LOCUS       pEN-L4-HA-TurboID-linker-R1        4080 bp    DNA     circular UNA 20-MAR-2023
DEFINITION  .
ACCESSION   urn.local...ks-citr5zw
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     attL4           1..97
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL4"
                     /Transferred_Similarity="98.97%"
                     /Primary_Match="98.97%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lr-eztz0t4"">attL4</a>"
     UTR             98..129
                     /modified_by="mamee"
                     /label="omega leader"
     tag             130..213
                     /cds_type="ORF"
                     /modified_by="mamee"
                     /label="3xHA"
     CDS             217..1179
                     /cds_type="ORF"
                     /label="TurboID"
     Linker          1183..1377
                     /cds_type="ORF"
                     /modified_by="mamee"
                     /label="13x Gly4Ser-linker"
     attR1           1378..1502
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR1"
                     /Transferred_Similarity="97.60%"
                     /Primary_Match="97.60%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1c-f0o2y6y"">attR1</a>"
     primer          complement(1557..1577)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="95.24%"
                     /Primary_Match="95.24%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     primer          complement(1577..1598)
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
     rep_origin      complement(1962..2550)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     misc_feature    complement(2728..3537)
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     promoter        complement(3538..3588)
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     primer          4029..4044
                     /invitrogen=1980000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-20) forward primer (pDONRP4-P3 - M13 (-20) forward
                     primer)"
                     /label="M13 (-20) forward primer"
ORIGIN      
        1 taaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
       61 tgctttttta taatgccaac tttgtataga aaagttgaac aacaaacaac aaacaacatt
      121 acaattacaa tgtacccata cgatgttcca gattacgctt acccatacga tgttccagat
      181 tacgcttacc catacgatgt tccagattac gctgccaaag acaataccgt gcccctcaaa
      241 ctaattgcat tgcttgccaa tggtgagttc cattctgggg aacaacttgg ggagactctg
      301 ggcatgtccc gagcggccat taataagcac atacagactt tgagggactg gggtgtcgat
      361 gtgtttacag taccgggtaa gggatattct ctgccggagc cgataccttt actcaatgca
      421 aagcagattt taggacagct ggatggcggg agcgtggctg tcctcccggt ggtcgatagt
      481 acaaatcaat atcttttgga caggatcggt gagctgaaat ctggagatgc ttgcatagcg
      541 gaataccaac aagctggtag gggatctcga ggccgaaagt ggttttcacc ctttggggcc
      601 aacttatact tatccatgtt ctggcgtctg aaacgtggcc cggcagccat agggctaggg
      661 cccgtaattg ggatcgttat ggcggaagcg ttacgaaagt tgggagccga taaagtcaga
      721 gtaaagtggc caaatgatct ttatttacaa gatagaaagc tggcgggcat tcttgttgag
      781 cttgccggaa taacaggcga tgccgcacag atcgttatag gggccgggat taatgtggct
      841 atgaggaggg tcgaagaaag tgttgtgaat caaggatgga ttactctcca agaggcgggt
      901 ataaatcttg atcgaaatac tctagctgca accttgatac gagagctaag agcagcacta
      961 gaattgtttg aacaagaagg gctggcccca tatctccctc gatgggagaa gttggacaat
     1021 tttataaaca gaccggtgaa gttaatcatt ggagacaaag aaattttcgg tataagccgt
     1081 gggatcgata aacagggtgc tcttctcctt gagcaggacg gtgtcatcaa gccttggatg
     1141 ggaggcgaaa tctctctccg aagcgccgag aagaagcttg acggtggcgg aggttctggc
     1201 ggtggaggat ccggaggtgg cgggtccggt ggtggcggat caggtggagg aggttcggga
     1261 ggcggaggta gtggaggcgg tgggagcggc ggaggtggga gcggtggcgg agggagtgga
     1321 ggtggaggct cgggcggagg tggctccggc ggaggtgggt ctggaggcgg tggctcggca
     1381 agtttgtaca aaaaagttga acgagaaacg taaaatgata taaatatcaa tatattaaat
     1441 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat gcagtcacta
     1501 tgatcggatc ccgggcccgt cgactgcaga ggcctgcatg caagcttggt gtaatcatgg
     1561 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     1621 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     1681 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     1741 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     1801 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1861 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1921 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1981 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2041 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2101 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2161 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2221 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2281 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2341 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2401 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2461 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2521 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     2581 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2641 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaagccc aatctgaata
     2701 atgttacaac caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg
     2761 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga
     2821 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat
     2881 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
     2941 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat
     3001 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc
     3061 aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt
     3121 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc
     3181 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg tttttccggg
     3241 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg
     3301 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc
     3361 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaagcg
     3421 atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc
     3481 agcatccatg ttggaattta atcgcggcct cgacgtttcc cgttgaatat ggctcataac
     3541 accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt
     3601 atcttgtgca atgtaacatc agagattttg agacacgggc cagagctgca tcgcgcgttt
     3661 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     3721 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     3781 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     3841 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
     3901 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     3961 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     4021 acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa tacatctaga
//
