggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     8341 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     8401 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
     8461 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     8521 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     8581 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     8641 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     8701 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     8761 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     8821 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     8881 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     8941 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     9001 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
     9061 gtagtcggca aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg
     9121 gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc
     9181 aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata
     9241 tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9301 gaaaagatca aaggatcttc tgccggtaac atgagcaaag tctgccgcct tacaacggct
     9361 ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg
     9421 agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa
     9481 ttgacgctta gacggattac ggaagacaat acggcgg
//
