LOCUS       pGG-D-linker-TurboID-HA-E        3888 bp ds-DNA     circular SYN 23-FEB-2023
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    KF718967- pGGD000.
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU and
            Forner J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
REFERENCE   3  (bases 1 to 3749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 11, 2018 from SnapGene Viewer 3.2.1
            http://www.snapgene.com
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:89-dgycryy"">D</a>"
                     /label="D"
     Linker          20..214
                     /cds_type="ORF"
                     /modified_by="mamee"
                     /label="13xGly4Ser"
     CDS             218..1180
                     /label="TurboID"
     tag             1184..1210
                     /cds_type="ORF"
                     /modified_by="mamee"
                     /label="1xHA"
     GG              complement(1214..1224)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="E"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:82-dgycryx"">E</a>"
                     /label="E"
     primer          complement(1244..1264)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1744..1848
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1849..2709
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2880..3468
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3832..3853
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcatca gcagtgggag gtggcggagg ttctggcggt ggaggatccg gaggtggcgg
       61 gtccggtggt ggcggatcag gtggaggagg ttcgggaggc ggaggtagtg gaggcggtgg
      121 gagcggcgga ggtgggagcg gtggcggagg gagtggaggt ggaggctcgg gcggaggtgg
      181 ctccggcgga ggtgggtctg gaggcggtgg ctcggacaaa gacaataccg tgcccctcaa
      241 actaattgca ttgcttgcca atggtgagtt ccattctggg gaacaacttg gggagactct
      301 gggcatgtcc cgagcggcca ttaataagca catacagact ttgagggact ggggtgtcga
      361 tgtgtttaca gtaccgggta agggatattc tctgccggag ccgatacctt tactcaatgc
      421 aaagcagatt ttaggacagc tggatggcgg gagcgtggct gtcctcccgg tggtcgatag
      481 tacaaatcaa tatcttttgg acaggatcgg tgagctgaaa tctggagatg cttgcatagc
      541 ggaataccaa caagctggta ggggatctcg aggccgaaag tggttttcac cctttggggc
      601 caacttatac ttatccatgt tctggcgtct gaaacgtggc ccggcagcca tagggctagg
      661 gcccgtaatt gggatcgtta tggcggaagc gttacgaaag ttgggagccg ataaagtcag
      721 agtaaagtgg ccaaatgatc tttatttaca agatagaaag ctggcgggca ttcttgttga
      781 gcttgccgga ataacaggcg atgccgcaca gatcgttata ggggccggga ttaatgtggc
      841 tatgaggagg gtcgaagaaa gtgttgtgaa tcaaggatgg attactctcc aagaggcggg
      901 tataaatctt gatcgaaata ctctagctgc aaccttgata cgagagctaa gagcagcact
      961 agaattgttt gaacaagaag ggctggcccc atatctccct cgatgggaga agttggacaa
     1021 ttttataaac agaccggtga agttaatcat tggagacaaa gaaattttcg gtataagccg
     1081 tgggatcgat aaacagggtg ctcttctcct tgagcaggac ggtgtcatca agccttggat
     1141 gggaggcgaa atctctctcc gaagcgccga gaagaagctt gcctacccat acgatgttcc
     1201 agattacgct tagctgctga gaccgaattc tcgccctata gtgagtcgta ttacctggcc
     1261 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     1321 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     1381 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
     1441 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     1501 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     1561 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     1621 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     1681 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     1741 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1801 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     1861 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     1921 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     1981 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     2041 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     2101 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     2161 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2221 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     2281 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2341 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     2401 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2461 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2521 gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg aggtatcatt
     2581 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2641 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2701 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2761 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     2821 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2881 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2941 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3001 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3061 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3121 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3181 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3241 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3301 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3361 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3421 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3481 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3541 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3601 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3661 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3721 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     3781 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     3841 taacaatttc acacgagcta tttaggtgac actatagaag tgaagctt
//
