LOCUS       pFASTR-pGATA23-Cas9-P2A-mCherry-G7T-AtU6-CDKA1-1,CDKB1-1       17500 bp    DNA     circular UNA 10-FEB-2023
DEFINITION  .
ACCESSION   p00811
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     Promoter        3..277
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    278..1069
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1315..1903
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     regulatory      complement(1939..2199)
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /Alternative_Match="99.29%; Adjusted-Interval-0:30/150; <a
                     href=""urn:local:.:79-dlucaql"">bom</a>"
                     /label="pBR322 bom"
     rep_origin      2573..2767
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     regulatory      complement(2833..3906)
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     regulatory      complement(4335..4964)
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     T-DNA_border    6150..6380
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6f-dgycryw"">RB</a>"
                     /Alternative_Match="99.50%; Adjusted-Interval-0:30/0; <a
                     href=""urn:local:.:3c-djznfva"">RB</a>"
                     /label="RB"
     promoter        6386..7257
                     /vntifkey=29
                     /modified_by="wacae"
                     /Transferred_From="pGATA23"
                     /Transferred_Similarity="100.00%"
                     /label="pGATA23"
     UTR             7132..7257
                     /vntifkey=52
                     /note="Unknown feature type:3'_UTR"
                     /modified_by="wacae"
                     /Transferred_From="pGATA23"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="5'UTR"
     CDS             7292..11395
                     /Transferred_From="Cas9PTA"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="AtCAS9"
     NLS             11408..11428
                     /created_by="warddecaestecker"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS SV40"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (<a
                     href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /label="SV40"
     CDS             11444..11500
                     /created_by="User"
                     /X-Transferred_From="P2A"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="P2A"
                     /Transferred_Similarity="100.00%"
                     /label="P2A"
     CDS             11501..12208
                     /created_by="User"
                     /modified_by="User"
                     /X-Transferred_From="mCherry"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /label="mCherry"
     Linker          12212..12259
                     /created_by="wacae"
                     /modified_by="wacae"
                     /Transferred_From="(GS)8"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="Linker"
     NLS             12260..12508
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS N7"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; N7 (<a
                     href=""urn:local:.:9y-dgycryz"">NLS N7</a>)"
                     /label="N7"
     terminator      12516..12787
                     /Transferred_From="G7T"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="tG7"
     promoter        12794..13181
                     /vntifkey=29
                     /modified_by="thjac"
                     /modified_by="wacae"
                     /Transferred_From="AtU6-26"
                     /Transferred_Similarity="100.00%"
                     /label="AtU6-26"
     unsure          13182..13201
                     /created_by="wacae"
                     /label="CDKA-gRNA1"
     Scaffold        13202..13284
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
                     TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
                     /annotation_group="Scaffold: 451 -> 533"
                     /modified_by="wacae"
                     /Transferred_From="Scaffold, gRNA scaffold"
                     /Transferred_Similarity="100.00%"
     promoter        13319..13706
                     /vntifkey=29
                     /modified_by="thjac"
                     /modified_by="wacae"
                     /Transferred_From="AtU6-26"
                     /Transferred_Similarity="100.00%"
                     /label="AtU6-26"
     unsure          13707..13726
                     /created_by="wacae"
                     /modified_by="wacae"
                     /label="CDKB-gRNA1"
     Scaffold        13727..13809
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
                     TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
                     /annotation_group="Scaffold: 451 -> 533"
                     /modified_by="wacae"
                     /Transferred_From="Scaffold, gRNA scaffold"
                     /Transferred_Similarity="100.00%"
     promoter        13824..15266
                     /modified_by="wacae"
                     /Transferred_From="pOLE1"
                     /Transferred_Similarity="100.00%"
                     /label="pOLE1"
     CDS             join(15271..15623,15864..16029)
                     /modified_by="thjac"
                     /X-Transferred_From="OLE1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="OLE1 gene"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="OLE1"
     intron          15624..15863
                     /created_by="wacae"
                     /Transferred_From="OLE1 gene"
                     /Transferred_Similarity="100.00%"
                     /label
     CDS             16036..16746
                     /note="added by Rabuo"
                     /X-Transferred_From="mRuby3"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="wacae"
                     /Transferred_From="mRuby3 without T38V"
                     /Transferred_Similarity="100.00%"
                     /label="mRuby3 without T38V"
     Linker          16751..16796
                     /created_by="wacae"
                     /modified_by="wacae"
                     /Transferred_From="Linker"
                     /Transferred_Similarity="100.00%"
     terminator      16801..17068
                     /Transferred_From="NOST"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="tNOS"
     T-DNA_border    17168..17500
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
ORIGIN      
        1 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
       61 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
      121 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
      181 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
      241 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga
      301 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
      361 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
      421 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
      481 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
      541 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
      601 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
      661 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
      721 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
      781 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
      841 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
      901 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
      961 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     1021 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag
     1081 aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta
     1141 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg
     1201 tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg
     1261 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1321 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1381 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1441 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     1501 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1561 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1621 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1681 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     1741 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     1801 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     1861 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     1921 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     1981 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2041 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     2101 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     2161 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     2221 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     2281 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     2341 tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag
     2401 tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt
     2461 tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga
     2521 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca
     2581 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc
     2641 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg
     2701 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct
     2761 atccacagga aagagagctt ttcgaccttt ttcccctgct agggcaattt gccctagcat
     2821 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat
     2881 gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt
     2941 tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact
     3001 gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc
     3061 caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt
     3121 cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat
     3181 ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc
     3241 ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc
     3301 aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc
     3361 atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg
     3421 cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat
     3481 cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac
     3541 gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag
     3601 acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat
     3661 cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc
     3721 ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc
     3781 accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt
     3841 ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc
     3901 gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc
     3961 cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg
     4021 gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc
     4081 atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt
     4141 aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa
     4201 ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt
     4261 gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc
     4321 tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg
     4381 cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct
     4441 gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc
     4501 gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc
     4561 gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg
     4621 gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg
     4681 atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc
     4741 cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc
     4801 ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact
     4861 aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc
     4921 ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta
     4981 tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact
     5041 caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg
     5101 gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg
     5161 agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt
     5221 cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg
     5281 gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc
     5341 accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag
     5401 ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc
     5461 gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt
     5521 cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg
     5581 ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt
     5641 gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc
     5701 aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac
     5761 gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc
     5821 ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt
     5881 ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct
     5941 cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa
     6001 acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga
     6061 cttgtgcgac atgtcgtttt cagaagaggg ctgcactgaa cgtcagaagc cgactgcact
     6121 atagcagcgg aggggttgga tcaaagtact ttaaagtact ttaaagtact ttaaagtact
     6181 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
     6241 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
     6301 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
     6361 atccaagctc aagctaagct tacctcataa cttttcaata atggatctcg gctattgatt
     6421 gttctagacc aatatgatac atgaataatc tttgcgtaca cctaaatttt atagtgaaaa
     6481 atacataatt ttaaaacttt acttttgtag agatgcctaa aaaaaaaatg gtttaccaca
     6541 tggacattgt tatttgttca gatctccaac gtatagcaat atttgtgatt tatagatatg
     6601 taatttggtg ttcaaagaat gtgtagaaca attattctta tgggtatgcc aagaaaagtt
     6661 atataacttt attctatact gctttcacgt taaataatgt aacattatta atgttgtcct
     6721 tttacttttc cttgaattga tttgggaaga agactattgg cttccttatc acatcaaaaa
     6781 gtctatgagt aagtgaaaca ttggctggct tttgcttcat tcctttgtct ctgtcttcat
     6841 gtgaacaagt ttgcagaaat ttgtggtttc tttttttact ttcttttgtc taatacacac
     6901 actctcacca ctattataaa gaaaatccat agatcccggt cgtgcgtact gccactttgc
     6961 tttccacccg acaatgattt gggagatgtt ttgttttgtg tcatacatct accaaaaaag
     7021 tcttaatcat tgttcatcaa aagagcgccc aagggctctt tcgtcttctt accacacgca
     7081 cactagttcg agagggtttt taaggagctc ttaagccttt cttgatgact cataccacga
     7141 acaagaacca atcttgtcac ccatataatc tccgtattca gagaattgaa gagttcttga
     7201 tcactactgt tgtatcaaaa aggaactcta agaactaaga ttgttttttt ttatttgaac
     7261 agtattcagt cgactggtac caacaggctc gatggataag aagtactcta tcggactcga
     7321 tatcggaact aactctgtgg gatgggctgt gatcaccgat gagtacaagg tgccatctaa
     7381 gaagttcaag gttctcggaa acaccgatag gcactctatc aagaaaaacc ttatcggtgc
     7441 tctcctcttc gattctggtg aaactgctga ggctaccaga ctcaagagaa ccgctagaag
     7501 aaggtacacc agaagaaaga acaggatctg ctacctccaa gagatcttct ctaacgagat
     7561 ggctaaagtg gatgattcat tcttccacag gctcgaagag tcattcctcg tggaagaaga
     7621 taagaagcac gagaggcacc ctatcttcgg aaacatcgtt gatgaggtgg cataccacga
     7681 gaagtaccct actatctacc acctcagaaa gaagctcgtt gattctactg ataaggctga
     7741 tctcaggctc atctacctcg ctctcgctca catgatcaag ttcagaggac acttcctcat
     7801 cgagggtgat ctcaaccctg ataactctga tgtggataag ttgttcatcc agctcgtgca
     7861 gacctacaac cagcttttcg aagagaaccc tatcaacgct tcaggtgtgg atgctaaggc
     7921 tatcctctct gctaggctct ctaagtcaag aaggcttgag aacctcattg ctcagctccc
     7981 tggtgagaag aagaacggac ttttcggaaa cttgatcgct ctctctctcg gactcacccc
     8041 taacttcaag tctaacttcg atctcgctga ggatgcaaag ctccagctct caaaggatac
     8101 ctacgatgat gatctcgata acctcctcgc tcagatcgga gatcagtacg ctgatttgtt
     8161 cctcgctgct aagaacctct ctgatgctat cctcctcagt gatatcctca gagtgaacac
     8221 cgagatcacc aaggctccac tctcagcttc tatgatcaag agatacgatg agcaccacca
     8281 ggatctcaca cttctcaagg ctcttgttag acagcagctc ccagagaagt acaaagagat
     8341 tttcttcgat cagtctaaga acggatacgc tggttacatc gatggtggtg catctcaaga
     8401 agagttctac aagttcatca agcctatcct cgagaagatg gatggaaccg aggaactcct
     8461 cgtgaagctc aatagagagg atcttctcag aaagcagagg accttcgata acggatctat
     8521 ccctcatcag atccacctcg gagagttgca cgctatcctt agaaggcaag aggatttcta
     8581 cccattcctc aaggataaca gggaaaagat tgagaagatt ctcaccttca gaatccctta
     8641 ctacgtggga cctctcgcta gaggaaactc aagattcgct tggatgacca gaaagtctga
     8701 ggaaaccatc accccttgga acttcgaaga ggtggtggat aagggtgcta gtgctcagtc
     8761 tttcatcgag aggatgacca acttcgataa gaaccttcca aacgagaagg tgctccctaa
     8821 gcactctttg ctctacgagt acttcaccgt gtacaacgag ttgaccaagg ttaagtacgt
     8881 gaccgaggga atgaggaagc ctgctttttt gtcaggtgag caaaagaagg ctatcgttga
     8941 tctcttgttc aagaccaaca gaaaggtgac cgtgaagcag ctcaaagagg attacttcaa
     9001 gaaaatcgag tgcttcgatt cagttgagat ttctggtgtt gaggataggt tcaacgcatc
     9061 tctcggaacc taccacgatc tcctcaagat cattaaggat aaggatttct tggataacga
     9121 ggaaaacgag gatatcttgg aggatatcgt tcttaccctc accctctttg aagatagaga
     9181 gatgattgaa gaaaggctca agacctacgc tcatctcttc gatgataagg tgatgaagca
     9241 gttgaagaga agaagataca ctggttgggg aaggctctca agaaagctca ttaacggaat
     9301 cagggataag cagtctggaa agacaatcct tgatttcctc aagtctgatg gattcgctaa
     9361 cagaaacttc atgcagctca tccacgatga ttctctcacc tttaaagagg atatccagaa
     9421 ggctcaggtt tcaggacagg gtgatagtct ccatgagcat atcgctaacc tcgctggatc
     9481 tcctgcaatc aagaagggaa tcctccagac tgtgaaggtt gtggatgagt tggtgaaggt
     9541 gatgggaagg cataagcctg agaacatcgt gatcgaaatg gctagagaga accagaccac
     9601 tcagaaggga cagaagaact ctagggaaag gatgaagagg atcgaggaag gtatcaaaga
     9661 gcttggatct cagatcctca aagagcaccc tgttgagaac actcagctcc agaatgagaa
     9721 gctctacctc tactacctcc agaacggaag ggatatgtat gtggatcaag agttggatat
     9781 caacaggctc tctgattacg atgttgatca tatcgtgcca cagtcattct tgaaggatga
     9841 ttctatcgat aacaaggtgc tcaccaggtc tgataagaac aggggtaaga gtgataacgt
     9901 gccaagtgaa gaggttgtga agaaaatgaa gaactattgg aggcagctcc tcaacgctaa
     9961 gctcatcact cagagaaagt tcgataactt gactaaggct gagaggggag gactctctga
    10021 attggataag gcaggattca tcaagaggca gcttgtggaa accaggcaga tcactaagca
    10081 cgttgcacag atcctcgatt ctaggatgaa caccaagtac gatgagaacg ataagttgat
    10141 cagggaagtg aaggttatca ccctcaagtc aaagctcgtg tctgatttca gaaaggattt
    10201 ccaattctac aaggtgaggg aaatcaacaa ctaccaccac gctcacgatg cttaccttaa
    10261 cgctgttgtt ggaaccgctc tcatcaagaa gtatcctaag ctcgagtcag agttcgtgta
    10321 cggtgattac aaggtgtacg atgtgaggaa gatgatcgct aagtctgagc aagagatcgg
    10381 aaaggctacc gctaagtatt tcttctactc taacatcatg aatttcttca agaccgagat
    10441 taccctcgct aacggtgaga tcagaaagag gccactcatc gagacaaacg gtgaaacagg
    10501 tgagatcgtg tgggataagg gaagggattt cgctaccgtt agaaaggtgc tctctatgcc
    10561 acaggtgaac atcgttaaga aaaccgaggt gcagaccggt ggattctcta aagagtctat
    10621 cctccctaag aggaactctg ataagctcat tgctaggaag aaggattggg accctaagaa
    10681 atacggtggt ttcgattctc ctaccgtggc ttactctgtt ctcgttgtgg ctaaggttga
    10741 gaagggaaag agtaagaagc tcaagtctgt taaggaactt ctcggaatca ctatcatgga
    10801 aaggtcatct ttcgagaaga acccaatcga tttcctcgag gctaagggat acaaagaggt
    10861 taagaaggat ctcatcatca agctcccaaa gtactcactc ttcgaactcg agaacggtag
    10921 aaagaggatg ctcgcttctg ctggtgagct tcaaaaggga aacgagcttg ctctcccatc
    10981 taagtacgtt aactttcttt acctcgcttc tcactacgag aagttgaagg gatctccaga
    11041 agataacgag cagaagcaac ttttcgttga gcagcacaag cactacttgg atgagatcat
    11101 cgagcagatc tctgagttct ctaaaagggt gatcctcgct gatgcaaacc tcgataaggt
    11161 gttgtctgct tacaacaagc acagagataa gcctatcagg gaacaggcag agaacatcat
    11221 ccatctcttc acccttacca acctcggtgc tcctgctgct ttcaagtact tcgatacaac
    11281 catcgatagg aagagataca cctctaccaa agaagtgctc gatgctaccc tcatccatca
    11341 gtctatcact ggactctacg agactaggat cgatctctca cagctcggtg gtgattcaag
    11401 ggctgatcct aagaagaaga ggaaggtttc agctggatct ggagctacta atttttctct
    11461 tcttaagcaa gctggagatg ttgaagaaaa tcctggaccc atggtgagca agggcgagga
    11521 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
    11581 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
    11641 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
    11701 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
    11761 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
    11821 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
    11881 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agacgatggg
    11941 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
    12001 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
    12061 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
    12121 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
    12181 caccggcggc atggacgagc tgtacaagtc aggttctggt tcaggttcag gttctggatc
    12241 tggaagtgga tctggatcag cggccgcatc tgaattcaag cgtgaagagc aagcaaggaa
    12301 agctaaggtg aacaatgaga aaaagacgga aatagtgaaa ccagagagtt gtagcaatga
    12361 aggagatgtc aaggatctga aaagaaagga ctctgaggat ggaaacgagg gtgaggaaga
    12421 agaagcttct tcgaaaccga aaaagccaaa agttgctctt tctcatcttc aggacattga
    12481 cgacacagaa gctgatcaag aagaagagta actgcccccg atgagctaag ctagctatat
    12541 catcaattta tgtattacac ataatatcgc actcagtctt tcatctacgg caatgtacca
    12601 gctgatataa tcagttattg aaatatttct gaatttaaac ttgcatcaat aaatttatgt
    12661 ttttgcttgg actataatac ctgacttgtt attttatcaa taaatattta aactatattt
    12721 ctttcaagat gggaattaac atctacaaat tgccttttct tatcgaccat gtactcgacg
    12781 gccgagtact actctttttt tcttcttctt cgttcataca gttttttttt gtttatcagc
    12841 ttacattttc ttgaaccgta gctttcgttt tcttcttttt aactttccat tcggagtttt
    12901 tgtatcttgt ttcatagttt gtcccaggat tagaatgatt aggcatcgaa ccttcaagaa
    12961 tttgattgaa taaaacatct tcattcttaa gatatgaaga taatcttcaa aaggcccctg
    13021 ggaatctgaa agaagagaag caggcccatt tatatgggaa agaacaatag tatttcttat
    13081 ataggcccat ttaagttgaa aacaatcttc aaaagtccca catcgcttag ataagaaaac
    13141 gaagctgagt ttatatacag ctagagtcga agtagtgatt ggaagatcag gctagagcag
    13201 ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
    13261 tggcaccgag tcggtgcttt ttttaacagt attcagtcga ctggtaccaa caggctctct
    13321 ttttttcttc ttcttcgttc atacagtttt tttttgttta tcagcttaca ttttcttgaa
    13381 ccgtagcttt cgttttcttc tttttaactt tccattcgga gtttttgtat cttgtttcat
    13441 agtttgtccc aggattagaa tgattaggca tcgaaccttc aagaatttga ttgaataaaa
    13501 catcttcatt cttaagatat gaagataatc ttcaaaaggc ccctgggaat ctgaaagaag
    13561 agaagcaggc ccatttatat gggaaagaac aatagtattt cttatatagg cccatttaag
    13621 ttgaaaacaa tcttcaaaag tcccacatcg cttagataag aaaacgaagc tgagtttata
    13681 tacagctaga gtcgaagtag tgattggctg aaactcagaa tcaccagttt tagagctaga
    13741 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
    13801 gctttttttg tataagctta ccttacttag atcaacacat aaaagttagt aagtgaagaa
    13861 ccacaacaac aacactagat tcatcttcaa gtgtatgtag gtatagtaac atgaacaaga
    13921 acagactcaa gtacaagatc gcatacgaaa atggaaatgg caatgtcact tccacataat
    13981 caaacacgaa tcctcatatc aacaaggcct gagattctaa ctagctcata acaacttagc
    14041 caatagttac ttgagactac caaatgtatg tagaactaaa gactaaggga cagagagttc
    14101 gtctaaacag gtgaatctag tcgttgttat ctaataaaca attcagcccc aaatgcagaa
    14161 cacacataga gctctctatt gattcaaatt acgatctgat actgataacg tctagatttt
    14221 tagggttaaa gcaatcaatc acctgacgat tcaaggtggt tggatcatga cgattccaga
    14281 aaacatcaag caagctctca aagctacact ctttgggatc atactgaact ctaacaacct
    14341 cgttatgtcc cgtagtgcca gtacagacat cctcgtaact cggattgtgc acgatgccat
    14401 ggctataccc aacctcggtc ttggtcacac caggaactct ctggtaagct agctccactc
    14461 cccagaaaca accggcgcca aattgcgcga attgctgacc tgaagacgga acatcatcgt
    14521 cgggtccttg ggcgattgcg gcggaagatg ggtcagcttg ggcttgagga cgagacgcga
    14581 atccgagtct gttgaaaagg ttgttcattg gggatttgta tacggagatt ggtcgtcgag
    14641 aggtttgagg gaaaggacaa atgggtttgg ctctggagaa agagagtgcg gctttagaga
    14701 gagaattgag aggtttagag agagatgcgg cggcgatgag cggaggagag acgacgagga
    14761 cctgcattat caaagcagtg acgtggtgaa atttggaact tttaagaggc agatagattt
    14821 attatttgta tccattttct tcattgttct agaatgtcgc ggaacaaatt ttaaaactaa
    14881 atcctaaatt tttctaattt tgttgccaat agtggatatg tgggccgtat agaaggaatc
    14941 tattgaaggc ccaaacccat actgacgagc ccaaaggttc gttttgcgtt ttatgtttcg
    15001 gttcgatgcc aacgccacat tctgagctag gcaaaaaaca aacgtgtctt tgaatagact
    15061 cctctcgtta acacatgcag cggctgcatg gtgacgccat taacacgtgg cctacaattg
    15121 catgatgtct ccattgacac gtgacttctc gtctcctttc ttaatatatc taacaaacac
    15181 tcctacctct tccaaaatat atacacatct ttttgatcaa tctctcattc aaaatctcat
    15241 tctctctagt aaacaagaac aaaaaaaaca atggcggata cagctagagg aacccatcac
    15301 gatatcatcg gcagagatca gtacccgatg atgggccgag atcgagatca gtaccagatg
    15361 tccggacgag gatctgacta ctccaagtct aggcagattg ctaaagctgc aactgctgtc
    15421 acagctggtg gttccctcct tgttctctcc agccttaccc ttgttggaac tgtcatagct
    15481 ttgactgttg caacacctct gctcgttatc ttcagcccaa tccttgtccc ggctctcatc
    15541 acagttgcac tcctcatcac cggttttctt tcctctggag ggtttggcat tgccgctata
    15601 accgttttct cttggattta caagtaagca cacatttatc atcttacttc ataattttgt
    15661 gcaatatgtg catgcatgtg ttgagccagt agctttggat caattttttt ggtcgaataa
    15721 caaatgtaac aataagaaat tgcaaattct agggaacatt tggttaacta aatacgaaat
    15781 ttgacctagc tagcttgaat gtgtctgtgt atatcatcta tataggtaaa atgcttggta
    15841 tgatacctat tgattgtgaa taggtacgca acgggagagc acccacaggg atcagacaag
    15901 ttggacagtg caaggatgaa gttgggaagc aaagctcagg atctgaaaga cagagctcag
    15961 tactacggac agcaacatac tggtggggaa catgaccgtg accgtactcg tggtggccag
    16021 cacactactg gctccatggt gtctaagggc gaagagctga tcaaggaaaa tatgcgtatg
    16081 aaggtggtca tggaaggttc ggtcaacggc caccaattca aatgcacagg tgaaggagaa
    16141 ggcagaccgt acgagggaac tcaaaccatg aggatcaaag tcatcgaggg aggacccctg
    16201 ccatttgcct ttgacattct tgccacgtcg ttcatgtatg gcagccgtac ttttatcaag
    16261 tacccggccg acatccctga tttctttaaa cagtcctttc ctgagggttt tacttgggaa
    16321 agagttacga gatacgaaga tggtggagtc gtcaccgtca cgcaggacac cagccttgag
    16381 gatggcgagc tcgtctacaa cgtcaaggtc agaggggtaa actttccctc caatggtccc
    16441 gtgatgcaga agaagaccaa gggttgggag cctaatacag agatgatgta tccagcagat
    16501 ggtggtctga gaggatacac tgacatcgca ctgaaagttg atggtggtgg ccatctgcac
    16561 tgcaacttcg tgacaactta caggtcaaaa aagaccgtcg ggaacatcaa gatgcccggt
    16621 gtccatgccg ttgatcaccg cctggaaagg atcgaggaga gtgacaatga aacctacgta
    16681 gtgcaacgcg aagtggcagt tgccaaatac agcaaccttg gtggtggcat ggacgagctg
    16741 tacaagtcag gtggatccta gataaccttt accttcattc cctgtaaatt ggtaccctgc
    16801 ctagagtcaa gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
    16861 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
    16921 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
    16981 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
    17041 cgcggtgtca tctatgttac tagatcgaac tactgctcag ggcttaagct aggtagttcg
    17101 cgtatgcgag tggtatctgc aggcatgcga cgtcgggccc tctagaggat ccccgggtac
    17161 cgcgaattca attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt
    17221 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
    17281 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac
    17341 ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc
    17401 ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat
    17461 gttgattgta acgatgacag agcgttgctg cctgtgatca
//
