LOCUS       pGG-B-TurboID-C         3475 bp    DNA     circular UNA 10-FEB-2023
DEFINITION  .
ACCESSION   urn.local...o-de9m5y4
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="B*"
     CDS             16..996
                     /label="TurboID"
     GG              complement(999..1009)
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="C*"
     primer          complement(1041..1057)
                     /invitrogen=2010000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 reverse primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     reverse primer (pDONRP4-P3 - M13 reverse primer)"
                     /label="M13 reverse primer"
     primer          complement(1058..1079)
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
     rep_origin      complement(1443..2031)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     misc_feature    2363..2896
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="GmR"
                     /Transferred_Similarity="99.81%"
                     /Primary_Match="99.81%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:4q-cj2wxsi"">GmR</a>"
                     /label="GmR"
                     /note="Geneious type: Bacterial Selection"
     primer          3369..3385
                     /invitrogen=2000000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-40) forward primer (pDONRP4-P3 - M13 (-40) forward
                     primer)"
                     /label="M13 (-40) forward primer"
     primer          3389..3404
                     /invitrogen=1980000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-20) forward primer (pDONRP4-P3 - M13 (-20) forward
                     primer)"
                     /label="M13 (-20) forward primer"
ORIGIN      
        1 ggtctcatct gaacaatgaa agacaatact gtgcctctga agctgatcgc tctcctggct
       61 aatggcgagt tccatagtgg cgaacagctg ggagaaaccc tgggcatgtc cagggccgct
      121 atcaacaagc acattcagac tctgcgcgac tggggcgtgg acgtgttcac cgtgcccgga
      181 aagggctact ctctgcccga gcctatcccg ctgctgaacg ctaaacagat tctgggacag
      241 ctggacggcg ggagcgtggc agtcctgcct gtggtcgact ccaccaatca gtacctgctg
      301 gatcgaatcg gcgagctgaa gagtggggat gcttgcattg cagaatatca gcaggcaggg
      361 agaggaagca gagggaggaa atggttctct ccttttggag ctaacctgta cctgagtatg
      421 ttttggcgcc tgaagcgggg accagcagca atcggcctgg gcccggtcat cggaattgtc
      481 atggcagaag cgctgcgaaa gctgggagca gacaaggtgc gagtcaaatg gcccaatgac
      541 ctgtatctgc aggatagaaa gctggcaggc atcctggtgg agctggccgg aataacaggc
      601 gatgctgcac agatcgtcat tggcgccggg attaacgtgg ctatgaggcg cgtggaggaa
      661 agcgtggtca atcagggctg gatcacactg caggaagcag ggattaacct ggacaggaat
      721 actctggccg ctacgctgat ccgagagctg cgggcagccc tggaactgtt cgagcaggaa
      781 ggcctggctc catatctgcc acggtgggag aagctggata acttcatcaa tagacccgtg
      841 aagctgatca ttggggacaa agagattttc gggattagcc gggggattga taaacaggga
      901 gccctgctgc tggaacagga cggagttatc aaaccctgga tgggcggaga aatcagtctg
      961 cggtctgccg aaaagaagct tgcggccgca ctcgagggca cctgagacct ctgcctgcat
     1021 gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     1081 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     1141 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     1201 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     1261 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1321 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     1381 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1441 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1501 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1561 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1621 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1681 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1741 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1801 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1861 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1921 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1981 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2041 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2101 gtcatgaggc gcacaccgtg gaaacggatg aaggcacgaa cccagttgac ataagcctgt
     2161 tcggttcgta aactgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
     2221 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     2281 tttgtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
     2341 ttgatgttat ggagcagcaa cgatgttacg cagcagcaac gatgttacgc agcagggcag
     2401 tcgccctaaa acaaagttag gtggctcaag tatgggcatc attcgcacat gtaggctcgg
     2461 ccctgaccaa gtcaaatcca tgcgggctgc tcttgatctt ttcggtcgtg agttcggcga
     2521 cgtagccacc tactcccaac atcagccgga ctccgattac ctcgggaact tgctccgtag
     2581 taagacattc atcgcgcttg ctgccttcga ccaagaagcg gttgttggcg ctctcgcggc
     2641 ttacgttctg cccaggtttg agcagccgcg tagtgagatc tatatctatg atctcgcagt
     2701 ctccggcgag caccggaggc agggcattgc caccgcgctc atcaatctcc tcaagcatga
     2761 ggccaacgcg cttggtgctt atgtgatcta cgtgcaagca gattacggtg acgatcccgc
     2821 agtggctctc tatacaaagt tgggcatacg ggaagaagtg atgcactttg atatcgaccc
     2881 aagtaccgcc acctaacaat tcgttcaagc cgagatcggc ttcccggccg cggagttgtt
     2941 cggtaaattg tcacaacgcc gctcatgaca ttaacctata aaaataggcg tatcacgagg
     3001 cccttttgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     3061 gagaaggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     3121 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     3181 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     3241 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     3301 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     3361 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt
     3421 acctcgcgaa tgcatctaga tatcggatcc cgggcccgtc gactgcagag gtacg
//
