LOCUS       pH-U1-A-ccdB-G-U9       10104 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...3ct-ak3co46
VERSION     urn.local...3ct-ak3co46
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##PGE_Collection_info-START##                                      
            Plasmid ID             :: p01720                                   
            E. coli PGE ID         :: PGE02520                                 
            Agro PGE ID            :: NA                                       
            Name                   :: pH-U9-G-ccdB-A-U1                        
            Vector Type            ::                                          
            E. coli selection      :: Spectinomycin                            
            Plant Selection        :: Hygromycin                               
            Description            ::                                          
            Golden Gate Collection ::                                          
            References             :: from Mansour                             
            Entered by             :: manka                                    
            Cloning Sites          :: NA                                       
            Backbone               :: pH                                       
            ##PGE_Collection_info-END##                                        
            ##Plasmid_Information_(wacae)-START##                              
            Plasmid ID          :: p01720                                      
            Agro PGE ID         :: NA                                          
            Addgene ID          :: NA                                          
            Reference           :: unpublished                                 
            Bacterial Selection :: Spectinomycin                               
            Cloning Sites       :: U1-U9                                       
            ##Plasmid_Information_(wacae)-END##
FEATURES             Location/Qualifiers
     Promoter        3..277
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    278..1069
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1315..1903
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     regulatory      complement(1939..2199)
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /Alternative_Match="99.29%; Adjusted-Interval-0:30/150; <a
                     href=""urn:local:.:79-dlucaql"">bom</a>"
                     /label="pBR322 bom"
     rep_origin      2573..2767
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     regulatory      complement(2833..3906)
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     regulatory      complement(4335..4964)
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     T-DNA_border    6150..6380
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6f-dgycryw"">RB</a>"
                     /Alternative_Match="99.50%; Adjusted-Interval-0:30/0; <a
                     href=""urn:local:.:3c-djznfva"">RB</a>"
                     /label="RB"
     misc_feature    6388..6427
                     /created_by="lapau"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7p-dgycryx"">U1</a>"
                     /label="U1"
                     /note="Geneious type: Golden Gibson Cloning site"
     misc_difference complement(6428..6445)
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7y-dgycryx"">I-SceI site</a>"
                     /label="I-SceI site"
     GG              complement(6446..6456)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:85-dgycryy"">A</a>"
                     /label="A"
     CDS             complement(6463..6768)
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.67%"
                     /label="ccdB"
     UTR             complement(6769..7087)
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.37%"
                     /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    complement(7110..7769)
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="99.85%"
                     /Primary_Match="99.85%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     promoter        complement(7823..7853)
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     GG              7870..7880
                     /%_Identity=100
                     /Motif="GGTCTCAGTAT"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="G"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:86-dgycryy"">G</a>"
                     /label="G"
     misc_difference 7881..7898
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7y-dgycryx"">I-SceI site</a>"
                     /label="I-SceI site"
     misc_feature    7899..7938
                     /standard_name="U9"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U9"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7x-dgycryx"">U9</a>"
                     /note="Geneious type: Golden Gibson Cloning site"
     Plant_Selection 7972..9751
                     /created_by="mamee"
                     /label="Hygro"
     promoter        7972..8259
                     /created_by="thjac"
                     /modified_by="wacae"
                     /Transferred_From="pNOS"
                     /Transferred_Similarity="100.00%"
                     /label="pNOS"
     CDS             8274..9299
                     /modified_by="wacae"
                     /Transferred_From="Hyg"
                     /Transferred_Similarity="100.00%"
                     /label="Hyg"
     terminator      9484..9751
                     /Transferred_From="NOST"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="tNOS"
     T-DNA_border    9772..10104
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
ORIGIN      
        1 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
       61 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
      121 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
      181 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
      241 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga
      301 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
      361 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
      421 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
      481 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
      541 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
      601 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
      661 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
      721 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
      781 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
      841 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
      901 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
      961 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     1021 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag
     1081 aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta
     1141 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg
     1201 tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg
     1261 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1321 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1381 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1441 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     1501 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1561 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1621 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1681 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     1741 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     1801 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     1861 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     1921 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     1981 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2041 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     2101 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     2161 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     2221 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     2281 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     2341 tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag
     2401 tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt
     2461 tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga
     2521 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca
     2581 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc
     2641 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg
     2701 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct
     2761 atccacagga aagagagctt ttcgaccttt ttcccctgct agggcaattt gccctagcat
     2821 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat
     2881 gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt
     2941 tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact
     3001 gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc
     3061 caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt
     3121 cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat
     3181 ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc
     3241 ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc
     3301 aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc
     3361 atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg
     3421 cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat
     3481 cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac
     3541 gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag
     3601 acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat
     3661 cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc
     3721 ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc
     3781 accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt
     3841 ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc
     3901 gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc
     3961 cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg
     4021 gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc
     4081 atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt
     4141 aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa
     4201 ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt
     4261 gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc
     4321 tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg
     4381 cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct
     4441 gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc
     4501 gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc
     4561 gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg
     4621 gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg
     4681 atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc
     4741 cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc
     4801 ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact
     4861 aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc
     4921 ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta
     4981 tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact
     5041 caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg
     5101 gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg
     5161 agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt
     5221 cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg
     5281 gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc
     5341 accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag
     5401 ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc
     5461 gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt
     5521 cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg
     5581 ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt
     5641 gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc
     5701 aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac
     5761 gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc
     5821 ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt
     5881 ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct
     5941 cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa
     6001 acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga
     6061 cttgtgcgac atgtcgtttt cagaagaggg ctgcactgaa cgtcagaagc cgactgcact
     6121 atagcagcgg aggggttgga tcaaagtact ttaaagtact ttaaagtact ttaaagtact
     6181 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
     6241 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
     6301 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
     6361 atccaagctc aagctaagct tgagctccat tactcgcatc cattctcagg ctgtctcgtc
     6421 tcgtctctag ggataacagg gtaatacctt gagaccgtcg acttatattc cccagaacat
     6481 caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca cttcttcccc
     6541 gataacggag actggcacac tggccatatc ggtggtcatc atgcgccagc tttcatcccc
     6601 gatatgcacc accgggtaaa gttcacggga gactttatct gacagcagac gtgcactggc
     6661 cagggggatc accatccgtc gcccgggcgt gtcaataata tcactctgta catccacaaa
     6721 cagacgataa cggctctctc ttttataggt gtaaacctta aactgcattt caccagtccc
     6781 tgttctcgtc agcaaaagag ccgttcattt caataaaccg ggcgacctca gccatccctt
     6841 cctgattttc cgctttccag cgttcggcac gcagacgacg ggcttcattc tgcatggttg
     6901 tgcttaccag accggagata ttgacatcat atatgccttg agcaactgat agctgtcgct
     6961 gtcaactgtc actgtaatac gctgcttcat agcacacctc tttttgacat acttcgggta
     7021 tacatatcag tatatattct tataccgcaa aaatcagcgc gcaaatacgc atactgttat
     7081 ctggctttta gtaagccgga tccacgcgtt tacgccccgc cctgccactc atcgcagtac
     7141 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacagacggc atgatgaacc
     7201 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     7261 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     7321 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     7381 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     7441 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     7501 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga
     7561 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     7621 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     7681 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     7741 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgcc
     7801 ggatcctaac tcaaaatcca cacattatac gagccggaag cataaagtgt aaagcctggg
     7861 gtgcctaatg gtctcagtat attaccctgt tatccctagt tccttatcat ctggcgaatc
     7921 ggacccacaa gagcactggg gccctctaga ggatccccgg gtaccgcgaa ttatcgatca
     7981 tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg acaagccgtt
     8041 ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actgagccgc gggtttctgg
     8101 agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa agtcgcctaa
     8161 ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt tccataaatt
     8221 cccctcggta tccaattaga gtctcatatt cactctcaac tcgaatcccc cctatgaaaa
     8281 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     8341 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     8401 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     8461 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggagt
     8521 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     8581 tgcctgaaac cgaactgccc gctgttcttc agccggtcgc ggaggctatg gatgctatcg
     8641 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     8701 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     8761 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     8821 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     8881 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     8941 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     9001 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccaaggctcc
     9061 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     9121 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     9181 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     9241 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataga
     9301 gtagatgccg accgaacaag agctgatttc gagaacgcct cagccagcaa ctcgcgcgag
     9361 cctagcaagg caaatgcgag agaacggcct tacgcttggt ggcacagttc tcgtccacag
     9421 ttcgctaagc tcgctcggct gggtcgcggg agggccggtc gcagtgattc aggaattaat
     9481 tccctagagt caagcagatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
     9541 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
     9601 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
     9661 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
     9721 gcgcgcggtg tcatctatgt tactagatcg accggcatgc aagctgataa ttcaattcgg
     9781 cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt
     9841 tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg
     9901 gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag
     9961 ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta
    10021 agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg
    10081 acagagcgtt gctgcctgtg atca
//
