LOCUS       pGG-D-TurboID-HIS-E        3489 bp    DNA     circular UNA 10-FEB-2023
DEFINITION  .
ACCESSION   urn.local...18-de9m5yj
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="D*"
     CDS             14..994
                     /label="TurboID"
     tag             995..1012
                     /modified_by="mamee"
                     /label="6xHIS"
     GG              complement(1013..1023)
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="E*"
     primer          complement(1055..1071)
                     /invitrogen=2010000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 reverse primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     reverse primer (pDONRP4-P3 - M13 reverse primer)"
                     /label="M13 reverse primer"
     rep_origin      complement(1457..2045)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     misc_feature    2377..2910
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="GmR"
                     /Transferred_Similarity="99.81%"
                     /Primary_Match="99.81%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:4q-cj2wxsi"">GmR</a>"
                     /label="GmR"
                     /note="Geneious type: Bacterial Selection"
     primer          3383..3399
                     /invitrogen=2000000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-40) forward primer (pDONRP4-P3 - M13 (-40) forward
                     primer)"
                     /label="M13 (-40) forward primer"
     primer          3403..3418
                     /invitrogen=1980000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-20) forward primer (pDONRP4-P3 - M13 (-20) forward
                     primer)"
                     /label="M13 (-20) forward primer"
ORIGIN      
        1 ggtctcaaag ggaatgaaag acaatactgt gcctctgaag ctgatcgctc tcctggctaa
       61 tggcgagttc catagtggcg aacagctggg agaaaccctg ggcatgtcca gggccgctat
      121 caacaagcac attcagactc tgcgcgactg gggcgtggac gtgttcaccg tgcccggaaa
      181 gggctactct ctgcccgagc ctatcccgct gctgaacgct aaacagattc tgggacagct
      241 ggacggcggg agcgtggcag tcctgcctgt ggtcgactcc accaatcagt acctgctgga
      301 tcgaatcggc gagctgaaga gtggggatgc ttgcattgca gaatatcagc aggcagggag
      361 aggaagcaga gggaggaaat ggttctctcc ttttggagct aacctgtacc tgagtatgtt
      421 ttggcgcctg aagcggggac cagcagcaat cggcctgggc ccggtcatcg gaattgtcat
      481 ggcagaagcg ctgcgaaagc tgggagcaga caaggtgcga gtcaaatggc ccaatgacct
      541 gtatctgcag gatagaaagc tggcaggcat cctggtggag ctggccggaa taacaggcga
      601 tgctgcacag atcgtcattg gcgccgggat taacgtggct atgaggcgcg tggaggaaag
      661 cgtggtcaat cagggctgga tcacactgca ggaagcaggg attaacctgg acaggaatac
      721 tctggccgct acgctgatcc gagagctgcg ggcagccctg gaactgttcg agcaggaagg
      781 cctggctcca tatctgccac ggtgggagaa gctggataac ttcatcaata gacccgtgaa
      841 gctgatcatt ggggacaaag agattttcgg gattagccgg gggattgata aacagggagc
      901 cctgctgctg gaacaggacg gagttatcaa accctggatg ggcggagaaa tcagtctgcg
      961 gtctgccgaa aagaagcttg cggccgcact cgagcaccac caccaccacc acaatctgag
     1021 acctctgcct gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     1081 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     1141 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     1201 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     1261 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1321 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1381 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1441 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1501 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1561 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1621 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1681 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1741 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1801 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1861 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1921 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1981 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     2041 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     2101 gttaagggat tttggtcatg aggcgcacac cgtggaaacg gatgaaggca cgaacccagt
     2161 tgacataagc ctgttcggtt cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact
     2221 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     2281 gttatgactg tttttttgta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2341 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag caacgatgtt
     2401 acgcagcagg gcagtcgccc taaaacaaag ttaggtggct caagtatggg catcattcgc
     2461 acatgtaggc tcggccctga ccaagtcaaa tccatgcggg ctgctcttga tcttttcggt
     2521 cgtgagttcg gcgacgtagc cacctactcc caacatcagc cggactccga ttacctcggg
     2581 aacttgctcc gtagtaagac attcatcgcg cttgctgcct tcgaccaaga agcggttgtt
     2641 ggcgctctcg cggcttacgt tctgcccagg tttgagcagc cgcgtagtga gatctatatc
     2701 tatgatctcg cagtctccgg cgagcaccgg aggcagggca ttgccaccgc gctcatcaat
     2761 ctcctcaagc atgaggccaa cgcgcttggt gcttatgtga tctacgtgca agcagattac
     2821 ggtgacgatc ccgcagtggc tctctataca aagttgggca tacgggaaga agtgatgcac
     2881 tttgatatcg acccaagtac cgccacctaa caattcgttc aagccgagat cggcttcccg
     2941 gccgcggagt tgttcggtaa attgtcacaa cgccgctcat gacattaacc tataaaaata
     3001 ggcgtatcac gaggcccttt tgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     3061 acatgcagct cccggagaag gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     3121 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     3181 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     3241 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     3301 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     3361 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     3421 aattcgagct cggtacctcg cgaatgcatc tagatatcgg atcccgggcc cgtcgactgc
     3481 agaggtacg
//
