LOCUS       pGG-F-AarI-SacB-AarI-G        5074 bp    DNA     circular UNA 06-FEB-2023
DEFINITION  .
ACCESSION   urn.local...8r-a1sdsfb
VERSION     urn.local...8r-a1sdsfb
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8c-dgycryy"">F</a>"
                     /label="F"
     promoter        23..468
                     /gene="sacR"
                     /note="sacB promoter and control region"
                     /modified_by="mamee"
                     /label="pSacB"
     CDS             469..1890
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /note="negative selection marker"
                     /Original_Translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQK
                     PYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNA
                     DGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDA
                     NDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNIN
                     GVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGT
                     EDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLK
                     KVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNS
                     LTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQS
                     TFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK"
                     /label="SacB"
     GG              complement(1902..1912)
                     /created_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="G"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:84-dgycryy"">G</a>"
                     /label="G"
     misc_feature    complement(2687..3220)
                     /modified_by="wacae"
                     /Transferred_From="GmR"
                     /Transferred_Similarity="100.00%"
                     /label="GmR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      4094..4682
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
ORIGIN      
        1 ggtctcaact aggatgcagg tgcacatata cctgccgttc actattattt agtgaaatga
       61 gatattatga tattttctga attgtgatta aaaaggcaac tttatgccca tgcaacagaa
      121 actataaaaa atacagagaa tgaaaagaaa cagatagatt ttttagttct ttaggcccgt
      181 agtctgcaaa tccttttatg attttctatc aaacaaaaga ggaaaataga ccagttgcaa
      241 tccaaacgag agtctaatag aatgaggtcg aaaagtaaat cgcgcgggtt tgttactgat
      301 aaagcaggca agacctaaaa tgtgtaaagg gcaaagtgta tactttggcg tcacccctta
      361 catattttag gtcttttttt attgtgcgta actaacttgc catcttcaaa caggagggct
      421 ggaagaagca gaccgctaac acagtacata aaaaaggaga catgaacgat gaacatcaaa
      481 aagtttgcaa aacaagcaac agtattaacc tttactaccg cactgctggc aggaggcgca
      541 actcaagcgt ttgcgaaaga aacgaaccaa aagccatata aggaaacata cggcatttcc
      601 catattacac gccatgatat gctgcaaatc cctgaacagc aaaaaaatga aaaatatcaa
      661 gttcctgagt tcgattcgtc cacaattaaa aatatctctt ctgcaaaagg cctggacgtt
      721 tgggacagct ggccattaca aaacgctgac ggcactgtcg caaactatca cggctaccac
      781 atcgtctttg cattagccgg agatcctaaa aatgcggatg acacatcgat ttacatgttc
      841 tatcaaaaag tcggcgaaac ttctattgac agctggaaaa acgctggccg cgtctttaaa
      901 gacagcgaca aattcgatgc aaatgattct atcctaaaag accaaacaca agaatggtca
      961 ggttcagcca catttacatc tgacggaaaa atccgtttat tctacactga tttctccggt
     1021 aaacattacg gcaaacaaac actgacaact gcacaagtta acgtatcagc atcagacagc
     1081 tctttgaaca tcaacggtgt agaggattat aaatcaatct ttgacggtga cggaaaaacg
     1141 tatcaaaatg tacagcagtt catcgatgaa ggcaactaca gctcaggcga caaccatacg
     1201 ctgagagatc ctcactacgt agaagataaa ggccacaaat acttagtatt tgaagcaaac
     1261 actggaactg aagatggcta ccaaggcgaa gaatctttat ttaacaaagc atactatggc
     1321 aaaagcacat cattcttccg tcaagaaagt caaaaacttc tgcaaagcga taaaaaacgc
     1381 acggctgagt tagcaaacgg cgctctcggt atgattgagc taaacgatga ttacacactg
     1441 aaaaaagtga tgaaaccgct gattgcatct aacacagtaa cagatgaaat tgaacgcgcg
     1501 aacgtcttta aaatgaacgg caaatggtac ctgttcactg actcccgcgg atcaaaaatg
     1561 acgattgacg gcattacgtc taacgatatt tacatgcttg gttatgtttc taattcttta
     1621 actggcccat acaagccgct gaacaaaact ggccttgtgt taaaaatgga tcttgatcct
     1681 aacgatgtaa cctttactta ctcacacttc gctgtacctc aagcgaaagg aaacaatgtc
     1741 gtgattacaa gctatatgac aaacagagga ttctacgcag acaaacaatc aacgtttgcg
     1801 ccgagcttcc tgctgaacat caaaggcaag aaaacatctg ttgtcaaaga cagcatcctt
     1861 gaacaaggac aattaacagt taacaaataa cacctgcagc tgtattgaga ccgagctcct
     1921 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     1981 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     2041 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     2101 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc
     2161 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     2221 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     2281 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt
     2341 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     2401 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     2461 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
     2521 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc
     2581 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     2641 cgaggggaaa ttaggccggg aagccgatct cggcttgaac gaattgttag gtggcggtac
     2701 ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca
     2761 ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg
     2821 cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg
     2881 agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacctg ggcagaacgt
     2941 aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct
     3001 tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta
     3061 cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag
     3121 ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc
     3181 gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct ccataacatc
     3241 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca
     3301 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     3361 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc
     3421 gaacaggctt atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg
     3481 gcaaccttgg gcagcagcga agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag
     3541 gtttcgctct ccacgcatcg tcaggcattg gcggccttgc tgttcttcta cggcaaggtg
     3601 ctgtgcacgg atctgccctg gcttcaggag atcggaagac ctcggccgtc gcggcgcttg
     3661 ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg gttttctgga aggcgagcat
     3721 cgtttgttcg cccaggactc tagctatagt tctagtggtt ggctactaat aggttgtatt
     3781 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     3841 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     3901 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     3961 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     4021 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4081 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4141 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4201 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     4261 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4321 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4381 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4441 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4501 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4561 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4621 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4681 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4741 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgct agccaggaag
     4801 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     4861 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     4921 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     4981 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     5041 ctctcgcgtt aacgctagca tggatctcgg gccc
//
