LOCUS       pGG-C-Cas9(H840A)-D        6792 bp    DNA     circular UNA 10-FEB-2023
DEFINITION  .
ACCESSION   p01945
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:88-dgycryy"">C</a>"
                     /label="C"
     CDS             14..4117
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AtCas9-H840A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:3w-dpz6pwd"">AtCas9-H840A</a>"
                     /Alternative_Match="99.98%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:44-dpz6qit"">AtCas9-D10A,H840A</a>"
                     /Alternative_Match="99.95%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:95-dgycryy"">AtCas9</a>"
                     /Alternative_Match="99.93%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:3r-dpz6pul"">AtCas9-D10A</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:3/0; <a
                     href=""urn:local:.:9s-dgycryz"">SpCas9(H840A)</a>"
                     /Alternative_Match="99.93%; Adjusted-Interval-0:3/0; <a
                     href=""urn:local:.:9u-dgycryz"">SpCas9</a>"
                     /Alternative_Match="99.93%; Adjusted-Interval-0:3/0; <a
                     href=""urn:local:.:9w-dgycryz"">SpCas9(D10A,H840A)</a>"
                     /Alternative_Match="99.85%; Adjusted-Interval-0:3/0; <a
                     href=""urn:local:.:9t-dgycryz"">SpCas9(D10A)</a>"
                     /label="AtCAS9-H840A"
     modified_base   2531..2533
                     /created_by="mamee"
                     /label="H840A"
     GG              complement(4118..4128)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:81-dgycryx"">D</a>"
                     /label="D"
     primer          complement(4148..4168)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        4648..4752
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    4753..5613
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      5784..6372
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          6736..6757
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tcgatggata agaagtactc tatcggactc gatatcggaa ctaactctgt
       61 gggatgggct gtgatcaccg atgagtacaa ggtgccatct aagaagttca aggttctcgg
      121 aaacaccgat aggcactcta tcaagaaaaa ccttatcggt gctctcctct tcgattctgg
      181 tgaaactgct gaggctacca gactcaagag aaccgctaga agaaggtaca ccagaagaaa
      241 gaacaggatc tgctacctcc aagagatctt ctctaacgag atggctaaag tggatgattc
      301 attcttccac aggctcgaag agtcattcct cgtggaagaa gataagaagc acgagaggca
      361 ccctatcttc ggaaacatcg ttgatgaggt ggcataccac gagaagtacc ctactatcta
      421 ccacctcaga aagaagctcg ttgattctac tgataaggct gatctcaggc tcatctacct
      481 cgctctcgct cacatgatca agttcagagg acacttcctc atcgagggtg atctcaaccc
      541 tgataactct gatgtggata agttgttcat ccagctcgtg cagacctaca accagctttt
      601 cgaagagaac cctatcaacg cttcaggtgt ggatgctaag gctatcctct ctgctaggct
      661 ctctaagtca agaaggcttg agaacctcat tgctcagctc cctggtgaga agaagaacgg
      721 acttttcgga aacttgatcg ctctctctct cggactcacc cctaacttca agtctaactt
      781 cgatctcgct gaggatgcaa agctccagct ctcaaaggat acctacgatg atgatctcga
      841 taacctcctc gctcagatcg gagatcagta cgctgatttg ttcctcgctg ctaagaacct
      901 ctctgatgct atcctcctca gtgatatcct cagagtgaac accgagatca ccaaggctcc
      961 actctcagct tctatgatca agagatacga tgagcaccac caggatctca cacttctcaa
     1021 ggctcttgtt agacagcagc tcccagagaa gtacaaagag attttcttcg atcagtctaa
     1081 gaacggatac gctggttaca tcgatggtgg tgcatctcaa gaagagttct acaagttcat
     1141 caagcctatc ctcgagaaga tggatggaac cgaggaactc ctcgtgaagc tcaatagaga
     1201 ggatcttctc agaaagcaga ggaccttcga taacggatct atccctcatc agatccacct
     1261 cggagagttg cacgctatcc ttagaaggca agaggatttc tacccattcc tcaaggataa
     1321 cagggaaaag attgagaaga ttctcacctt cagaatccct tactacgtgg gacctctcgc
     1381 tagaggaaac tcaagattcg cttggatgac cagaaagtct gaggaaacca tcaccccttg
     1441 gaacttcgaa gaggtggtgg ataagggtgc tagtgctcag tctttcatcg agaggatgac
     1501 caacttcgat aagaaccttc caaacgagaa ggtgctccct aagcactctt tgctctacga
     1561 gtacttcacc gtgtacaacg agttgaccaa ggttaagtac gtgaccgagg gaatgaggaa
     1621 gcctgctttt ttgtcaggtg agcaaaagaa ggctatcgtt gatctcttgt tcaagaccaa
     1681 cagaaaggtg accgtgaagc agctcaaaga ggattacttc aagaaaatcg agtgcttcga
     1741 ttcagttgag atttctggtg ttgaggatag gttcaacgca tctctcggaa cctaccacga
     1801 tctcctcaag atcattaagg ataaggattt cttggataac gaggaaaacg aggatatctt
     1861 ggaggatatc gttcttaccc tcaccctctt tgaagataga gagatgattg aagaaaggct
     1921 caagacctac gctcatctct tcgatgataa ggtgatgaag cagttgaaga gaagaagata
     1981 cactggttgg ggaaggctct caagaaagct cattaacgga atcagggata agcagtctgg
     2041 aaagacaatc cttgatttcc tcaagtctga tggattcgct aacagaaact tcatgcagct
     2101 catccacgat gattctctca cctttaaaga ggatatccag aaggctcagg tttcaggaca
     2161 gggtgatagt ctccatgagc atatcgctaa cctcgctgga tctcctgcaa tcaagaaggg
     2221 aatcctccag actgtgaagg ttgtggatga gttggtgaag gtgatgggaa ggcataagcc
     2281 tgagaacatc gtgatcgaaa tggctagaga gaaccagacc actcagaagg gacagaagaa
     2341 ctctagggaa aggatgaaga ggatcgagga aggtatcaaa gagcttggat ctcagatcct
     2401 caaagagcac cctgttgaga acactcagct ccagaatgag aagctctacc tctactacct
     2461 ccagaacgga agggatatgt atgtggatca agagttggat atcaacaggc tctctgatta
     2521 cgatgttgat gctatcgtgc cacagtcatt cttgaaggat gattctatcg ataacaaggt
     2581 gctcaccagg tctgataaga acaggggtaa gagtgataac gtgccaagtg aagaggttgt
     2641 gaagaaaatg aagaactatt ggaggcagct cctcaacgct aagctcatca ctcagagaaa
     2701 gttcgataac ttgactaagg ctgagagggg aggactctct gaattggata aggcaggatt
     2761 catcaagagg cagcttgtgg aaaccaggca gatcactaag cacgttgcac agatcctcga
     2821 ttctaggatg aacaccaagt acgatgagaa cgataagttg atcagggaag tgaaggttat
     2881 caccctcaag tcaaagctcg tgtctgattt cagaaaggat ttccaattct acaaggtgag
     2941 ggaaatcaac aactaccacc acgctcacga tgcttacctt aacgctgttg ttggaaccgc
     3001 tctcatcaag aagtatccta agctcgagtc agagttcgtg tacggtgatt acaaggtgta
     3061 cgatgtgagg aagatgatcg ctaagtctga gcaagagatc ggaaaggcta ccgctaagta
     3121 tttcttctac tctaacatca tgaatttctt caagaccgag attaccctcg ctaacggtga
     3181 gatcagaaag aggccactca tcgagacaaa cggtgaaaca ggtgagatcg tgtgggataa
     3241 gggaagggat ttcgctaccg ttagaaaggt gctctctatg ccacaggtga acatcgttaa
     3301 gaaaaccgag gtgcagaccg gtggattctc taaagagtct atcctcccta agaggaactc
     3361 tgataagctc attgctagga agaaggattg ggaccctaag aaatacggtg gtttcgattc
     3421 tcctaccgtg gcttactctg ttctcgttgt ggctaaggtt gagaagggaa agagtaagaa
     3481 gctcaagtct gttaaggaac ttctcggaat cactatcatg gaaaggtcat ctttcgagaa
     3541 gaacccaatc gatttcctcg aggctaaggg atacaaagag gttaagaagg atctcatcat
     3601 caagctccca aagtactcac tcttcgaact cgagaacggt agaaagagga tgctcgcttc
     3661 tgctggtgag cttcaaaagg gaaacgagct tgctctccca tctaagtacg ttaactttct
     3721 ttacctcgct tctcactacg agaagttgaa gggatctcca gaagataacg agcagaagca
     3781 acttttcgtt gagcagcaca agcactactt ggatgagatc atcgagcaga tctctgagtt
     3841 ctctaaaagg gtgatcctcg ctgatgcaaa cctcgataag gtgttgtctg cttacaacaa
     3901 gcacagagat aagcctatca gggaacaggc agagaacatc atccatctct tcacccttac
     3961 caacctcggt gctcctgctg ctttcaagta cttcgataca accatcgata ggaagagata
     4021 cacctctacc aaagaagtgc tcgatgctac cctcatccat cagtctatca ctggactcta
     4081 cgagactagg atcgatctct cacagctcgg tggtgattca gtgagaccga attctcgccc
     4141 tatagtgagt cgtattacct ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     4201 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     4261 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     4321 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     4381 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     4441 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     4501 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     4561 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     4621 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4681 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4741 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4801 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4861 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4921 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4981 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5041 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5101 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5161 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5221 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5281 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5341 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5401 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5461 gagcgtggct ctcgaggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5521 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5581 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5641 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5701 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5761 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5821 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5881 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5941 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6001 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6061 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6121 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6181 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6241 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6301 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6361 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6421 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6481 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     6541 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     6601 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     6661 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     6721 atgttgtgtg gaattgtgag cggataacaa tttcacacga gctatttagg tgacactata
     6781 gaagtgaagc tt
//
