LOCUS       pHb-U1-A-ccdB-G-U9       10696 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...2r-e3ydi39
VERSION     urn.local...2r-e3ydi39
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     Promoter        8..289
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="96.81%"
                     /Primary_Match="96.81%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    290..1081
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1327..1915
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     regulatory      complement(1951..2211)
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /label="pBR322 bom"
     rep_origin      2585..2779
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     Domestication   2789
                     /standard_name="G->C"
     regulatory      complement(2845..3918)
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     regulatory      complement(4347..4976)
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     Domestication   6100
                     /standard_name="G->C"
     T-DNA_border    6162..6392
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6f-dgycryw"">RB</a>"
                     /label="RB"
     misc_feature    6400..6439
                     /created_by="lapau"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7p-dgycryx"">U1</a>"
                     /label="U1"
                     /note="Geneious type: Golden Gibson Cloning site"
     misc_difference complement(6440..6457)
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7y-dgycryx"">I-SceI site</a>"
                     /label="I-SceI site"
     GG              complement(6458..6468)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:85-dgycryy"">A</a>"
                     /label="A"
     CDS             complement(6475..6780)
                     /standard_name="ccdB"
     UTR             complement(6781..7099)
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.37%"
                     /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    complement(7122..7781)
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="99.85%"
                     /Primary_Match="99.85%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     promoter        complement(7835..7865)
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     GG              7882..7892
                     /%_Identity=100
                     /Motif="GGTCTCAGTAT"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="G"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:86-dgycryy"">G</a>"
                     /label="G"
     misc_difference 7893..7910
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7y-dgycryx"">I-SceI site</a>"
                     /label="I-SceI site"
     misc_feature    7911..7950
                     /standard_name="U9"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U9"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7x-dgycryx"">U9</a>"
                     /note="Geneious type: Golden Gibson Cloning site"
     Plant_Selection 7984..10329
                     /created_by="mamee"
                     /label="Hygro"
     promoter        7984..8847
                     /regulatory_class="promoter"
                     /gene="CaMV 35S"
                     /note="internal BsaI recognition site removed"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCaMV 35S"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (<a
                     href=""urn:local:.:6v-dgycryx"">pCaMV 35S</a>)"
                     /label="p35S"
     CDS             8855..9877
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Hyg"
                     /Transferred_Similarity="99.71%"
                     /Primary_Match="99.71%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:90-dgycryy"">Hyg</a>"
                     /label="Hyg"
     terminator      10062..10329
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="NOST"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (<a
                     href=""urn:local:.:6c-dgycryw"">NOST</a>)"
                     /label="tNOS"
     T-DNA_border    10364..10696
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
ORIGIN      
        1 attcgggcac gaacccagtg gacataagcc tcgttcggtt cgtaagctgt aatgcaagta
       61 gcgtaactgc cgtcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
      121 gcagtggcgg ttttcatggc ttcttgttat gacatgtttt tttggggtac agtctatgcc
      181 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
      241 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc
      301 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
      361 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
      421 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
      481 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
      541 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
      601 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
      661 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
      721 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
      781 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
      841 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
      901 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
      961 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     1021 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     1081 atgtctagct agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg
     1141 ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat
     1201 tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat gcatgaccaa
     1261 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1321 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1381 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1441 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     1501 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1561 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1621 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1681 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1741 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1801 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1861 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1921 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1981 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     2041 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     2101 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     2161 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     2221 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     2281 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     2341 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg
     2401 ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag
     2461 gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag
     2521 ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga
     2581 cagttatgca caggccaggc gggttttaag agttttaata agttttaaag agttttaggc
     2641 ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat
     2701 gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc
     2761 aatgtacgtg ctatccacag gaaagagagc ttttcgacct ttttcccctg ctagggcaat
     2821 ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt
     2881 gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc
     2941 cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc
     3001 ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc
     3061 ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc
     3121 ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc
     3181 agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg
     3241 gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt
     3301 acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt
     3361 ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa
     3421 cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg
     3481 ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc
     3541 ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc
     3601 acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac
     3661 gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat
     3721 cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc
     3781 ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc
     3841 ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga
     3901 tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc
     3961 cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat
     4021 tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa
     4081 attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga
     4141 tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc
     4201 ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa
     4261 cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt
     4321 gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc
     4381 gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg
     4441 gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg
     4501 tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac
     4561 acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc
     4621 accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag
     4681 tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact
     4741 acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg
     4801 acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga
     4861 tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt
     4921 gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt
     4981 tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca
     5041 agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg
     5101 ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca
     5161 gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg
     5221 cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc
     5281 ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc
     5341 ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag
     5401 tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg
     5461 gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg
     5521 ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat
     5581 gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc
     5641 cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg
     5701 gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt
     5761 cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc
     5821 gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc
     5881 ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc
     5941 agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt
     6001 tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg
     6061 cgtaacttag gacttgtgcg acatgtcgtt ttcagaagag ggctgcactg aacgtcagaa
     6121 gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttaaagta ctttaaagta
     6181 ctttaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg
     6241 aacggataaa ccttttcacg cccttttaaa tatccgatta ttctaataaa cgctcttttc
     6301 tcttaggttt acccgccaat atatcctgtc aaacactgat agtttaaact gaaggcggga
     6361 aacgacaatc tgatccaagc tcaagctaag cttgagctcc attactcgca tccattctca
     6421 ggctgtctcg tctcgtctct agggataaca gggtaatacc ttgagaccgt cgacttatat
     6481 tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc
     6541 cacttcttcc ccgataacgg agactggcac actggccata tcggtggtca tcatgcgcca
     6601 gctttcatcc ccgatatgca ccaccgggta aagttcacgg gagactttat ctgacagcag
     6661 acgtgcactg gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg
     6721 tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat
     6781 ttcaccagtc cctgttctcg tcagcaaaag agccgttcat ttcaataaac cgggcgacct
     6841 cagccatccc ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat
     6901 tctgcatggt tgtgcttacc agaccggaga tattgacatc atatatgcct tgagcaactg
     6961 atagctgtcg ctgtcaactg tcactgtaat acgctgcttc atagcacacc tctttttgac
     7021 atacttcggg tatacatatc agtatatatt cttataccgc aaaaatcagc gcgcaaatac
     7081 gcatactgtt atctggcttt tagtaagccg gatccacgcg tttacgcccc gccctgccac
     7141 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacagacg
     7201 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     7261 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     7321 gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg
     7381 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     7441 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
     7501 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     7561 cggaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     7621 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     7681 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     7741 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     7801 gaaaatctcg ccggatccta actcaaaatc cacacattat acgagccgga agcataaagt
     7861 gtaaagcctg gggtgcctaa tggtctcagt atattaccct gttatcccta gttccttatc
     7921 atctggcgaa tcggacccac aagagcactg gggccctcta gaggatcccc gggtaccgcg
     7981 aatccgatcc attcggtccc cagattagcc ttttcaattt cagaaagaat gctaacccac
     8041 agatggttag agaggcttac gcagcacgtc tcatcaagac gatctacccg agcaataatc
     8101 tccaggaaat caaatacctt cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta
     8161 actgcatcaa gaacacagag aaagatatat ttctcaagat cagaagtact attccagtat
     8221 ggacgattca aggcttgctt cacaaaccaa ggcaagtaat agagattgga gtctctaaaa
     8281 aggtagttcc cactgaatca aaggccatgg agtcaaagat tcaaatagag gacctaacag
     8341 aactcgccgt aaagactggc gaacagttca tacagagtct cttacgactc aatgacaaga
     8401 agaaaatctt cgtcaacatg gtggagcacg acacacttgt ctactccaaa aatatcaaag
     8461 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa
     8521 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg
     8581 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct
     8641 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
     8701 acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg
     8761 atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc
     8821 atttggagag aacacggggg acgagctcct gcagatgaaa aagcctgaac tcaccgcgac
     8881 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     8941 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     9001 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     9061 ggccgcgctc ccgattccgg aagtgcttga cattggggag ttcagcgaga gcctgaccta
     9121 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     9181 cgctgttctt cagccggtcg cggaggctat ggatgctatc gctgcggccg atcttagcca
     9241 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     9301 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     9361 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggcga ggactgcccc
     9421 gaagtccggc actcgtgcac gcggattcgg ctccaacaat gtcctgacgg acaatggccg
     9481 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     9541 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     9601 gcggaggcat ccggagcttg caggatcgcc aaggctccgg gcgtatatgc tccgcattgg
     9661 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     9721 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     9781 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     9841 ccgacgcccc agcactcgtc cgagggcaaa ggaatagagt agatgccgac cgaacaagag
     9901 ctgatttcga gaacgcctca gccagcaact cgcgcgagcc tagcaaggca aatgcgagag
     9961 aacggcctta cgcttggtgg cacagttctc gtccacagtt cgctaagctc gctcggctgg
    10021 gtcgcgggag ggccggtcgc agtgattcag gaattaattc cctagagtca agcagatcgt
    10081 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
    10141 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
    10201 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
    10261 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
    10321 ctagatcgac cggcatgcaa gctggggtac cctcgaattc aattcaattc ggcgttaatt
    10381 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
    10441 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
    10501 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
    10561 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
    10621 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
    10681 ttgctgcctg tgatca
//
