LOCUS       pFASTR-pPcUBI-Cas9-P2A-mCherry-G7T-AtU6-CDKA1-1,CDKA1-2       17600 bp    DNA     circular UNA 10-FEB-2023
DEFINITION  .
ACCESSION   p00465
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     Promoter        3..277
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    278..1069
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1315..1903
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     regulatory      complement(1939..2199)
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /Alternative_Match="99.29%; Adjusted-Interval-0:30/150; <a
                     href=""urn:local:.:79-dlucaql"">bom</a>"
                     /label="pBR322 bom"
     rep_origin      2573..2767
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     regulatory      complement(2833..3906)
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     regulatory      complement(4335..4964)
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     T-DNA_border    6150..6380
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6f-dgycryw"">RB</a>"
                     /Alternative_Match="99.50%; Adjusted-Interval-0:30/0; <a
                     href=""urn:local:.:3c-djznfva"">RB</a>"
                     /label="RB"
     promoter        6386..7357
                     /vntifkey=29
                     /modified_by="wacae"
                     /Transferred_From="pPcUBI"
                     /Transferred_Similarity="100.00%"
                     /label="pPcUBI"
     CDS             7392..11495
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AtCas9"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:95-dgycryy"">AtCas9</a>"
                     /label="AtCAS9"
     NLS             11508..11528
                     /created_by="warddecaestecker"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS SV40"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (<a
                     href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /label="SV40"
     CDS             11544..11600
                     /created_by="User"
                     /X-Transferred_From="P2A"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="P2A"
                     /Transferred_Similarity="100.00%"
                     /label="P2A"
     CDS             11601..12308
                     /created_by="User"
                     /modified_by="User"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:93-dgycryy"">mCherry</a>"
                     /label="mCherry"
     Linker          12312..12359
                     /created_by="wacae"
                     /modified_by="wacae"
                     /Transferred_From="(GS)8"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="Linker"
     NLS             12360..12608
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS N7"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; N7 (<a
                     href=""urn:local:.:9y-dgycryz"">NLS N7</a>)"
                     /label="N7"
     terminator      12616..12887
                     /Transferred_From="G7T"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="tG7"
     promoter        12894..13281
                     /vntifkey=29
                     /modified_by="thjac"
                     /modified_by="wacae"
                     /Transferred_From="AtU6-26"
                     /Transferred_Similarity="100.00%"
                     /label="AtU6-26"
     unsure          13282..13301
                     /created_by="wacae"
                     /label="CDKA1-1"
     Scaffold        13302..13384
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
                     TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
                     /annotation_group="Scaffold: 451 -> 533"
                     /modified_by="wacae"
                     /Transferred_From="Scaffold, gRNA scaffold"
                     /Transferred_Similarity="100.00%"
     promoter        13419..13806
                     /vntifkey=29
                     /modified_by="thjac"
                     /modified_by="wacae"
                     /Transferred_From="AtU6-26"
                     /Transferred_Similarity="100.00%"
                     /label="AtU6-26"
     unsure          13807..13826
                     /created_by="wacae"
                     /label="CDKA1-2"
     Scaffold        13827..13909
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
                     TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
                     /annotation_group="Scaffold: 451 -> 533"
                     /modified_by="wacae"
                     /Transferred_From="Scaffold, gRNA scaffold"
                     /Transferred_Similarity="100.00%"
     promoter        13924..15366
                     /modified_by="wacae"
                     /Transferred_From="pOLE1"
                     /Transferred_Similarity="100.00%"
                     /label="pOLE1"
     CDS             join(15371..15723,15964..16129)
                     /modified_by="thjac"
                     /X-Transferred_From="OLE1"
                     /X-Transferred_Similarity="100.00%"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="OLE1 gene"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0,
                     Adjusted-Interval-1:0/0; OLE1 (<a
                     href=""urn:local:.:91-dgycryy"">OLE1 gene</a>)"
                     /label="OLE1"
     intron          15724..15963
                     /created_by="wacae"
                     /Transferred_From="OLE1 gene"
                     /Transferred_Similarity="100.00%"
                     /label
     CDS             16136..16846
                     /note="added by Rabuo"
                     /X-Transferred_From="mRuby3"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="wacae"
                     /Transferred_From="mRuby3 without T38V"
                     /Transferred_Similarity="100.00%"
                     /label="mRuby3 without T38V"
     Linker          16851..16896
                     /created_by="wacae"
                     /modified_by="wacae"
                     /Transferred_From="Linker"
                     /Transferred_Similarity="100.00%"
     terminator      16901..17168
                     /Transferred_From="NOST"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="tNOS"
     T-DNA_border    17268..17600
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
ORIGIN      
        1 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
       61 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
      121 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
      181 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
      241 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga
      301 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
      361 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
      421 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
      481 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
      541 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
      601 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
      661 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
      721 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
      781 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
      841 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
      901 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
      961 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     1021 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag
     1081 aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta
     1141 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg
     1201 tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg
     1261 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1321 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1381 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1441 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     1501 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1561 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1621 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1681 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     1741 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     1801 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     1861 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     1921 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     1981 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2041 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     2101 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     2161 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     2221 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     2281 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     2341 tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag
     2401 tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt
     2461 tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga
     2521 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca
     2581 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc
     2641 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg
     2701 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct
     2761 atccacagga aagagagctt ttcgaccttt ttcccctgct agggcaattt gccctagcat
     2821 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat
     2881 gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt
     2941 tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact
     3001 gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc
     3061 caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt
     3121 cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat
     3181 ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc
     3241 ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc
     3301 aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc
     3361 atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg
     3421 cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat
     3481 cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac
     3541 gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag
     3601 acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat
     3661 cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc
     3721 ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc
     3781 accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt
     3841 ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc
     3901 gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc
     3961 cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg
     4021 gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc
     4081 atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt
     4141 aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa
     4201 ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt
     4261 gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc
     4321 tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg
     4381 cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct
     4441 gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc
     4501 gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc
     4561 gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg
     4621 gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg
     4681 atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc
     4741 cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc
     4801 ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact
     4861 aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc
     4921 ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta
     4981 tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact
     5041 caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg
     5101 gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg
     5161 agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt
     5221 cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg
     5281 gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc
     5341 accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag
     5401 ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc
     5461 gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt
     5521 cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg
     5581 ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt
     5641 gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc
     5701 aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac
     5761 gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc
     5821 ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt
     5881 ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct
     5941 cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa
     6001 acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga
     6061 cttgtgcgac atgtcgtttt cagaagaggg ctgcactgaa cgtcagaagc cgactgcact
     6121 atagcagcgg aggggttgga tcaaagtact ttaaagtact ttaaagtact ttaaagtact
     6181 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
     6241 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
     6301 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
     6361 atccaagctc aagctaagct tacctaaaaa ttacggatat gaatataggc atatccgtat
     6421 ccgaattatc cgtttgacag ctagcaacga ttgtacaatt gcttctttaa aaaaggaaga
     6481 aagaaagaaa gaaaagaatc aacatcagcg ttaacaaacg gccccgttac ggcccaaacg
     6541 gtcatataga gtaacggcgt taagcgttga aagactccta tcgaaatacg taaccgcaaa
     6601 cgtgtcatag tcagatcccc tcttccttca ccgcctcaaa cacaaaaata atcttctaca
     6661 gcctatatat acaacccccc cttctatctc tcctttctca caattcatca tctttctttc
     6721 tctaccccca attttaagaa atcctctctt ctcctcttca ttttcaaggt aaatctctct
     6781 ctctctctct ctctctgtta ttccttgttt taattaggta tgtattattg ctagtttgtt
     6841 aatctgctta tcttatgtat gccttatgtg aatatcttta tcttgttcat ctcatccgtt
     6901 tagaagctat aaatttgttg atttgactgt gtatctacac gtggttatgt ttatatctaa
     6961 tcagatatga atttcttcat attgttgcgt ttgtgtgtac caatccgaaa tcgttgattt
     7021 ttttcattta atcgtgtagc taattgtacg tatacatatg gatctacgta tcaattgttc
     7081 atctgtttgt gtttgtatgt atacagatct gaaaacatca cttctctcat ctgattgtgt
     7141 tgttacatac atagatatag atctgttata tcattttttt tattaattgt gtatatatat
     7201 atgtgcatag atctggatta catgattgtg attatttaca tgattttgtt atttacgtat
     7261 gtatatatgt agatctggac tttttggagt tgttgacttg attgtatttg tgtgtgtata
     7321 tgtgtgttct gatcttgata tgttatgtat gtgcagcaac agtattcagt cgactggtac
     7381 caacaggctc gatggataag aagtactcta tcggactcga tatcggaact aactctgtgg
     7441 gatgggctgt gatcaccgat gagtacaagg tgccatctaa gaagttcaag gttctcggaa
     7501 acaccgatag gcactctatc aagaaaaacc ttatcggtgc tctcctcttc gattctggtg
     7561 aaactgctga ggctaccaga ctcaagagaa ccgctagaag aaggtacacc agaagaaaga
     7621 acaggatctg ctacctccaa gagatcttct ctaacgagat ggctaaagtg gatgattcat
     7681 tcttccacag gctcgaagag tcattcctcg tggaagaaga taagaagcac gagaggcacc
     7741 ctatcttcgg aaacatcgtt gatgaggtgg cataccacga gaagtaccct actatctacc
     7801 acctcagaaa gaagctcgtt gattctactg ataaggctga tctcaggctc atctacctcg
     7861 ctctcgctca catgatcaag ttcagaggac acttcctcat cgagggtgat ctcaaccctg
     7921 ataactctga tgtggataag ttgttcatcc agctcgtgca gacctacaac cagcttttcg
     7981 aagagaaccc tatcaacgct tcaggtgtgg atgctaaggc tatcctctct gctaggctct
     8041 ctaagtcaag aaggcttgag aacctcattg ctcagctccc tggtgagaag aagaacggac
     8101 ttttcggaaa cttgatcgct ctctctctcg gactcacccc taacttcaag tctaacttcg
     8161 atctcgctga ggatgcaaag ctccagctct caaaggatac ctacgatgat gatctcgata
     8221 acctcctcgc tcagatcgga gatcagtacg ctgatttgtt cctcgctgct aagaacctct
     8281 ctgatgctat cctcctcagt gatatcctca gagtgaacac cgagatcacc aaggctccac
     8341 tctcagcttc tatgatcaag agatacgatg agcaccacca ggatctcaca cttctcaagg
     8401 ctcttgttag acagcagctc ccagagaagt acaaagagat tttcttcgat cagtctaaga
     8461 acggatacgc tggttacatc gatggtggtg catctcaaga agagttctac aagttcatca
     8521 agcctatcct cgagaagatg gatggaaccg aggaactcct cgtgaagctc aatagagagg
     8581 atcttctcag aaagcagagg accttcgata acggatctat ccctcatcag atccacctcg
     8641 gagagttgca cgctatcctt agaaggcaag aggatttcta cccattcctc aaggataaca
     8701 gggaaaagat tgagaagatt ctcaccttca gaatccctta ctacgtggga cctctcgcta
     8761 gaggaaactc aagattcgct tggatgacca gaaagtctga ggaaaccatc accccttgga
     8821 acttcgaaga ggtggtggat aagggtgcta gtgctcagtc tttcatcgag aggatgacca
     8881 acttcgataa gaaccttcca aacgagaagg tgctccctaa gcactctttg ctctacgagt
     8941 acttcaccgt gtacaacgag ttgaccaagg ttaagtacgt gaccgaggga atgaggaagc
     9001 ctgctttttt gtcaggtgag caaaagaagg ctatcgttga tctcttgttc aagaccaaca
     9061 gaaaggtgac cgtgaagcag ctcaaagagg attacttcaa gaaaatcgag tgcttcgatt
     9121 cagttgagat ttctggtgtt gaggataggt tcaacgcatc tctcggaacc taccacgatc
     9181 tcctcaagat cattaaggat aaggatttct tggataacga ggaaaacgag gatatcttgg
     9241 aggatatcgt tcttaccctc accctctttg aagatagaga gatgattgaa gaaaggctca
     9301 agacctacgc tcatctcttc gatgataagg tgatgaagca gttgaagaga agaagataca
     9361 ctggttgggg aaggctctca agaaagctca ttaacggaat cagggataag cagtctggaa
     9421 agacaatcct tgatttcctc aagtctgatg gattcgctaa cagaaacttc atgcagctca
     9481 tccacgatga ttctctcacc tttaaagagg atatccagaa ggctcaggtt tcaggacagg
     9541 gtgatagtct ccatgagcat atcgctaacc tcgctggatc tcctgcaatc aagaagggaa
     9601 tcctccagac tgtgaaggtt gtggatgagt tggtgaaggt gatgggaagg cataagcctg
     9661 agaacatcgt gatcgaaatg gctagagaga accagaccac tcagaaggga cagaagaact
     9721 ctagggaaag gatgaagagg atcgaggaag gtatcaaaga gcttggatct cagatcctca
     9781 aagagcaccc tgttgagaac actcagctcc agaatgagaa gctctacctc tactacctcc
     9841 agaacggaag ggatatgtat gtggatcaag agttggatat caacaggctc tctgattacg
     9901 atgttgatca tatcgtgcca cagtcattct tgaaggatga ttctatcgat aacaaggtgc
     9961 tcaccaggtc tgataagaac aggggtaaga gtgataacgt gccaagtgaa gaggttgtga
    10021 agaaaatgaa gaactattgg aggcagctcc tcaacgctaa gctcatcact cagagaaagt
    10081 tcgataactt gactaaggct gagaggggag gactctctga attggataag gcaggattca
    10141 tcaagaggca gcttgtggaa accaggcaga tcactaagca cgttgcacag atcctcgatt
    10201 ctaggatgaa caccaagtac gatgagaacg ataagttgat cagggaagtg aaggttatca
    10261 ccctcaagtc aaagctcgtg tctgatttca gaaaggattt ccaattctac aaggtgaggg
    10321 aaatcaacaa ctaccaccac gctcacgatg cttaccttaa cgctgttgtt ggaaccgctc
    10381 tcatcaagaa gtatcctaag ctcgagtcag agttcgtgta cggtgattac aaggtgtacg
    10441 atgtgaggaa gatgatcgct aagtctgagc aagagatcgg aaaggctacc gctaagtatt
    10501 tcttctactc taacatcatg aatttcttca agaccgagat taccctcgct aacggtgaga
    10561 tcagaaagag gccactcatc gagacaaacg gtgaaacagg tgagatcgtg tgggataagg
    10621 gaagggattt cgctaccgtt agaaaggtgc tctctatgcc acaggtgaac atcgttaaga
    10681 aaaccgaggt gcagaccggt ggattctcta aagagtctat cctccctaag aggaactctg
    10741 ataagctcat tgctaggaag aaggattggg accctaagaa atacggtggt ttcgattctc
    10801 ctaccgtggc ttactctgtt ctcgttgtgg ctaaggttga gaagggaaag agtaagaagc
    10861 tcaagtctgt taaggaactt ctcggaatca ctatcatgga aaggtcatct ttcgagaaga
    10921 acccaatcga tttcctcgag gctaagggat acaaagaggt taagaaggat ctcatcatca
    10981 agctcccaaa gtactcactc ttcgaactcg agaacggtag aaagaggatg ctcgcttctg
    11041 ctggtgagct tcaaaaggga aacgagcttg ctctcccatc taagtacgtt aactttcttt
    11101 acctcgcttc tcactacgag aagttgaagg gatctccaga agataacgag cagaagcaac
    11161 ttttcgttga gcagcacaag cactacttgg atgagatcat cgagcagatc tctgagttct
    11221 ctaaaagggt gatcctcgct gatgcaaacc tcgataaggt gttgtctgct tacaacaagc
    11281 acagagataa gcctatcagg gaacaggcag agaacatcat ccatctcttc acccttacca
    11341 acctcggtgc tcctgctgct ttcaagtact tcgatacaac catcgatagg aagagataca
    11401 cctctaccaa agaagtgctc gatgctaccc tcatccatca gtctatcact ggactctacg
    11461 agactaggat cgatctctca cagctcggtg gtgattcaag ggctgatcct aagaagaaga
    11521 ggaaggtttc agctggatct ggagctacta atttttctct tcttaagcaa gctggagatg
    11581 ttgaagaaaa tcctggaccc atggtgagca agggcgagga ggataacatg gccatcatca
    11641 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
    11701 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
    11761 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
    11821 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
    11881 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
    11941 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
    12001 tcccctccga cggccccgta atgcagaaga agacgatggg ctgggaggcc tcctccgagc
    12061 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
    12121 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
    12181 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
    12241 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
    12301 tgtacaagtc aggttctggt tcaggttcag gttctggatc tggaagtgga tctggatcag
    12361 cggccgcatc tgaattcaag cgtgaagagc aagcaaggaa agctaaggtg aacaatgaga
    12421 aaaagacgga aatagtgaaa ccagagagtt gtagcaatga aggagatgtc aaggatctga
    12481 aaagaaagga ctctgaggat ggaaacgagg gtgaggaaga agaagcttct tcgaaaccga
    12541 aaaagccaaa agttgctctt tctcatcttc aggacattga cgacacagaa gctgatcaag
    12601 aagaagagta actgcccccg atgagctaag ctagctatat catcaattta tgtattacac
    12661 ataatatcgc actcagtctt tcatctacgg caatgtacca gctgatataa tcagttattg
    12721 aaatatttct gaatttaaac ttgcatcaat aaatttatgt ttttgcttgg actataatac
    12781 ctgacttgtt attttatcaa taaatattta aactatattt ctttcaagat gggaattaac
    12841 atctacaaat tgccttttct tatcgaccat gtactcgacg gccgagtact actctttttt
    12901 tcttcttctt cgttcataca gttttttttt gtttatcagc ttacattttc ttgaaccgta
    12961 gctttcgttt tcttcttttt aactttccat tcggagtttt tgtatcttgt ttcatagttt
    13021 gtcccaggat tagaatgatt aggcatcgaa ccttcaagaa tttgattgaa taaaacatct
    13081 tcattcttaa gatatgaaga taatcttcaa aaggcccctg ggaatctgaa agaagagaag
    13141 caggcccatt tatatgggaa agaacaatag tatttcttat ataggcccat ttaagttgaa
    13201 aacaatcttc aaaagtccca catcgcttag ataagaaaac gaagctgagt ttatatacag
    13261 ctagagtcga agtagtgatt ggaagatcag gctagagcag ggttttagag ctagaaatag
    13321 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
    13381 ttttaacagt attcagtcga ctggtaccaa caggctctct ttttttcttc ttcttcgttc
    13441 atacagtttt tttttgttta tcagcttaca ttttcttgaa ccgtagcttt cgttttcttc
    13501 tttttaactt tccattcgga gtttttgtat cttgtttcat agtttgtccc aggattagaa
    13561 tgattaggca tcgaaccttc aagaatttga ttgaataaaa catcttcatt cttaagatat
    13621 gaagataatc ttcaaaaggc ccctgggaat ctgaaagaag agaagcaggc ccatttatat
    13681 gggaaagaac aatagtattt cttatatagg cccatttaag ttgaaaacaa tcttcaaaag
    13741 tcccacatcg cttagataag aaaacgaagc tgagtttata tacagctaga gtcgaagtag
    13801 tgattggcta cacagattgc aggatggttt tagagctaga aatagcaagt taaaataagg
    13861 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttg tataagctta
    13921 ccttacttag atcaacacat aaaagttagt aagtgaagaa ccacaacaac aacactagat
    13981 tcatcttcaa gtgtatgtag gtatagtaac atgaacaaga acagactcaa gtacaagatc
    14041 gcatacgaaa atggaaatgg caatgtcact tccacataat caaacacgaa tcctcatatc
    14101 aacaaggcct gagattctaa ctagctcata acaacttagc caatagttac ttgagactac
    14161 caaatgtatg tagaactaaa gactaaggga cagagagttc gtctaaacag gtgaatctag
    14221 tcgttgttat ctaataaaca attcagcccc aaatgcagaa cacacataga gctctctatt
    14281 gattcaaatt acgatctgat actgataacg tctagatttt tagggttaaa gcaatcaatc
    14341 acctgacgat tcaaggtggt tggatcatga cgattccaga aaacatcaag caagctctca
    14401 aagctacact ctttgggatc atactgaact ctaacaacct cgttatgtcc cgtagtgcca
    14461 gtacagacat cctcgtaact cggattgtgc acgatgccat ggctataccc aacctcggtc
    14521 ttggtcacac caggaactct ctggtaagct agctccactc cccagaaaca accggcgcca
    14581 aattgcgcga attgctgacc tgaagacgga acatcatcgt cgggtccttg ggcgattgcg
    14641 gcggaagatg ggtcagcttg ggcttgagga cgagacgcga atccgagtct gttgaaaagg
    14701 ttgttcattg gggatttgta tacggagatt ggtcgtcgag aggtttgagg gaaaggacaa
    14761 atgggtttgg ctctggagaa agagagtgcg gctttagaga gagaattgag aggtttagag
    14821 agagatgcgg cggcgatgag cggaggagag acgacgagga cctgcattat caaagcagtg
    14881 acgtggtgaa atttggaact tttaagaggc agatagattt attatttgta tccattttct
    14941 tcattgttct agaatgtcgc ggaacaaatt ttaaaactaa atcctaaatt tttctaattt
    15001 tgttgccaat agtggatatg tgggccgtat agaaggaatc tattgaaggc ccaaacccat
    15061 actgacgagc ccaaaggttc gttttgcgtt ttatgtttcg gttcgatgcc aacgccacat
    15121 tctgagctag gcaaaaaaca aacgtgtctt tgaatagact cctctcgtta acacatgcag
    15181 cggctgcatg gtgacgccat taacacgtgg cctacaattg catgatgtct ccattgacac
    15241 gtgacttctc gtctcctttc ttaatatatc taacaaacac tcctacctct tccaaaatat
    15301 atacacatct ttttgatcaa tctctcattc aaaatctcat tctctctagt aaacaagaac
    15361 aaaaaaaaca atggcggata cagctagagg aacccatcac gatatcatcg gcagagatca
    15421 gtacccgatg atgggccgag atcgagatca gtaccagatg tccggacgag gatctgacta
    15481 ctccaagtct aggcagattg ctaaagctgc aactgctgtc acagctggtg gttccctcct
    15541 tgttctctcc agccttaccc ttgttggaac tgtcatagct ttgactgttg caacacctct
    15601 gctcgttatc ttcagcccaa tccttgtccc ggctctcatc acagttgcac tcctcatcac
    15661 cggttttctt tcctctggag ggtttggcat tgccgctata accgttttct cttggattta
    15721 caagtaagca cacatttatc atcttacttc ataattttgt gcaatatgtg catgcatgtg
    15781 ttgagccagt agctttggat caattttttt ggtcgaataa caaatgtaac aataagaaat
    15841 tgcaaattct agggaacatt tggttaacta aatacgaaat ttgacctagc tagcttgaat
    15901 gtgtctgtgt atatcatcta tataggtaaa atgcttggta tgatacctat tgattgtgaa
    15961 taggtacgca acgggagagc acccacaggg atcagacaag ttggacagtg caaggatgaa
    16021 gttgggaagc aaagctcagg atctgaaaga cagagctcag tactacggac agcaacatac
    16081 tggtggggaa catgaccgtg accgtactcg tggtggccag cacactactg gctccatggt
    16141 gtctaagggc gaagagctga tcaaggaaaa tatgcgtatg aaggtggtca tggaaggttc
    16201 ggtcaacggc caccaattca aatgcacagg tgaaggagaa ggcagaccgt acgagggaac
    16261 tcaaaccatg aggatcaaag tcatcgaggg aggacccctg ccatttgcct ttgacattct
    16321 tgccacgtcg ttcatgtatg gcagccgtac ttttatcaag tacccggccg acatccctga
    16381 tttctttaaa cagtcctttc ctgagggttt tacttgggaa agagttacga gatacgaaga
    16441 tggtggagtc gtcaccgtca cgcaggacac cagccttgag gatggcgagc tcgtctacaa
    16501 cgtcaaggtc agaggggtaa actttccctc caatggtccc gtgatgcaga agaagaccaa
    16561 gggttgggag cctaatacag agatgatgta tccagcagat ggtggtctga gaggatacac
    16621 tgacatcgca ctgaaagttg atggtggtgg ccatctgcac tgcaacttcg tgacaactta
    16681 caggtcaaaa aagaccgtcg ggaacatcaa gatgcccggt gtccatgccg ttgatcaccg
    16741 cctggaaagg atcgaggaga gtgacaatga aacctacgta gtgcaacgcg aagtggcagt
    16801 tgccaaatac agcaaccttg gtggtggcat ggacgagctg tacaagtcag gtggatccta
    16861 gataaccttt accttcattc cctgtaaatt ggtaccctgc ctagagtcaa gcagatcgtt
    16921 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta
    16981 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
    17041 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
    17101 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac
    17161 tagatcgaac tactgctcag ggcttaagct aggtagttcg cgtatgcgag tggtatctgc
    17221 aggcatgcga cgtcgggccc tctagaggat ccccgggtac cgcgaattca attcggcgtt
    17281 aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt
    17341 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac
    17401 aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt
    17461 tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct
    17521 gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag
    17581 agcgttgctg cctgtgatca
//
