LOCUS       pGG-A-2x35S-B           3472 bp    DNA     circular     10-FEB-2023
DEFINITION  .
ACCESSION   p01009
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            VNTDATE|-11477832|                                                 
            VNTDBDATE|-11477832|                                               
            LSOWNER|                                                           
            VNTNAME|pGG-A-RPS5P-B|                                             
            VNTAUTHORNAME|Mansour Karimi|                                      
            VNTAUTHORTEL|003293313838|                                         
            VNTAUTHOREML|manka@psb.vib-ugent.be|                               
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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            0.164644 2.53336                 (274 (CShapeMapEntry 0 "Shape 26"
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            "Label 3") 4                         (CShapeMapEntry 0 "Shape 33" 1
            "Label 21") 29                       (CShapeMapEntry 0 "Shape 16" 1
            "Label 1") 27                        (CShapeMapEntry 2 "Shape 28" 1
            "Label 17") 22                       (CShapeMapEntry 0 "Shape 15" 1
            "Label 0") 21                        (CShapeMapEntry 8 "Shape 17" 1
            "Label 0") 43                        (CShapeMapEntry 8 "Shape 20" 1
            "Label 0") 50                        (CShapeMapEntry 0 "Shape 32" 1
            "Signal Label") 70                   (CShapeMapEntry 0 "Unique
            RSite" 1 "Label 19") 68                   (CShapeMapEntry 0 "RSite"
            1 "Label 19") 67                          (CShapeMapEntry 0 "ORF" 0
            "ORF Label") 94                           (CShapeMapEntry 8 "Shape
            2" 1 "Label 4") 88                         (CShapeMapEntry 9 "Shape
            11" 1 "Label 0") 87                        (CShapeMapEntry 8 "Shape
            18" 1 "Label 0") 86                        (CShapeMapEntry 8 "Shape
            19" 1 "Label 0") 85                        (CShapeMapEntry 8 "Shape
            1" 1 "Signal Label")) 67.6449 67.6449 39 39      0.1 -13986) 1 1 0
            1 1                                              (mapper: 13.2714
            -14.171 67.6449 67.6449 0.01 10 -1 4392 4392 1 0 0)    
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                  (CWidget 22 (7 22 0) 1 2 0 0 Nil -671 100)                   
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FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8a-dgycryy"">A</a>"
                     /label="A"
     promoter        12..797
                     /label="2x35S"
     misc_feature    13..797
                     /Original_Bases="TCGAGGAACTGTAGGAGCTCCCCATAATCGTGAGTAGATAT
                     ATTACTCAACTTTTGATTCGCTATTTGCAGTGCACCTGTGGCGTTCATCACATCTTTT
                     GTGACACTGTTTGCACTGGTCATTGCTATTACAAAGGACCTTCCTGATGTTGAAGGAG
                     ATCGAAAGTAAGTAACTGCACGCATAACCATTTTCTTTCCGCTCTTTGGCTCAATCCA
                     TTTGACAGTCAAAGACAATGTTTAACCAGCTCCGTTTGATATATTGTCTTTATGTGTT
                     TGTTCAAGCATGTTTAGTTAATCATGCCTTTGATTGATCTTGAATAGGTTCCAAATAT
                     CAACCCTGGCAACAAAACTTGGAGTGAGAAACATTGCATTCCTCGGTTCTGGACTTCT
                     GCTAGTAAATTATGTTTCAGCCATATCACTAGCTTTCTACATGCCTCAGGTGAATTCA
                     TCTATTTCCGTCTTAACTATTTCGGTTAATTAAAGCACGAACACCATTACTGCATGTA
                     GAAGCTTGATAAACTATCGCCACCAATTTATTTTTGTTGCGATATTGTTACTTTCCTC
                     AGTATGCAGCTTTGAAAAGACCAACCCTCTTATCCTTTAACAATGAACAGGTTTTTAG
                     AGGTAGCTTGATGATTCCTGCACATGTGATCTTGGCTTCAGGCTTAATTTTCCAGGTA
                     AAGCATTATGAGATACTCTTATATCTCTTACATACTTTTGAGATAATGCACAAGAACT
                     TCATAACTATATGCTTTAGTTTCTGCATTTGACACTGCCAAATTCATTAATCTCTAAT
                     ATCTTTGTTGTTGATCTTTGGTAGACATGGGTACTAGAAAAAGCAAACTACACCAAGG
                     TAAAATACTTTTGTACAAACATAAACTCGTTATCACGGAACATCAATGGAGTGTATAT
                     CTAACGGAGTGTAGAAACATTTGATTATTGCAGGAAGCTATCTCAGGATATTATCGGT
                     TTATATGGAATCTCTTCTACGCAGAGTATCTGTTATTCCCCTTCCTCTAGCTTTCAAT
                     TTCATGGTGAGGATATGCAGTTTTCTTTGTATATCATTCTTCTTCTTCTTTGTAGCTT
                     GGAGTCAAAATCGGTTCCTTCATGTACATACATCAAGGATATGTCCTTCTGAATTTTT
                     ATATCTTGCAATAAAAATGCTTGTACCAATTGAAACACCAGCTTTTTGAGTTCTATGA
                     TCACTGACTTGGTTCTAACCAAAAAAAAAAAAATGTTTAATTTACATATCTAAAAGTA
                     GGTTTAGGGAAACCTAAACAGTAAAATATTTGTATATTATTCGAATTTCACTCATCAT
                     AAAAACTTAAATTGCACCATAAAATTTTGTTTTACTATTAATGATGTAATTTGTGTAA
                     CTTAAGATAAAAATAATATTCCGTAAGTTAACCGGCTAAAACCACGTATAAACCAGGG
                     AACCTGTTAAACCGGTTCTTTACTGGATAAAGAAATGAAAGCCCATGTAGACAGCTCC
                     ATTAGAGCCCAAACCCTAAATTTCTCATCTATATAAAAGGAGTGACATTAGGGTTTTT
                     GTTCGTCCTCTTAAAGCTTCTCGTTTTCTCTGCCGTCTCTCTCATTCGCGCGACGCAA
                     ACGATCTTCAGGTGATCTTCTTTCTCCAAATCCTCTCTCATAACTCTGATTTCGTACT
                     TGTGTATTTGAGCTCACGCTCTGTTTCTCTCACCACAGCC"
                     /label="TCGAGGAACTGTAGGAGCTCCCCATAATCGTGAGTAGATATATTACTCAA
                     CTTTTGATTCGCTATTTGCAGTGCACCTGTGGCGTTCATCACATCTTTTGTGACACTG
                     TTTGCACTGGTCATTGCTATTACAAAGGACCTTCCTGATGTTGAAGGAGATCGAAAGT
                     AAGTAACTGCACGCATAACCATTTTCTTTCCGCTCTTTGGCTCAATCCATTTGACAGT
                     CAAAGACAATGTTTAACCAGCTCCGTTTGATATATTGTCTTTATGTGTTTGTTCAAGC
                     ATGTTTAGTTAATCATGCCTTTGATTGATCTTGAATAGGTTCCAAATATCAACCCTGG
                     CAACAAAACTTGGAGTGAGAAACATTGCATTCCTCGGTTCTGGACTTCTGCTAGTAAA
                     TTATGTTTCAGCCATATCACTAGCTTTCTACATGCCTCAGGTGAATTCATCTATTTCC
                     GTCTTAACTATTTCGGTTAATTAAAGCACGAACACCATTACTGCATGTAGAAGCTTGA
                     TAAACTATCGCCACCAATTTATTTTTGTTGCGATATTGTTACTTTCCTCAGTATGCAG
                     CTTTGAAAAGACCAACCCTCTTATCCTTTAACAATGAACAGGTTTTTAGAGGTAGCTT
                     GATGATTCCTGCACATGTGATCTTGGCTTCAGGCTTAATTTTCCAGGTAAAGCATTAT
                     GAGATACTCTTATATCTCTTACATACTTTTGAGATAATGCACAAGAACTTCATAACTA
                     TATGCTTTAGTTTCTGCATTTGACACTGCCAAATTCATTAATCTCTAATATCTTTGTT
                     GTTGATCTTTGGTAGACATGGGTACTAGAAAAAGCAAACTACACCAAGGTAAAATACT
                     TTTGTACAAACATAAACTCGTTATCACGGAACATCAATGGAGTGTATATCTAACGGAG
                     TGTAGAAACATTTGATTATTGCAGGAAGCTATCTCAGGATATTATCGGTTTATATGGA
                     ATCTCTTCTACGCAGAGTATCTGTTATTCCCCTTCCTCTAGCTTTCAATTTCATGGTG
                     AGGATATGCAGTTTTCTTTGTATATCATTCTTCTTCTTCTTTGTAGCTTGGAGTCAAA
                     ATCGGTTCCTTCATGTACATACATCAAGGATATGTCCTTCTGAATTTTTATATCTTGC
                     AATAAAAATGCTTGTACCAATTGAAACACCAGCTTTTTGAGTTCTATGATCACTGACT
                     TGGTTCTAACCAAAAAAAAAAAAATGTTTAATTTACATATCTAAAAGTAGGTTTAGGG
                     AAACCTAAACAGTAAAATATTTGTATATTATTCGAATTTCACTCATCATAAAAACTTA
                     AATTGCACCATAAAATTTTGTTTTACTATTAATGATGTAATTTGTGTAACTTAAGATA
                     AAAATAATATTCCGTAAGTTAACCGGCTAAAACCACGTATAAACCAGGGAACCTGTTA
                     AACCGGTTCTTTACTGGATAAAGAAATGAAAGCCCATGTAGACAGCTCCATTAGAGCC
                     CAAACCCTAAATTTCTCATCTATATAAAAGGAGTGACATTAGGGTTTTTGTTCGTCCT
                     CTTAAAGCTTCTCGTTTTCTCTGCCGTCTCTCTCATTCGCGCGACGCAAACGATCTTC
                     AGGTGATCTTCTTTCTCCAAATCCTCTCTCATAACTCTGATTTCGTACTTGTGTATTT
                     GAGCTCACGCTCTGTTTCTCTCACCACAGCC"
                     /note="Geneious type: Editing History Replacement"
     GG              complement(798..808)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7z-dgycryx"">B</a>"
                     /label="B"
     primer          complement(828..848)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1328..1432
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1433..2293
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2464..3052
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3416..3437
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc tcccaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag
       61 atacagtctc agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa
      121 acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg
      181 aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct
      241 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
      301 acgttccaac cacgtcttca aagcaagtgg attgatgtga acatggtgga gcacgacact
      361 ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact
      421 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac
      481 ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa
      541 ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc
      601 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
      661 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
      721 tcctctatat aaggaagttc atttcatttg gagaggacac gctgaaatca ccagtctctc
      781 tctacaaatc tatctctaac atgagaccga attctcgccc tatagtgagt cgtattacct
      841 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      901 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      961 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
     1021 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     1081 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     1141 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     1201 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     1261 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     1321 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1381 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     1441 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     1501 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     1561 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     1621 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     1681 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     1741 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     1801 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     1861 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     1921 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     1981 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     2041 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2101 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggct ctcgaggtat
     2161 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2221 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2281 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2341 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2401 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2461 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2521 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     2581 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     2641 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     2701 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2761 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2821 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2881 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2941 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3001 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3061 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3121 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3181 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     3241 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     3301 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     3361 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     3421 cggataacaa tttcacacga gctatttagg tgacactata gaagtgaagc tt
//
