LOCUS       pGG-A-pZmUBIL-B,2        4673 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local.laurenspauwels.19-cb8i5i3
VERSION     urn.local.laurenspauwels.19-cb8i5i3
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8a-dgycryy"">A</a>"
                     /label="A"
     promoter        12..1998
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBIL.2
                     (<a href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /modified_by="mamee"
                     /label="ZmUBIL.2"
     intron          989..1998
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBI
                     intron (<a href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBI intron"
     GG              complement(1999..2009)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7z-dgycryx"">B</a>"
                     /label="B"
     primer          complement(2029..2049)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        2529..2633
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    2634..3494
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3665..4253
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4617..4638
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc tgtgcagcgt gacccggtcg tgcccctctc tagagataat gagcattgca
       61 tgtctaagtt ataaaaaatt accacatatt ttttttgtca cacttgtttg aagtgcagtt
      121 tatctatctt tatacatata tttaaacttt actctacgaa taatataatc tatagtacta
      181 caataatatc agtgttttag agaatcatat aaatgaacag ttagacatgg tctaaaggac
      241 aattgagtat tttgacaaca ggactctaca gttttatctt tttagtgtgc atgtgttctc
      301 cttttttttt gcaaatagct tcacctatat aatacttcat ccattttatt agtacatcca
      361 tttagggttt agggttaatg gtttttatag actaattttt ttagtacatc tattttattc
      421 tattttagcc tctaaattaa gaaaactaaa actctatttt agttttttta tttaataatt
      481 tagatataaa atagaataaa ataaagtgac taaaaattaa acaaataccc tttaagaaat
      541 taaaaaaact aaggaaacat ttttcttgtt tcgagtagat aatgccagcc tgttaaacgc
      601 cgtcgacgag tctaacggac accaaccagc gaaccagcag cgtcgcgtcg ggccaagcga
      661 agcagacggc acggcatctc tgtcgctgcc tctggacccc tctcgagagt tccgctccac
      721 cgttggactt gctccgctgt cggcatccag aaattgcgtg gcggagcggc agacgtgagc
      781 cggcacggca ggcggcctcc tcctcctctc acggcacggc agctacgggg gattcctttc
      841 ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata aatagacacc ccctccacac
      901 cctctttccc caacctcgtg ttgttcggag cgcacacaca cacaaccaga tctcccccaa
      961 atccacccgt cggcacctcc gcttcaaggt acgccgctcg tcctcccccc ccccccctct
     1021 ctaccttctc tagatcggcg ttccggtcca tggttagggc ccggtagttc tacttctgtt
     1081 catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat
     1141 gcgacctgta cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat
     1201 cctgggatgg ctctagccgt tccgcagacg ggatcgattt catgattttt tttgtttcgt
     1261 tgcatagggt ttggtttgcc cttttccttt atttcaatat atgccgtgca cttgtttgtc
     1321 gggtcatctt ttcatgcttt tttttgtctt ggttgtgatg atgtggtctg gttgggcggt
     1381 cgttctagat cggagtagaa ttctgtttca aactacctgg tggatttatt aattttggat
     1441 ctgtatgtgt gtgccataca tattcatagt tacgaattga agatgatgga tggaaatatc
     1501 gatctaggat aggtatacat gttgatgcgg gttttactga tgcatataca gagatgcttt
     1561 ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg ttctagatcg
     1621 gagtagaata ctgtttcaaa ctacctggtg tatttattaa ttttggaact gtatgtgtgt
     1681 gtcatacatc ttcatagtta cgagtttaag atggatggaa atatcgatct aggataggta
     1741 tacatgttga tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca
     1801 tatgctctaa ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt
     1861 gatcttgata tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct
     1921 gccttcatac gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt
     1981 ttggtgttac ttctgcagaa catgagaccg aattctcgcc ctatagtgag tcgtattacc
     2041 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     2101 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     2161 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
     2221 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     2281 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     2341 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     2401 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     2461 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     2521 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     2581 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     2641 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     2701 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     2761 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     2821 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     2881 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2941 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     3001 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     3061 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3121 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     3181 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     3241 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     3301 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggc tctcgaggta
     3361 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     3421 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     3481 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     3541 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     3601 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3661 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3721 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3781 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3841 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3901 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3961 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4021 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4081 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4141 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4201 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4261 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4321 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4381 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4441 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     4501 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     4561 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     4621 gcggataaca atttcacacg agctatttag gtgacactat agaagtgaag ctt
//
