LOCUS       pEN-L4-pSMB-CAS9PTA-G7T-R1       10261 bp    DNA     circular UNA 15-FEB-2024
DEFINITION  .
ACCESSION   p00133
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     attL4           3..99
                     /label="attL4"
     promoter        106..3159
                     /modified_by="wacae"
                     /Transferred_From="pSMB"
                     /Transferred_Similarity="100.00%"
                     /label="pSMB"
     Linker          3164..3187
                     /created_by="wacae"
                     /label="Linker"
     CDS             3194..7297
                     /Transferred_From="Cas9PTA"
                     /Transferred_Similarity="100.00%"
                     /label="CAS9"
     NLS             7298..7330
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="NLS SV40 - long"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (<a
                     href=""urn:local:.:g3-giplhnp"">NLS SV40 - long</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:12/0;
                     SV40 (<a href=""urn:local:.:9e-dgycryz"">NLS SV40 -
                     short</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:12/0;
                     SV40 (<a href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /label="SV40"
     Stop_codon      7331..7333
                     /created_by="wacae"
                     /modified_by="wacae"
                     /label
     Linker          7338..7383
                     /created_by="wacae"
                     /Transferred_From="Linker I"
                     /Transferred_Similarity="100.00%"
                     /modified_by="wacae"
                     /label="Linker"
     terminator      7388..7659
                     /Transferred_From="G7T"
                     /Transferred_Similarity="100.00%"
                     /label="G7T"
     Linker          7664..7712
                     /created_by="wacae"
                     /modified_by="wacae"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /label="Linker II"
     attR1           7718..7841
                     /label="attR1"
     primer          complement(7934..7954)
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        8015..8065
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    8066..8875
                     /modified_by="wacae"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      9022..9610
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.49%"
                     /Primary_Match="99.49%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          10209..10229
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 tccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgataagc
       61 aatgcttttt tataatgcca actttgtata gaaaagttgc tacctttggt attcatcctc
      121 aatatgcaat ttatccatca acaatgaaca cttgtaatta atttaaatga aatctcttag
      181 attgtttggc taatataaat tttcataaaa tgttttatat aataaatacc cgaaaaatgg
      241 tagtatttaa tttccctcct tgttataaaa tagccaggtg attcagtaat tcacctgaac
      301 caaacggtgt acaacacata tatatatatt gtatactaaa ggtaattgcg tatcactttg
      361 aattttatac gtacacttaa ttagccataa tggggattct aaaataagat tataagtttc
      421 agaacatgtt ggtatctcta tatgattaaa cgctcattaa taaggggttt aatgcatgac
      481 gtcttcgtga aggcaacggc acatctcaat aagataatat tgggccaaca gaaacttgta
      541 agtttcataa attgttttac gaagagatta tttaccgtga gaatccaatg tttttatgtg
      601 tttcgtgtat cttacatgga atttaggaaa ttaaaagtta ccattcatat catattcgtt
      661 tttatcttta atagttttat gtaattcgtt tggggaaaaa agtgagaaga agataaattt
      721 tgaggcgaat ctcaagaaga gagagtgaag tcaaacgagg gaccttgaat tgtggatttg
      781 gtgttggacc atcacgtgct ctcctccacg gcctttcagg attttgcttc ttctaccgca
      841 cgtgccacac tcctcctcct acgtaccggt ttccattatt gcttttttct tctttgcatt
      901 ccaattcttc ttcttattct ttttgtcaaa ggctctaaag tctctatatc tatttgttcg
      961 tttagtgaac ttttttattt atatcaaatg gattatttca taaacttgta agagttatat
     1021 ttcaggaaat catatccaca gttttgaaga tcgatccccc atgccttttt atatatacaa
     1081 attcagttat gattgtttga cgcaactgca gataaatcat gtgtatacac attataggga
     1141 aacattaata gtaatcctgc tatttgcaga accttaagaa cgtctgtgaa ctgaacgtac
     1201 gttattatgt attaatattt caactttaaa gtttaaacta aaaaaaaatt agcagaagaa
     1261 aattacatga aaattgtctt acaaaatgtt aagaaaaaaa aacgctagtg gaaatgcgcc
     1321 aatattactt gcacgttcaa tcatatagta taattagctg ttcatttaaa tcccatagtt
     1381 tctctaacgt tttttttttt tttgatgaac actctaacgt tattcatata actaatatgg
     1441 taagtaagga gcaaatatgg aactttaagg attaagatca tttgtagaaa atcttatttc
     1501 taaatttcca acgaaactgc actgaaattg atgcattaaa aaagtaagtg ggagcataat
     1561 tcaaggtacg atacatcatt tttcttccca acatttgact gcaattttga gggcatagaa
     1621 gcttcaattc ctgttttgtt ttgtccttgg gatacataaa ccagatcttg tattatatta
     1681 tatttaccat gtactatata gtattcgaag ttcattctca ttaatcgatt cctacttgtt
     1741 ttcaaagcaa attagtcagc cttactatat aacagctgta atccatagtg attactgatt
     1801 agtatagtta atcctcataa tcatacttac gtcacagtag aaaataataa tattgtagct
     1861 atctaatacg taatatatac acaattctct tcttcttttt ttttgtcaaa gatagtatac
     1921 acaattcttt agatgtatat ctaggtattt atactcgatg atttgtacaa atatgtaaat
     1981 cgaagaactt gatattaatt agaaacaaaa aacgaaacat agtgtcgaag aaaaagatta
     2041 ttttcggcac aacatcatgc acaatcttca ttcgctatag accttctaag ccctagctag
     2101 gcacaacatt ggacatggaa cggtgaatct tcaaaattat ttgcagatca ccatgtaaga
     2161 gagatacata tatatagtct agataggaat gatctgatca tcttagctga tgtttatacc
     2221 aatatgaata ggtttatata tgtggcaaga aaaattattt aatggaaact gcctatacat
     2281 attaaagtat ttctcatata tttctgtgac cagctttggt ttattaaatt taatacaaaa
     2341 taatagtggc actgatccat agaagccaag cttctctatc tttctctttc cacactttct
     2401 ttctatctca aagaagaaga aagagagaga gagagagata gaaaccctaa acatcgtgcc
     2461 cttgaagcta agatatcttc agctttgtag aaattcattt tgcttacttc attgcgctct
     2521 ctctctttca ttgtcttcta cctcttgcct ctaacagaag caatactttc cggcctccaa
     2581 ggttcgtttc tttcctcatc catcttatat ctttcattgt cgtcatcatc atctgcatct
     2641 acatatatat acacacacgc actccctcac acttcctcat atatatcatg tgtacttttc
     2701 gtcttaaaca tctctctatt ttcatttctt ttctttgatt acttaagaac atgactaagt
     2761 ctctatcatt aaacctctct tgtttaagaa gcagacaaag atcgttcagt ttgcagcacc
     2821 ttcatgtttc tcttggacat tcaggtttta cttatgtggg tatatgtaat agaagattca
     2881 tcttttgttt taaaattaaa ggttaataag ttggagcctt tgttcttcgt gactatatgc
     2941 atgcatttgg taaacctagg gttttgattt ttagatctaa tataacccaa accccaatta
     3001 acaccttgag attattggat acatacttgc atggtcaaac ccattcattt ctcaatttgt
     3061 taaaacttaa aaccttcaat ataatgtgtg tttctttctt tggggttaat taatatttca
     3121 ttagggcaaa ggtcgaaaag aaagcgtcaa agtttgctta acagtattca gtcgactggt
     3181 accaacaggc tccatggata agaagtactc tatcggactc gatatcggaa ctaactctgt
     3241 gggatgggct gtgatcaccg atgagtacaa ggtgccatct aagaagttca aggttctcgg
     3301 aaacaccgat aggcactcta tcaagaaaaa ccttatcggt gctctcctct tcgattctgg
     3361 tgaaactgct gaggctacca gactcaagag aaccgctaga agaaggtaca ccagaagaaa
     3421 gaacaggatc tgctacctcc aagagatctt ctctaacgag atggctaaag tggatgattc
     3481 attcttccac aggctcgaag agtcattcct cgtggaagaa gataagaagc acgagaggca
     3541 ccctatcttc ggaaacatcg ttgatgaggt ggcataccac gagaagtacc ctactatcta
     3601 ccacctcaga aagaagctcg ttgattctac tgataaggct gatctcaggc tcatctacct
     3661 cgctctcgct cacatgatca agttcagagg acacttcctc atcgagggtg atctcaaccc
     3721 tgataactct gatgtggata agttgttcat ccagctcgtg cagacctaca accagctttt
     3781 cgaagagaac cctatcaacg cttcaggtgt ggatgctaag gctatcctct ctgctaggct
     3841 ctctaagtca agaaggcttg agaacctcat tgctcagctc cctggtgaga agaagaacgg
     3901 acttttcgga aacttgatcg ctctctctct cggactcacc cctaacttca agtctaactt
     3961 cgatctcgct gaggatgcaa agctccagct ctcaaaggat acctacgatg atgatctcga
     4021 taacctcctc gctcagatcg gagatcagta cgctgatttg ttcctcgctg ctaagaacct
     4081 ctctgatgct atcctcctca gtgatatcct cagagtgaac accgagatca ccaaggctcc
     4141 actctcagct tctatgatca agagatacga tgagcaccac caggatctca cacttctcaa
     4201 ggctcttgtt agacagcagc tcccagagaa gtacaaagag attttcttcg atcagtctaa
     4261 gaacggatac gctggttaca tcgatggtgg tgcatctcaa gaagagttct acaagttcat
     4321 caagcctatc ctcgagaaga tggatggaac cgaggaactc ctcgtgaagc tcaatagaga
     4381 ggatcttctc agaaagcaga ggaccttcga taacggatct atccctcatc agatccacct
     4441 cggagagttg cacgctatcc ttagaaggca agaggatttc tacccattcc tcaaggataa
     4501 cagggaaaag attgagaaga ttctcacctt cagaatccct tactacgtgg gacctctcgc
     4561 tagaggaaac tcaagattcg cttggatgac cagaaagtct gaggaaacca tcaccccttg
     4621 gaacttcgaa gaggtggtgg ataagggtgc tagtgctcag tctttcatcg agaggatgac
     4681 caacttcgat aagaaccttc caaacgagaa ggtgctccct aagcactctt tgctctacga
     4741 gtacttcacc gtgtacaacg agttgaccaa ggttaagtac gtgaccgagg gaatgaggaa
     4801 gcctgctttt ttgtcaggtg agcaaaagaa ggctatcgtt gatctcttgt tcaagaccaa
     4861 cagaaaggtg accgtgaagc agctcaaaga ggattacttc aagaaaatcg agtgcttcga
     4921 ttcagttgag atttctggtg ttgaggatag gttcaacgca tctctcggaa cctaccacga
     4981 tctcctcaag atcattaagg ataaggattt cttggataac gaggaaaacg aggatatctt
     5041 ggaggatatc gttcttaccc tcaccctctt tgaagataga gagatgattg aagaaaggct
     5101 caagacctac gctcatctct tcgatgataa ggtgatgaag cagttgaaga gaagaagata
     5161 cactggttgg ggaaggctct caagaaagct cattaacgga atcagggata agcagtctgg
     5221 aaagacaatc cttgatttcc tcaagtctga tggattcgct aacagaaact tcatgcagct
     5281 catccacgat gattctctca cctttaaaga ggatatccag aaggctcagg tttcaggaca
     5341 gggtgatagt ctccatgagc atatcgctaa cctcgctgga tctcctgcaa tcaagaaggg
     5401 aatcctccag actgtgaagg ttgtggatga gttggtgaag gtgatgggaa ggcataagcc
     5461 tgagaacatc gtgatcgaaa tggctagaga gaaccagacc actcagaagg gacagaagaa
     5521 ctctagggaa aggatgaaga ggatcgagga aggtatcaaa gagcttggat ctcagatcct
     5581 caaagagcac cctgttgaga acactcagct ccagaatgag aagctctacc tctactacct
     5641 ccagaacgga agggatatgt atgtggatca agagttggat atcaacaggc tctctgatta
     5701 cgatgttgat catatcgtgc cacagtcatt cttgaaggat gattctatcg ataacaaggt
     5761 gctcaccagg tctgataaga acaggggtaa gagtgataac gtgccaagtg aagaggttgt
     5821 gaagaaaatg aagaactatt ggaggcagct cctcaacgct aagctcatca ctcagagaaa
     5881 gttcgataac ttgactaagg ctgagagggg aggactctct gaattggata aggcaggatt
     5941 catcaagagg cagcttgtgg aaaccaggca gatcactaag cacgttgcac agatcctcga
     6001 ttctaggatg aacaccaagt acgatgagaa cgataagttg atcagggaag tgaaggttat
     6061 caccctcaag tcaaagctcg tgtctgattt cagaaaggat ttccaattct acaaggtgag
     6121 ggaaatcaac aactaccacc acgctcacga tgcttacctt aacgctgttg ttggaaccgc
     6181 tctcatcaag aagtatccta agctcgagtc agagttcgtg tacggtgatt acaaggtgta
     6241 cgatgtgagg aagatgatcg ctaagtctga gcaagagatc ggaaaggcta ccgctaagta
     6301 tttcttctac tctaacatca tgaatttctt caagaccgag attaccctcg ctaacggtga
     6361 gatcagaaag aggccactca tcgagacaaa cggtgaaaca ggtgagatcg tgtgggataa
     6421 gggaagggat ttcgctaccg ttagaaaggt gctctctatg ccacaggtga acatcgttaa
     6481 gaaaaccgag gtgcagaccg gtggattctc taaagagtct atcctcccta agaggaactc
     6541 tgataagctc attgctagga agaaggattg ggaccctaag aaatacggtg gtttcgattc
     6601 tcctaccgtg gcttactctg ttctcgttgt ggctaaggtt gagaagggaa agagtaagaa
     6661 gctcaagtct gttaaggaac ttctcggaat cactatcatg gaaaggtcat ctttcgagaa
     6721 gaacccaatc gatttcctcg aggctaaggg atacaaagag gttaagaagg atctcatcat
     6781 caagctccca aagtactcac tcttcgaact cgagaacggt agaaagagga tgctcgcttc
     6841 tgctggtgag cttcaaaagg gaaacgagct tgctctccca tctaagtacg ttaactttct
     6901 ttacctcgct tctcactacg agaagttgaa gggatctcca gaagataacg agcagaagca
     6961 acttttcgtt gagcagcaca agcactactt ggatgagatc atcgagcaga tctctgagtt
     7021 ctctaaaagg gtgatcctcg ctgatgcaaa cctcgataag gtgttgtctg cttacaacaa
     7081 gcacagagat aagcctatca gggaacaggc agagaacatc atccatctct tcacccttac
     7141 caacctcggt gctcctgctg ctttcaagta cttcgataca accatcgata ggaagagata
     7201 cacctctacc aaagaagtgc tcgatgctac cctcatccat cagtctatca ctggactcta
     7261 cgagactagg atcgatctct cacagctcgg tggtgattca agggctgatc ctaagaagaa
     7321 gaggaaggtt tgatcaggtg gatcctagat aacctttacc ttcattccct gtaaattggt
     7381 accctgcccc cgatgagcta agctagctat atcatcaatt tatgtattac acataatatc
     7441 gcactcagtc tttcatctac ggcaatgtac cagctgatat aatcagttat tgaaatattt
     7501 ctgaatttaa acttgcatca ataaatttat gtttttgctt ggactataat acctgacttg
     7561 ttattttatc aataaatatt taaactatat ttctttcaag atgggaatta acatctacaa
     7621 attgcctttt cttatcgacc atgtactcga cggccgagta ctataagcta ggtagtaact
     7681 agtgcgactg tctagaatcg cgtatgcgag tggtatgcaa gtttgtacaa aaaagttgaa
     7741 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
     7801 actacataat actgtaaaac acaacatatg cagtcactat gaaccaacta cttagatggt
     7861 attagtgacc tgtagaattc gagctctaga gctgcagggc ggccgcgata tcccctatag
     7921 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     7981 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     8041 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
     8101 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     8161 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     8221 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     8281 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     8341 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
     8401 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     8461 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     8521 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     8581 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     8641 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     8701 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     8761 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     8821 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     8881 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag
     8941 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     9001 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9061 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9121 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     9181 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9241 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     9301 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     9361 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga
     9421 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9481 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     9541 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9601 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9661 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     9721 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     9781 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     9841 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     9901 tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg
     9961 gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg
    10021 ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg
    10081 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt
    10141 atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc
    10201 acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc gcgttaacgc taccatggag
    10261 c
//
