LOCUS       pGG-F-AtU6-26-GFP-1-G        3179 bp    DNA     circular UNA 10-FEB-2023
DEFINITION  .
ACCESSION   p01530
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8c-dgycryy"">F</a>"
                     /label="F"
     promoter        14..401
                     /vntifkey=29
                     /modified_by="thjac"
                     /modified_by="wacae"
                     /Transferred_From="AtU6-26"
                     /Transferred_Similarity="100.00%"
                     /label="AtU6-26"
     CRISPR          402..421
                     /created_by="wacae"
                     /label="GFP-1"
     Scaffold        422..504
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
                     TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
                     /annotation_group="Scaffold: 451 -> 533"
                     /Transferred_From="Scaffold"
                     /Transferred_Similarity="100.00%"
     GG              complement(505..515)
                     /created_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="G"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:84-dgycryy"">G</a>"
                     /label="G"
     primer          complement(535..555)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1035..1139
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1140..2000
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2171..2759
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3123..3144
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaact actctttttt tcttcttctt cgttcataca gttttttttt gtttatcagc
       61 ttacattttc ttgaaccgta gctttcgttt tcttcttttt aactttccat tcggagtttt
      121 tgtatcttgt ttcatagttt gtcccaggat tagaatgatt aggcatcgaa ccttcaagaa
      181 tttgattgaa taaaacatct tcattcttaa gatatgaaga taatcttcaa aaggcccctg
      241 ggaatctgaa agaagagaag caggcccatt tatatgggaa agaacaatag tatttcttat
      301 ataggcccat ttaagttgaa aacaatcttc aaaagtccca catcgcttag ataagaaaac
      361 gaagctgagt ttatatacag ctagagtcga agtagtgatt ggctgaagca ctgcacgccg
      421 tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
      481 tggcaccgag tcggtgcttt ttttgtattg agaccgaatt ctcgccctat agtgagtcgt
      541 attacctggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
      601 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      661 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
      721 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
      781 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
      841 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
      901 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
      961 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     1021 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     1081 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     1141 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     1201 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     1261 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     1321 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     1381 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     1441 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     1501 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     1561 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     1621 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     1681 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     1741 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     1801 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggctctc
     1861 gaggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1921 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1981 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     2041 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     2101 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     2161 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     2221 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2281 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     2341 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2401 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2461 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2521 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2581 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2641 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2701 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2761 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2821 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     2881 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     2941 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     3001 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     3061 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     3121 ttgtgagcgg ataacaattt cacacgagct atttaggtga cactatagaa gtgaagctt
//
