LOCUS       pFASTR-pGATA23-Cas9-P2A-mCherry-G7T-AtU6-CDKA1-1,CDKA1-2       17499 bp    DNA     circular UNA 10-FEB-2023
DEFINITION  .
ACCESSION   p00567
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     Promoter        3..277
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    278..1069
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1315..1903
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     regulatory      complement(1939..2199)
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /Alternative_Match="99.29%; Adjusted-Interval-0:30/150; <a
                     href=""urn:local:.:79-dlucaql"">bom</a>"
                     /label="pBR322 bom"
     rep_origin      2573..2767
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     regulatory      complement(2833..3906)
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     regulatory      complement(4335..4964)
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     T-DNA_border    6150..6380
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6f-dgycryw"">RB</a>"
                     /Alternative_Match="99.50%; Adjusted-Interval-0:30/0; <a
                     href=""urn:local:.:3c-djznfva"">RB</a>"
                     /label="RB"
     promoter        6386..7256
                     /vntifkey=29
                     /modified_by="wacae"
                     /Transferred_From="pGATA23"
                     /Transferred_Similarity="99.89%"
                     /label="pGATA23"
     UTR             7131..7256
                     /vntifkey=52
                     /note="Unknown feature type:3'_UTR"
                     /modified_by="wacae"
                     /Transferred_From="pGATA23"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="5'UTR"
     CDS             7291..11394
                     /Transferred_From="Cas9PTA"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="AtCAS9"
     NLS             11407..11427
                     /created_by="warddecaestecker"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS SV40"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (<a
                     href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /label="SV40"
     CDS             11443..11499
                     /created_by="User"
                     /X-Transferred_From="P2A"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="P2A"
                     /Transferred_Similarity="100.00%"
                     /label="P2A"
     CDS             11500..12207
                     /created_by="User"
                     /modified_by="User"
                     /X-Transferred_From="mCherry"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /label="mCherry"
     Linker          12211..12258
                     /created_by="wacae"
                     /modified_by="wacae"
                     /Transferred_From="(GS)8"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="Linker"
     NLS             12259..12507
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS N7"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; N7 (<a
                     href=""urn:local:.:9y-dgycryz"">NLS N7</a>)"
                     /label="N7"
     terminator      12515..12786
                     /Transferred_From="G7T"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="tG7"
     promoter        12793..13180
                     /vntifkey=29
                     /modified_by="thjac"
                     /modified_by="wacae"
                     /Transferred_From="AtU6-26"
                     /Transferred_Similarity="100.00%"
                     /label="AtU6-26"
     unsure          13181..13200
                     /created_by="wacae"
                     /label="CDKA1-1"
     Scaffold        13201..13283
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
                     TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
                     /annotation_group="Scaffold: 451 -> 533"
                     /modified_by="wacae"
                     /Transferred_From="Scaffold, gRNA scaffold"
                     /Transferred_Similarity="100.00%"
     promoter        13318..13705
                     /vntifkey=29
                     /modified_by="thjac"
                     /modified_by="wacae"
                     /Transferred_From="AtU6-26"
                     /Transferred_Similarity="100.00%"
                     /label="AtU6-26"
     unsure          13706..13725
                     /created_by="wacae"
                     /label="CDKA1-2"
     Scaffold        13726..13808
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
                     TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
                     /annotation_group="Scaffold: 451 -> 533"
                     /modified_by="wacae"
                     /Transferred_From="Scaffold, gRNA scaffold"
                     /Transferred_Similarity="100.00%"
     promoter        13823..15265
                     /modified_by="wacae"
                     /Transferred_From="pOLE1"
                     /Transferred_Similarity="100.00%"
                     /label="pOLE1"
     CDS             join(15270..15622,15863..16028)
                     /modified_by="thjac"
                     /X-Transferred_From="OLE1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="OLE1 gene"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="OLE1"
     intron          15623..15862
                     /created_by="wacae"
                     /Transferred_From="OLE1 gene"
                     /Transferred_Similarity="100.00%"
                     /label
     CDS             16035..16745
                     /note="added by Rabuo"
                     /X-Transferred_From="mRuby3"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="wacae"
                     /Transferred_From="mRuby3 without T38V"
                     /Transferred_Similarity="100.00%"
                     /label="mRuby3 without T38V"
     Linker          16750..16795
                     /created_by="wacae"
                     /modified_by="wacae"
                     /Transferred_From="Linker"
                     /Transferred_Similarity="100.00%"
     terminator      16800..17067
                     /Transferred_From="NOST"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="tNOS"
     T-DNA_border    17167..17499
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
ORIGIN      
        1 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
       61 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
      121 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
      181 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
      241 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga
      301 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
      361 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
      421 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
      481 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
      541 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
      601 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
      661 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
      721 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
      781 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
      841 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
      901 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
      961 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     1021 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag
     1081 aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta
     1141 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg
     1201 tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg
     1261 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1321 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1381 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1441 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     1501 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1561 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1621 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1681 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     1741 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     1801 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     1861 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     1921 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     1981 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2041 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     2101 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     2161 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     2221 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     2281 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     2341 tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag
     2401 tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt
     2461 tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga
     2521 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca
     2581 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc
     2641 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg
     2701 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct
     2761 atccacagga aagagagctt ttcgaccttt ttcccctgct agggcaattt gccctagcat
     2821 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat
     2881 gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt
     2941 tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact
     3001 gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc
     3061 caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt
     3121 cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat
     3181 ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc
     3241 ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc
     3301 aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc
     3361 atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg
     3421 cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat
     3481 cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac
     3541 gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag
     3601 acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat
     3661 cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc
     3721 ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc
     3781 accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt
     3841 ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc
     3901 gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc
     3961 cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg
     4021 gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc
     4081 atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt
     4141 aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa
     4201 ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt
     4261 gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc
     4321 tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg
     4381 cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct
     4441 gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc
     4501 gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc
     4561 gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg
     4621 gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg
     4681 atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc
     4741 cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc
     4801 ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact
     4861 aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc
     4921 ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta
     4981 tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact
     5041 caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg
     5101 gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg
     5161 agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt
     5221 cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg
     5281 gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc
     5341 accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag
     5401 ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc
     5461 gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt
     5521 cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg
     5581 ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt
     5641 gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc
     5701 aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac
     5761 gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc
     5821 ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt
     5881 ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct
     5941 cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa
     6001 acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga
     6061 cttgtgcgac atgtcgtttt cagaagaggg ctgcactgaa cgtcagaagc cgactgcact
     6121 atagcagcgg aggggttgga tcaaagtact ttaaagtact ttaaagtact ttaaagtact
     6181 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
     6241 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
     6301 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
     6361 atccaagctc aagctaagct tacctcataa cttttcaata atggatctcg gctattgatt
     6421 gttctagacc aatatgatac atgaataatc tttgcgtaca cctaaatttt atagtgaaaa
     6481 atacataatt ttaaaacttt acttttgtag agatgcctaa aaaaaaatgg tttaccacat
     6541 ggacattgtt atttgttcag atctccaacg tatagcaata tttgtgattt atagatatgt
     6601 aatttggtgt tcaaagaatg tgtagaacaa ttattcttat gggtatgcca agaaaagtta
     6661 tataacttta ttctatactg ctttcacgtt aaataatgta acattattaa tgttgtcctt
     6721 ttacttttcc ttgaattgat ttgggaagaa gactattggc ttccttatca catcaaaaag
     6781 tctatgagta agtgaaacat tggctggctt ttgcttcatt cctttgtctc tgtcttcatg
     6841 tgaacaagtt tgcagaaatt tgtggtttct ttttttactt tcttttgtct aatacacaca
     6901 ctctcaccac tattataaag aaaatccata gatcccggtc gtgcgtactg ccactttgct
     6961 ttccacccga caatgatttg ggagatgttt tgttttgtgt catacatcta ccaaaaaagt
     7021 cttaatcatt gttcatcaaa agagcgccca agggctcttt cgtcttctta ccacacgcac
     7081 actagttcga gagggttttt aaggagctct taagcctttc ttgatgactc ataccacgaa
     7141 caagaaccaa tcttgtcacc catataatct ccgtattcag agaattgaag agttcttgat
     7201 cactactgtt gtatcaaaaa ggaactctaa gaactaagat tgtttttttt tatttgaaca
     7261 gtattcagtc gactggtacc aacaggctcg atggataaga agtactctat cggactcgat
     7321 atcggaacta actctgtggg atgggctgtg atcaccgatg agtacaaggt gccatctaag
     7381 aagttcaagg ttctcggaaa caccgatagg cactctatca agaaaaacct tatcggtgct
     7441 ctcctcttcg attctggtga aactgctgag gctaccagac tcaagagaac cgctagaaga
     7501 aggtacacca gaagaaagaa caggatctgc tacctccaag agatcttctc taacgagatg
     7561 gctaaagtgg atgattcatt cttccacagg ctcgaagagt cattcctcgt ggaagaagat
     7621 aagaagcacg agaggcaccc tatcttcgga aacatcgttg atgaggtggc ataccacgag
     7681 aagtacccta ctatctacca cctcagaaag aagctcgttg attctactga taaggctgat
     7741 ctcaggctca tctacctcgc tctcgctcac atgatcaagt tcagaggaca cttcctcatc
     7801 gagggtgatc tcaaccctga taactctgat gtggataagt tgttcatcca gctcgtgcag
     7861 acctacaacc agcttttcga agagaaccct atcaacgctt caggtgtgga tgctaaggct
     7921 atcctctctg ctaggctctc taagtcaaga aggcttgaga acctcattgc tcagctccct
     7981 ggtgagaaga agaacggact tttcggaaac ttgatcgctc tctctctcgg actcacccct
     8041 aacttcaagt ctaacttcga tctcgctgag gatgcaaagc tccagctctc aaaggatacc
     8101 tacgatgatg atctcgataa cctcctcgct cagatcggag atcagtacgc tgatttgttc
     8161 ctcgctgcta agaacctctc tgatgctatc ctcctcagtg atatcctcag agtgaacacc
     8221 gagatcacca aggctccact ctcagcttct atgatcaaga gatacgatga gcaccaccag
     8281 gatctcacac ttctcaaggc tcttgttaga cagcagctcc cagagaagta caaagagatt
     8341 ttcttcgatc agtctaagaa cggatacgct ggttacatcg atggtggtgc atctcaagaa
     8401 gagttctaca agttcatcaa gcctatcctc gagaagatgg atggaaccga ggaactcctc
     8461 gtgaagctca atagagagga tcttctcaga aagcagagga ccttcgataa cggatctatc
     8521 cctcatcaga tccacctcgg agagttgcac gctatcctta gaaggcaaga ggatttctac
     8581 ccattcctca aggataacag ggaaaagatt gagaagattc tcaccttcag aatcccttac
     8641 tacgtgggac ctctcgctag aggaaactca agattcgctt ggatgaccag aaagtctgag
     8701 gaaaccatca ccccttggaa cttcgaagag gtggtggata agggtgctag tgctcagtct
     8761 ttcatcgaga ggatgaccaa cttcgataag aaccttccaa acgagaaggt gctccctaag
     8821 cactctttgc tctacgagta cttcaccgtg tacaacgagt tgaccaaggt taagtacgtg
     8881 accgagggaa tgaggaagcc tgcttttttg tcaggtgagc aaaagaaggc tatcgttgat
     8941 ctcttgttca agaccaacag aaaggtgacc gtgaagcagc tcaaagagga ttacttcaag
     9001 aaaatcgagt gcttcgattc agttgagatt tctggtgttg aggataggtt caacgcatct
     9061 ctcggaacct accacgatct cctcaagatc attaaggata aggatttctt ggataacgag
     9121 gaaaacgagg atatcttgga ggatatcgtt cttaccctca ccctctttga agatagagag
     9181 atgattgaag aaaggctcaa gacctacgct catctcttcg atgataaggt gatgaagcag
     9241 ttgaagagaa gaagatacac tggttgggga aggctctcaa gaaagctcat taacggaatc
     9301 agggataagc agtctggaaa gacaatcctt gatttcctca agtctgatgg attcgctaac
     9361 agaaacttca tgcagctcat ccacgatgat tctctcacct ttaaagagga tatccagaag
     9421 gctcaggttt caggacaggg tgatagtctc catgagcata tcgctaacct cgctggatct
     9481 cctgcaatca agaagggaat cctccagact gtgaaggttg tggatgagtt ggtgaaggtg
     9541 atgggaaggc ataagcctga gaacatcgtg atcgaaatgg ctagagagaa ccagaccact
     9601 cagaagggac agaagaactc tagggaaagg atgaagagga tcgaggaagg tatcaaagag
     9661 cttggatctc agatcctcaa agagcaccct gttgagaaca ctcagctcca gaatgagaag
     9721 ctctacctct actacctcca gaacggaagg gatatgtatg tggatcaaga gttggatatc
     9781 aacaggctct ctgattacga tgttgatcat atcgtgccac agtcattctt gaaggatgat
     9841 tctatcgata acaaggtgct caccaggtct gataagaaca ggggtaagag tgataacgtg
     9901 ccaagtgaag aggttgtgaa gaaaatgaag aactattgga ggcagctcct caacgctaag
     9961 ctcatcactc agagaaagtt cgataacttg actaaggctg agaggggagg actctctgaa
    10021 ttggataagg caggattcat caagaggcag cttgtggaaa ccaggcagat cactaagcac
    10081 gttgcacaga tcctcgattc taggatgaac accaagtacg atgagaacga taagttgatc
    10141 agggaagtga aggttatcac cctcaagtca aagctcgtgt ctgatttcag aaaggatttc
    10201 caattctaca aggtgaggga aatcaacaac taccaccacg ctcacgatgc ttaccttaac
    10261 gctgttgttg gaaccgctct catcaagaag tatcctaagc tcgagtcaga gttcgtgtac
    10321 ggtgattaca aggtgtacga tgtgaggaag atgatcgcta agtctgagca agagatcgga
    10381 aaggctaccg ctaagtattt cttctactct aacatcatga atttcttcaa gaccgagatt
    10441 accctcgcta acggtgagat cagaaagagg ccactcatcg agacaaacgg tgaaacaggt
    10501 gagatcgtgt gggataaggg aagggatttc gctaccgtta gaaaggtgct ctctatgcca
    10561 caggtgaaca tcgttaagaa aaccgaggtg cagaccggtg gattctctaa agagtctatc
    10621 ctccctaaga ggaactctga taagctcatt gctaggaaga aggattggga ccctaagaaa
    10681 tacggtggtt tcgattctcc taccgtggct tactctgttc tcgttgtggc taaggttgag
    10741 aagggaaaga gtaagaagct caagtctgtt aaggaacttc tcggaatcac tatcatggaa
    10801 aggtcatctt tcgagaagaa cccaatcgat ttcctcgagg ctaagggata caaagaggtt
    10861 aagaaggatc tcatcatcaa gctcccaaag tactcactct tcgaactcga gaacggtaga
    10921 aagaggatgc tcgcttctgc tggtgagctt caaaagggaa acgagcttgc tctcccatct
    10981 aagtacgtta actttcttta cctcgcttct cactacgaga agttgaaggg atctccagaa
    11041 gataacgagc agaagcaact tttcgttgag cagcacaagc actacttgga tgagatcatc
    11101 gagcagatct ctgagttctc taaaagggtg atcctcgctg atgcaaacct cgataaggtg
    11161 ttgtctgctt acaacaagca cagagataag cctatcaggg aacaggcaga gaacatcatc
    11221 catctcttca cccttaccaa cctcggtgct cctgctgctt tcaagtactt cgatacaacc
    11281 atcgatagga agagatacac ctctaccaaa gaagtgctcg atgctaccct catccatcag
    11341 tctatcactg gactctacga gactaggatc gatctctcac agctcggtgg tgattcaagg
    11401 gctgatccta agaagaagag gaaggtttca gctggatctg gagctactaa tttttctctt
    11461 cttaagcaag ctggagatgt tgaagaaaat cctggaccca tggtgagcaa gggcgaggag
    11521 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
    11581 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
    11641 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
    11701 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
    11761 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
    11821 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
    11881 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gacgatgggc
    11941 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
    12001 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
    12061 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
    12121 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
    12181 accggcggca tggacgagct gtacaagtca ggttctggtt caggttcagg ttctggatct
    12241 ggaagtggat ctggatcagc ggccgcatct gaattcaagc gtgaagagca agcaaggaaa
    12301 gctaaggtga acaatgagaa aaagacggaa atagtgaaac cagagagttg tagcaatgaa
    12361 ggagatgtca aggatctgaa aagaaaggac tctgaggatg gaaacgaggg tgaggaagaa
    12421 gaagcttctt cgaaaccgaa aaagccaaaa gttgctcttt ctcatcttca ggacattgac
    12481 gacacagaag ctgatcaaga agaagagtaa ctgcccccga tgagctaagc tagctatatc
    12541 atcaatttat gtattacaca taatatcgca ctcagtcttt catctacggc aatgtaccag
    12601 ctgatataat cagttattga aatatttctg aatttaaact tgcatcaata aatttatgtt
    12661 tttgcttgga ctataatacc tgacttgtta ttttatcaat aaatatttaa actatatttc
    12721 tttcaagatg ggaattaaca tctacaaatt gccttttctt atcgaccatg tactcgacgg
    12781 ccgagtacta ctcttttttt cttcttcttc gttcatacag tttttttttg tttatcagct
    12841 tacattttct tgaaccgtag ctttcgtttt cttcttttta actttccatt cggagttttt
    12901 gtatcttgtt tcatagtttg tcccaggatt agaatgatta ggcatcgaac cttcaagaat
    12961 ttgattgaat aaaacatctt cattcttaag atatgaagat aatcttcaaa aggcccctgg
    13021 gaatctgaaa gaagagaagc aggcccattt atatgggaaa gaacaatagt atttcttata
    13081 taggcccatt taagttgaaa acaatcttca aaagtcccac atcgcttaga taagaaaacg
    13141 aagctgagtt tatatacagc tagagtcgaa gtagtgattg gaagatcagg ctagagcagg
    13201 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
    13261 ggcaccgagt cggtgctttt tttaacagta ttcagtcgac tggtaccaac aggctctctt
    13321 tttttcttct tcttcgttca tacagttttt ttttgtttat cagcttacat tttcttgaac
    13381 cgtagctttc gttttcttct ttttaacttt ccattcggag tttttgtatc ttgtttcata
    13441 gtttgtccca ggattagaat gattaggcat cgaaccttca agaatttgat tgaataaaac
    13501 atcttcattc ttaagatatg aagataatct tcaaaaggcc cctgggaatc tgaaagaaga
    13561 gaagcaggcc catttatatg ggaaagaaca atagtatttc ttatataggc ccatttaagt
    13621 tgaaaacaat cttcaaaagt cccacatcgc ttagataaga aaacgaagct gagtttatat
    13681 acagctagag tcgaagtagt gattggctac acagattgca ggatggtttt agagctagaa
    13741 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
    13801 ctttttttgt ataagcttac cttacttaga tcaacacata aaagttagta agtgaagaac
    13861 cacaacaaca acactagatt catcttcaag tgtatgtagg tatagtaaca tgaacaagaa
    13921 cagactcaag tacaagatcg catacgaaaa tggaaatggc aatgtcactt ccacataatc
    13981 aaacacgaat cctcatatca acaaggcctg agattctaac tagctcataa caacttagcc
    14041 aatagttact tgagactacc aaatgtatgt agaactaaag actaagggac agagagttcg
    14101 tctaaacagg tgaatctagt cgttgttatc taataaacaa ttcagcccca aatgcagaac
    14161 acacatagag ctctctattg attcaaatta cgatctgata ctgataacgt ctagattttt
    14221 agggttaaag caatcaatca cctgacgatt caaggtggtt ggatcatgac gattccagaa
    14281 aacatcaagc aagctctcaa agctacactc tttgggatca tactgaactc taacaacctc
    14341 gttatgtccc gtagtgccag tacagacatc ctcgtaactc ggattgtgca cgatgccatg
    14401 gctataccca acctcggtct tggtcacacc aggaactctc tggtaagcta gctccactcc
    14461 ccagaaacaa ccggcgccaa attgcgcgaa ttgctgacct gaagacggaa catcatcgtc
    14521 gggtccttgg gcgattgcgg cggaagatgg gtcagcttgg gcttgaggac gagacgcgaa
    14581 tccgagtctg ttgaaaaggt tgttcattgg ggatttgtat acggagattg gtcgtcgaga
    14641 ggtttgaggg aaaggacaaa tgggtttggc tctggagaaa gagagtgcgg ctttagagag
    14701 agaattgaga ggtttagaga gagatgcggc ggcgatgagc ggaggagaga cgacgaggac
    14761 ctgcattatc aaagcagtga cgtggtgaaa tttggaactt ttaagaggca gatagattta
    14821 ttatttgtat ccattttctt cattgttcta gaatgtcgcg gaacaaattt taaaactaaa
    14881 tcctaaattt ttctaatttt gttgccaata gtggatatgt gggccgtata gaaggaatct
    14941 attgaaggcc caaacccata ctgacgagcc caaaggttcg ttttgcgttt tatgtttcgg
    15001 ttcgatgcca acgccacatt ctgagctagg caaaaaacaa acgtgtcttt gaatagactc
    15061 ctctcgttaa cacatgcagc ggctgcatgg tgacgccatt aacacgtggc ctacaattgc
    15121 atgatgtctc cattgacacg tgacttctcg tctcctttct taatatatct aacaaacact
    15181 cctacctctt ccaaaatata tacacatctt tttgatcaat ctctcattca aaatctcatt
    15241 ctctctagta aacaagaaca aaaaaaacaa tggcggatac agctagagga acccatcacg
    15301 atatcatcgg cagagatcag tacccgatga tgggccgaga tcgagatcag taccagatgt
    15361 ccggacgagg atctgactac tccaagtcta ggcagattgc taaagctgca actgctgtca
    15421 cagctggtgg ttccctcctt gttctctcca gccttaccct tgttggaact gtcatagctt
    15481 tgactgttgc aacacctctg ctcgttatct tcagcccaat ccttgtcccg gctctcatca
    15541 cagttgcact cctcatcacc ggttttcttt cctctggagg gtttggcatt gccgctataa
    15601 ccgttttctc ttggatttac aagtaagcac acatttatca tcttacttca taattttgtg
    15661 caatatgtgc atgcatgtgt tgagccagta gctttggatc aatttttttg gtcgaataac
    15721 aaatgtaaca ataagaaatt gcaaattcta gggaacattt ggttaactaa atacgaaatt
    15781 tgacctagct agcttgaatg tgtctgtgta tatcatctat ataggtaaaa tgcttggtat
    15841 gatacctatt gattgtgaat aggtacgcaa cgggagagca cccacaggga tcagacaagt
    15901 tggacagtgc aaggatgaag ttgggaagca aagctcagga tctgaaagac agagctcagt
    15961 actacggaca gcaacatact ggtggggaac atgaccgtga ccgtactcgt ggtggccagc
    16021 acactactgg ctccatggtg tctaagggcg aagagctgat caaggaaaat atgcgtatga
    16081 aggtggtcat ggaaggttcg gtcaacggcc accaattcaa atgcacaggt gaaggagaag
    16141 gcagaccgta cgagggaact caaaccatga ggatcaaagt catcgaggga ggacccctgc
    16201 catttgcctt tgacattctt gccacgtcgt tcatgtatgg cagccgtact tttatcaagt
    16261 acccggccga catccctgat ttctttaaac agtcctttcc tgagggtttt acttgggaaa
    16321 gagttacgag atacgaagat ggtggagtcg tcaccgtcac gcaggacacc agccttgagg
    16381 atggcgagct cgtctacaac gtcaaggtca gaggggtaaa ctttccctcc aatggtcccg
    16441 tgatgcagaa gaagaccaag ggttgggagc ctaatacaga gatgatgtat ccagcagatg
    16501 gtggtctgag aggatacact gacatcgcac tgaaagttga tggtggtggc catctgcact
    16561 gcaacttcgt gacaacttac aggtcaaaaa agaccgtcgg gaacatcaag atgcccggtg
    16621 tccatgccgt tgatcaccgc ctggaaagga tcgaggagag tgacaatgaa acctacgtag
    16681 tgcaacgcga agtggcagtt gccaaataca gcaaccttgg tggtggcatg gacgagctgt
    16741 acaagtcagg tggatcctag ataaccttta ccttcattcc ctgtaaattg gtaccctgcc
    16801 tagagtcaag cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
    16861 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
    16921 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
    16981 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
    17041 gcggtgtcat ctatgttact agatcgaact actgctcagg gcttaagcta ggtagttcgc
    17101 gtatgcgagt ggtatctgca ggcatgcgac gtcgggccct ctagaggatc cccgggtacc
    17161 gcgaattcaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt
    17221 gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc
    17281 ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg
    17341 gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg
    17401 gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg
    17461 ttgattgtaa cgatgacaga gcgttgctgc ctgtgatca
//
