LOCUS       pGG-B-HA-TurboID-linker-C        3978 bp    DNA     circular SYN 23-FEB-2023
DEFINITION  .
ACCESSION   KF718965
VERSION     KF718965.1
KEYWORDS    .
SOURCE      Cloning vector pGGB000 (unknown)
  ORGANISM  Cloning vector pGGB000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:87-dgycryy"">B</a>"
                     /label="B"
     misc_difference 12..43
                     /label="omega leader"
     tag             47..127
                     /cds_type="ORF"
                     /modified_by="mamee"
                     /label="3xHA"
     CDS             131..1093
                     /cds_type="ORF"
                     /label="TurboID"
     Linker          1097..1291
                     /cds_type="ORF"
                     /modified_by="mamee"
                     /label="13xG4Slinker"
     GG              complement(1304..1314)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:80-dgycryx"">C</a>"
                     /label="C"
     primer          complement(1334..1354)
                     /label="GG_SEQ_rev"
     promoter        1834..1938
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1939..2799
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2970..3558
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3922..3943
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaaac aaacaacaaa caacaaacaa cattacaatt acaatgtacc catacgatgt
       61 tccagattac gcttacccat acgatgttcc agattacgct tacccatacg atgttccaga
      121 ttacgctgcc aaagacaata ccgtgcccct caaactaatt gcattgcttg ccaatggtga
      181 gttccattct ggggaacaac ttggggagac tctgggcatg tcccgagcgg ccattaataa
      241 gcacatacag actttgaggg actggggtgt cgatgtgttt acagtaccgg gtaagggata
      301 ttctctgccg gagccgatac ctttactcaa tgcaaagcag attttaggac agctggatgg
      361 cgggagcgtg gctgtcctcc cggtggtcga tagtacaaat caatatcttt tggacaggat
      421 cggtgagctg aaatctggag atgcttgcat agcggaatac caacaagctg gtaggggatc
      481 tcgaggccga aagtggtttt caccctttgg ggccaactta tacttatcca tgttctggcg
      541 tctgaaacgt ggcccggcag ccatagggct agggcccgta attgggatcg ttatggcgga
      601 agcgttacga aagttgggag ccgataaagt cagagtaaag tggccaaatg atctttattt
      661 acaagataga aagctggcgg gcattcttgt tgagcttgcc ggaataacag gcgatgccgc
      721 acagatcgtt ataggggccg ggattaatgt ggctatgagg agggtcgaag aaagtgttgt
      781 gaatcaagga tggattactc tccaagaggc gggtataaat cttgatcgaa atactctagc
      841 tgcaaccttg atacgagagc taagagcagc actagaattg tttgaacaag aagggctggc
      901 cccatatctc cctcgatggg agaagttgga caattttata aacagaccgg tgaagttaat
      961 cattggagac aaagaaattt tcggtataag ccgtgggatc gataaacagg gtgctcttct
     1021 ccttgagcag gacggtgtca tcaagccttg gatgggaggc gaaatctctc tccgaagcgc
     1081 cgagaagaag cttgacggtg gcggaggttc tggcggtgga ggatccggag gtggcgggtc
     1141 cggtggtggc ggatcaggtg gaggaggttc gggaggcgga ggtagtggag gcggtgggag
     1201 cggcggaggt gggagcggtg gcggagggag tggaggtgga ggctcgggcg gaggtggctc
     1261 cggcggaggt gggtctggag gcggtggctc ggcaagtttg tacggcttga gaccgaattc
     1321 tcgccctata gtgagtcgta ttacctggcc gtcgttttac aacgtcgtga ctgggaaaac
     1381 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     1441 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     1501 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     1561 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     1621 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     1681 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     1741 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     1801 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     1861 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     1921 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     1981 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     2041 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     2101 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     2161 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     2221 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     2281 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     2341 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     2401 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     2461 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     2521 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     2581 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     2641 gccggtgagc gtggctctcg aggtatcatt gcagcactgg ggccagatgg taagccctcc
     2701 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     2761 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     2821 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     2881 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     2941 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3001 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3061 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     3121 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3181 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3241 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3301 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3361 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3421 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3481 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3541 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3601 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3661 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     3721 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     3781 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     3841 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     3901 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacgagcta tttaggtgac
     3961 actatagaag tgaagctt
//
