LOCUS       pGG-D-TurboID-E         3474 bp    DNA     circular UNA 10-FEB-2023
DEFINITION  .
ACCESSION   urn.local...14-de9m5yg
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="D*"
     CDS             14..997
                     /modified_by="mamee"
                     /label="TurboID*"
     GG              complement(998..1008)
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="E*"
     primer          complement(1040..1056)
                     /invitrogen=2010000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 reverse primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     reverse primer (pDONRP4-P3 - M13 reverse primer)"
                     /label="M13 reverse primer"
     rep_origin      complement(1442..2030)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     misc_feature    2362..2895
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="GmR"
                     /Transferred_Similarity="99.81%"
                     /Primary_Match="99.81%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:4q-cj2wxsi"">GmR</a>"
                     /label="GmR"
                     /note="Geneious type: Bacterial Selection"
     primer          3368..3384
                     /invitrogen=2000000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-40) forward primer (pDONRP4-P3 - M13 (-40) forward
                     primer)"
                     /label="M13 (-40) forward primer"
     primer          3388..3403
                     /invitrogen=1980000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-20) forward primer (pDONRP4-P3 - M13 (-20) forward
                     primer)"
                     /label="M13 (-20) forward primer"
ORIGIN      
        1 ggtctcaaag ggaatgaaag acaatactgt gcctctgaag ctgatcgctc tcctggctaa
       61 tggcgagttc catagtggcg aacagctggg agaaaccctg ggcatgtcca gggccgctat
      121 caacaagcac attcagactc tgcgcgactg gggcgtggac gtgttcaccg tgcccggaaa
      181 gggctactct ctgcccgagc ctatcccgct gctgaacgct aaacagattc tgggacagct
      241 ggacggcggg agcgtggcag tcctgcctgt ggtcgactcc accaatcagt acctgctgga
      301 tcgaatcggc gagctgaaga gtggggatgc ttgcattgca gaatatcagc aggcagggag
      361 aggaagcaga gggaggaaat ggttctctcc ttttggagct aacctgtacc tgagtatgtt
      421 ttggcgcctg aagcggggac cagcagcaat cggcctgggc ccggtcatcg gaattgtcat
      481 ggcagaagcg ctgcgaaagc tgggagcaga caaggtgcga gtcaaatggc ccaatgacct
      541 gtatctgcag gatagaaagc tggcaggcat cctggtggag ctggccggaa taacaggcga
      601 tgctgcacag atcgtcattg gcgccgggat taacgtggct atgaggcgcg tggaggaaag
      661 cgtggtcaat cagggctgga tcacactgca ggaagcaggg attaacctgg acaggaatac
      721 tctggccgct acgctgatcc gagagctgcg ggcagccctg gaactgttcg agcaggaagg
      781 cctggctcca tatctgccac ggtgggagaa gctggataac ttcatcaata gacccgtgaa
      841 gctgatcatt ggggacaaag agattttcgg gattagccgg gggattgata aacagggagc
      901 cctgctgctg gaacaggacg gagttatcaa accctggatg ggcggagaaa tcagtctgcg
      961 gtctgccgaa aagaagcttg cggccgcact cgagtagaat ctgagacctc tgcctgcatg
     1021 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     1081 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     1141 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     1201 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     1261 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1321 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1381 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1441 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1501 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1561 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1621 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1681 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1741 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1801 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1861 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1921 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1981 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2041 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2101 tcatgaggcg cacaccgtgg aaacggatga aggcacgaac ccagttgaca taagcctgtt
     2161 cggttcgtaa actgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     2221 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     2281 ttgtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     2341 tgatgttatg gagcagcaac gatgttacgc agcagcaacg atgttacgca gcagggcagt
     2401 cgccctaaaa caaagttagg tggctcaagt atgggcatca ttcgcacatg taggctcggc
     2461 cctgaccaag tcaaatccat gcgggctgct cttgatcttt tcggtcgtga gttcggcgac
     2521 gtagccacct actcccaaca tcagccggac tccgattacc tcgggaactt gctccgtagt
     2581 aagacattca tcgcgcttgc tgccttcgac caagaagcgg ttgttggcgc tctcgcggct
     2641 tacgttctgc ccaggtttga gcagccgcgt agtgagatct atatctatga tctcgcagtc
     2701 tccggcgagc accggaggca gggcattgcc accgcgctca tcaatctcct caagcatgag
     2761 gccaacgcgc ttggtgctta tgtgatctac gtgcaagcag attacggtga cgatcccgca
     2821 gtggctctct atacaaagtt gggcatacgg gaagaagtga tgcactttga tatcgaccca
     2881 agtaccgcca cctaacaatt cgttcaagcc gagatcggct tcccggccgc ggagttgttc
     2941 ggtaaattgt cacaacgccg ctcatgacat taacctataa aaataggcgt atcacgaggc
     3001 ccttttgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     3061 agaaggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     3121 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     3181 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     3241 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     3301 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     3361 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcggta
     3421 cctcgcgaat gcatctagat atcggatccc gggcccgtcg actgcagagg tacg
//
