LOCUS       pB-pSMB-Cas9-G7T-AtU6-GFP-1       16349 bp    DNA     circular UNA 08-FEB-2023
DEFINITION  .
ACCESSION   p00149
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     Promoter        3..277
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    278..1069
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1315..1903
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     regulatory      complement(1939..2199)
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /Alternative_Match="99.29%; Adjusted-Interval-0:30/150; <a
                     href=""urn:local:.:79-dlucaql"">bom</a>"
                     /label="pBR322 bom"
     rep_origin      2573..2767
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     regulatory      complement(2833..3906)
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     regulatory      complement(4335..4964)
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     T-DNA_border    6150..6380
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6f-dgycryw"">RB</a>"
                     /Alternative_Match="99.50%; Adjusted-Interval-0:30/0; <a
                     href=""urn:local:.:3c-djznfva"">RB</a>"
                     /label="RB"
     promoter        6415..9468
                     /modified_by="wacae"
                     /Transferred_From="pSMB"
                     /Transferred_Similarity="100.00%"
                     /label="pSMB"
     CDS             9503..13606
                     /Transferred_From="Cas9PTA"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="AtCAS9"
     NLS             13619..13639
                     /created_by="warddecaestecker"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS SV40"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (<a
                     href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /label="SV40"
     terminator      13647..13918
                     /Transferred_From="G7T"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="tG7"
     promoter        13956..14343
                     /vntifkey=29
                     /modified_by="thjac"
                     /modified_by="wacae"
                     /Transferred_From="AtU6-26"
                     /Transferred_Similarity="100.00%"
                     /label="AtU6-26"
     CRISPR          14344..14363
                     /created_by="wacae"
                     /label="GFP-1"
     Scaffold        14364..14446
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
                     TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
                     /annotation_group="Scaffold: 451 -> 533"
                     /Transferred_From="Scaffold"
                     /Transferred_Similarity="100.00%"
     Linker          14452..14500
                     /created_by="wacae"
                     /modified_by="wacae"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /label="Linker II"
     terminator      14574..14813
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="35ST"
                     /Transferred_Similarity="95.00%"
                     /Primary_Match="95.00%; Adjusted-Interval-0:0/0; t35S (<a
                     href=""urn:local:.:6d-dgycryw"">35ST</a>)"
                     /label="t35S"
     Plant_Selection 14863..15996
                     /created_by="mamee"
                     /label="Basta"
     promoter        14863..15150
                     /created_by="thjac"
                     /modified_by="wacae"
                     /Transferred_From="pNOS"
                     /Transferred_Similarity="100.00%"
                     /label="pNOS"
     CDS             15171..15722
                     /%_Identity=100
                     /Motif="ATGAGCCCAGAACGACGCCCGGCCGACATCCGCCGTGCCACCGAGGCGGA
                     CATGCCGGCGGTCTGCACCATCGTCAACCACTACATCGAGACAAGCACGGTCAACTTC
                     CGTACCGAGCCGCAGGAACCGCAGGAGTGGACGGACGACCTCGTCCGTCTGCGGGAGC
                     GCTATCCCTGGCTCGTCGCCGAGGTGGACGGCGAGGTCGCCGGCATCGCCTACGCGGG
                     TCCCTGGAAGGCACGCAACGCCTACGACTGGACGGCCGAGTCGACCGTGTACGTCTCC
                     CCCCGCCACCAGCGGACGGGACTGGGCTCCACGCTCTACACCCACCTGCTGAAGTCCC
                     TGGAGGCACAGGGCTTCAAGAGCGTGGTCGCTGTCATCGGGCTGCCCAACGACCCGAG
                     CGTGCGCATGCACGAGGCGCTCGGATATGCCCCCCGCGGCATGCTGCGGGCGGCCGGC
                     TTCAAGCACGGGAACTGGCATGACGTGGGTTTCTGGCAGCTGGACTTCAGCCTGCCGG
                     TGCCGCCCCGTCCGGTCCTGCCCGTCACCGAAATCTGA"
                     /annotation_group="PAT: 8,732 -> 9,283"
                     /modified_by="wacae"
                     /Transferred_From="PAT"
                     /Transferred_Similarity="100.00%"
                     /label="PAT"
     terminator      15729..15996
                     /Transferred_From="NOST"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="tNOS"
     T-DNA_border    16017..16349
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
ORIGIN      
        1 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
       61 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
      121 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
      181 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
      241 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga
      301 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
      361 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
      421 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
      481 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
      541 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
      601 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
      661 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
      721 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
      781 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
      841 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
      901 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
      961 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     1021 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag
     1081 aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta
     1141 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg
     1201 tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg
     1261 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1321 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1381 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1441 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     1501 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1561 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1621 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1681 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     1741 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     1801 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     1861 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     1921 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     1981 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2041 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     2101 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     2161 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     2221 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     2281 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     2341 tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag
     2401 tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt
     2461 tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga
     2521 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca
     2581 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc
     2641 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg
     2701 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct
     2761 atccacagga aagagagctt ttcgaccttt ttcccctgct agggcaattt gccctagcat
     2821 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat
     2881 gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt
     2941 tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact
     3001 gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc
     3061 caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt
     3121 cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat
     3181 ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc
     3241 ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc
     3301 aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc
     3361 atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg
     3421 cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat
     3481 cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac
     3541 gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag
     3601 acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat
     3661 cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc
     3721 ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc
     3781 accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt
     3841 ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc
     3901 gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc
     3961 cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg
     4021 gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc
     4081 atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt
     4141 aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa
     4201 ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt
     4261 gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc
     4321 tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg
     4381 cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct
     4441 gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc
     4501 gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc
     4561 gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg
     4621 gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg
     4681 atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc
     4741 cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc
     4801 ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact
     4861 aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc
     4921 ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta
     4981 tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact
     5041 caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg
     5101 gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg
     5161 agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt
     5221 cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg
     5281 gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc
     5341 accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag
     5401 ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc
     5461 gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt
     5521 cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg
     5581 ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt
     5641 gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc
     5701 aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac
     5761 gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc
     5821 ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt
     5881 ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct
     5941 cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa
     6001 acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga
     6061 cttgtgcgac atgtcgtttt cagaagaggg ctgcactgaa cgtcagaagc cgactgcact
     6121 atagcagcgg aggggttgga tcaaagtact ttaaagtact ttaaagtact ttaaagtact
     6181 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
     6241 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
     6301 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
     6361 atccaagctc aagctaagct tgagctccaa ctttgtatag aaaagttgct acctttggta
     6421 ttcatcctca atatgcaatt tatccatcaa caatgaacac ttgtaattaa tttaaatgaa
     6481 atctcttaga ttgtttggct aatataaatt ttcataaaat gttttatata ataaataccc
     6541 gaaaaatggt agtatttaat ttccctcctt gttataaaat agccaggtga ttcagtaatt
     6601 cacctgaacc aaacggtgta caacacatat atatatattg tatactaaag gtaattgcgt
     6661 atcactttga attttatacg tacacttaat tagccataat ggggattcta aaataagatt
     6721 ataagtttca gaacatgttg gtatctctat atgattaaac gctcattaat aaggggttta
     6781 atgcatgacg tcttcgtgaa ggcaacggca catctcaata agataatatt gggccaacag
     6841 aaacttgtaa gtttcataaa ttgttttacg aagagattat ttaccgtgag aatccaatgt
     6901 ttttatgtgt ttcgtgtatc ttacatggaa tttaggaaat taaaagttac cattcatatc
     6961 atattcgttt ttatctttaa tagttttatg taattcgttt ggggaaaaaa gtgagaagaa
     7021 gataaatttt gaggcgaatc tcaagaagag agagtgaagt caaacgaggg accttgaatt
     7081 gtggatttgg tgttggacca tcacgtgctc tcctccacgg cctttcagga ttttgcttct
     7141 tctaccgcac gtgccacact cctcctccta cgtaccggtt tccattattg cttttttctt
     7201 ctttgcattc caattcttct tcttattctt tttgtcaaag gctctaaagt ctctatatct
     7261 atttgttcgt ttagtgaact tttttattta tatcaaatgg attatttcat aaacttgtaa
     7321 gagttatatt tcaggaaatc atatccacag ttttgaagat cgatccccca tgccttttta
     7381 tatatacaaa ttcagttatg attgtttgac gcaactgcag ataaatcatg tgtatacaca
     7441 ttatagggaa acattaatag taatcctgct atttgcagaa ccttaagaac gtctgtgaac
     7501 tgaacgtacg ttattatgta ttaatatttc aactttaaag tttaaactaa aaaaaaatta
     7561 gcagaagaaa attacatgaa aattgtctta caaaatgtta agaaaaaaaa acgctagtgg
     7621 aaatgcgcca atattacttg cacgttcaat catatagtat aattagctgt tcatttaaat
     7681 cccatagttt ctctaacgtt tttttttttt ttgatgaaca ctctaacgtt attcatataa
     7741 ctaatatggt aagtaaggag caaatatgga actttaagga ttaagatcat ttgtagaaaa
     7801 tcttatttct aaatttccaa cgaaactgca ctgaaattga tgcattaaaa aagtaagtgg
     7861 gagcataatt caaggtacga tacatcattt ttcttcccaa catttgactg caattttgag
     7921 ggcatagaag cttcaattcc tgttttgttt tgtccttggg atacataaac cagatcttgt
     7981 attatattat atttaccatg tactatatag tattcgaagt tcattctcat taatcgattc
     8041 ctacttgttt tcaaagcaaa ttagtcagcc ttactatata acagctgtaa tccatagtga
     8101 ttactgatta gtatagttaa tcctcataat catacttacg tcacagtaga aaataataat
     8161 attgtagcta tctaatacgt aatatataca caattctctt cttctttttt tttgtcaaag
     8221 atagtataca caattcttta gatgtatatc taggtattta tactcgatga tttgtacaaa
     8281 tatgtaaatc gaagaacttg atattaatta gaaacaaaaa acgaaacata gtgtcgaaga
     8341 aaaagattat tttcggcaca acatcatgca caatcttcat tcgctataga ccttctaagc
     8401 cctagctagg cacaacattg gacatggaac ggtgaatctt caaaattatt tgcagatcac
     8461 catgtaagag agatacatat atatagtcta gataggaatg atctgatcat cttagctgat
     8521 gtttatacca atatgaatag gtttatatat gtggcaagaa aaattattta atggaaactg
     8581 cctatacata ttaaagtatt tctcatatat ttctgtgacc agctttggtt tattaaattt
     8641 aatacaaaat aatagtggca ctgatccata gaagccaagc ttctctatct ttctctttcc
     8701 acactttctt tctatctcaa agaagaagaa agagagagag agagagatag aaaccctaaa
     8761 catcgtgccc ttgaagctaa gatatcttca gctttgtaga aattcatttt gcttacttca
     8821 ttgcgctctc tctctttcat tgtcttctac ctcttgcctc taacagaagc aatactttcc
     8881 ggcctccaag gttcgtttct ttcctcatcc atcttatatc tttcattgtc gtcatcatca
     8941 tctgcatcta catatatata cacacacgca ctccctcaca cttcctcata tatatcatgt
     9001 gtacttttcg tcttaaacat ctctctattt tcatttcttt tctttgatta cttaagaaca
     9061 tgactaagtc tctatcatta aacctctctt gtttaagaag cagacaaaga tcgttcagtt
     9121 tgcagcacct tcatgtttct cttggacatt caggttttac ttatgtgggt atatgtaata
     9181 gaagattcat cttttgtttt aaaattaaag gttaataagt tggagccttt gttcttcgtg
     9241 actatatgca tgcatttggt aaacctaggg ttttgatttt tagatctaat ataacccaaa
     9301 ccccaattaa caccttgaga ttattggata catacttgca tggtcaaacc cattcatttc
     9361 tcaatttgtt aaaacttaaa accttcaata taatgtgtgt ttctttcttt ggggttaatt
     9421 aatatttcat tagggcaaag gtcgaaaaga aagcgtcaaa gtttgcttaa cagtattcag
     9481 tcgactggta ccaacaggct ccatggataa gaagtactct atcggactcg atatcggaac
     9541 taactctgtg ggatgggctg tgatcaccga tgagtacaag gtgccatcta agaagttcaa
     9601 ggttctcgga aacaccgata ggcactctat caagaaaaac cttatcggtg ctctcctctt
     9661 cgattctggt gaaactgctg aggctaccag actcaagaga accgctagaa gaaggtacac
     9721 cagaagaaag aacaggatct gctacctcca agagatcttc tctaacgaga tggctaaagt
     9781 ggatgattca ttcttccaca ggctcgaaga gtcattcctc gtggaagaag ataagaagca
     9841 cgagaggcac cctatcttcg gaaacatcgt tgatgaggtg gcataccacg agaagtaccc
     9901 tactatctac cacctcagaa agaagctcgt tgattctact gataaggctg atctcaggct
     9961 catctacctc gctctcgctc acatgatcaa gttcagagga cacttcctca tcgagggtga
    10021 tctcaaccct gataactctg atgtggataa gttgttcatc cagctcgtgc agacctacaa
    10081 ccagcttttc gaagagaacc ctatcaacgc ttcaggtgtg gatgctaagg ctatcctctc
    10141 tgctaggctc tctaagtcaa gaaggcttga gaacctcatt gctcagctcc ctggtgagaa
    10201 gaagaacgga cttttcggaa acttgatcgc tctctctctc ggactcaccc ctaacttcaa
    10261 gtctaacttc gatctcgctg aggatgcaaa gctccagctc tcaaaggata cctacgatga
    10321 tgatctcgat aacctcctcg ctcagatcgg agatcagtac gctgatttgt tcctcgctgc
    10381 taagaacctc tctgatgcta tcctcctcag tgatatcctc agagtgaaca ccgagatcac
    10441 caaggctcca ctctcagctt ctatgatcaa gagatacgat gagcaccacc aggatctcac
    10501 acttctcaag gctcttgtta gacagcagct cccagagaag tacaaagaga ttttcttcga
    10561 tcagtctaag aacggatacg ctggttacat cgatggtggt gcatctcaag aagagttcta
    10621 caagttcatc aagcctatcc tcgagaagat ggatggaacc gaggaactcc tcgtgaagct
    10681 caatagagag gatcttctca gaaagcagag gaccttcgat aacggatcta tccctcatca
    10741 gatccacctc ggagagttgc acgctatcct tagaaggcaa gaggatttct acccattcct
    10801 caaggataac agggaaaaga ttgagaagat tctcaccttc agaatccctt actacgtggg
    10861 acctctcgct agaggaaact caagattcgc ttggatgacc agaaagtctg aggaaaccat
    10921 caccccttgg aacttcgaag aggtggtgga taagggtgct agtgctcagt ctttcatcga
    10981 gaggatgacc aacttcgata agaaccttcc aaacgagaag gtgctcccta agcactcttt
    11041 gctctacgag tacttcaccg tgtacaacga gttgaccaag gttaagtacg tgaccgaggg
    11101 aatgaggaag cctgcttttt tgtcaggtga gcaaaagaag gctatcgttg atctcttgtt
    11161 caagaccaac agaaaggtga ccgtgaagca gctcaaagag gattacttca agaaaatcga
    11221 gtgcttcgat tcagttgaga tttctggtgt tgaggatagg ttcaacgcat ctctcggaac
    11281 ctaccacgat ctcctcaaga tcattaagga taaggatttc ttggataacg aggaaaacga
    11341 ggatatcttg gaggatatcg ttcttaccct caccctcttt gaagatagag agatgattga
    11401 agaaaggctc aagacctacg ctcatctctt cgatgataag gtgatgaagc agttgaagag
    11461 aagaagatac actggttggg gaaggctctc aagaaagctc attaacggaa tcagggataa
    11521 gcagtctgga aagacaatcc ttgatttcct caagtctgat ggattcgcta acagaaactt
    11581 catgcagctc atccacgatg attctctcac ctttaaagag gatatccaga aggctcaggt
    11641 ttcaggacag ggtgatagtc tccatgagca tatcgctaac ctcgctggat ctcctgcaat
    11701 caagaaggga atcctccaga ctgtgaaggt tgtggatgag ttggtgaagg tgatgggaag
    11761 gcataagcct gagaacatcg tgatcgaaat ggctagagag aaccagacca ctcagaaggg
    11821 acagaagaac tctagggaaa ggatgaagag gatcgaggaa ggtatcaaag agcttggatc
    11881 tcagatcctc aaagagcacc ctgttgagaa cactcagctc cagaatgaga agctctacct
    11941 ctactacctc cagaacggaa gggatatgta tgtggatcaa gagttggata tcaacaggct
    12001 ctctgattac gatgttgatc atatcgtgcc acagtcattc ttgaaggatg attctatcga
    12061 taacaaggtg ctcaccaggt ctgataagaa caggggtaag agtgataacg tgccaagtga
    12121 agaggttgtg aagaaaatga agaactattg gaggcagctc ctcaacgcta agctcatcac
    12181 tcagagaaag ttcgataact tgactaaggc tgagagggga ggactctctg aattggataa
    12241 ggcaggattc atcaagaggc agcttgtgga aaccaggcag atcactaagc acgttgcaca
    12301 gatcctcgat tctaggatga acaccaagta cgatgagaac gataagttga tcagggaagt
    12361 gaaggttatc accctcaagt caaagctcgt gtctgatttc agaaaggatt tccaattcta
    12421 caaggtgagg gaaatcaaca actaccacca cgctcacgat gcttacctta acgctgttgt
    12481 tggaaccgct ctcatcaaga agtatcctaa gctcgagtca gagttcgtgt acggtgatta
    12541 caaggtgtac gatgtgagga agatgatcgc taagtctgag caagagatcg gaaaggctac
    12601 cgctaagtat ttcttctact ctaacatcat gaatttcttc aagaccgaga ttaccctcgc
    12661 taacggtgag atcagaaaga ggccactcat cgagacaaac ggtgaaacag gtgagatcgt
    12721 gtgggataag ggaagggatt tcgctaccgt tagaaaggtg ctctctatgc cacaggtgaa
    12781 catcgttaag aaaaccgagg tgcagaccgg tggattctct aaagagtcta tcctccctaa
    12841 gaggaactct gataagctca ttgctaggaa gaaggattgg gaccctaaga aatacggtgg
    12901 tttcgattct cctaccgtgg cttactctgt tctcgttgtg gctaaggttg agaagggaaa
    12961 gagtaagaag ctcaagtctg ttaaggaact tctcggaatc actatcatgg aaaggtcatc
    13021 tttcgagaag aacccaatcg atttcctcga ggctaaggga tacaaagagg ttaagaagga
    13081 tctcatcatc aagctcccaa agtactcact cttcgaactc gagaacggta gaaagaggat
    13141 gctcgcttct gctggtgagc ttcaaaaggg aaacgagctt gctctcccat ctaagtacgt
    13201 taactttctt tacctcgctt ctcactacga gaagttgaag ggatctccag aagataacga
    13261 gcagaagcaa cttttcgttg agcagcacaa gcactacttg gatgagatca tcgagcagat
    13321 ctctgagttc tctaaaaggg tgatcctcgc tgatgcaaac ctcgataagg tgttgtctgc
    13381 ttacaacaag cacagagata agcctatcag ggaacaggca gagaacatca tccatctctt
    13441 cacccttacc aacctcggtg ctcctgctgc tttcaagtac ttcgatacaa ccatcgatag
    13501 gaagagatac acctctacca aagaagtgct cgatgctacc ctcatccatc agtctatcac
    13561 tggactctac gagactagga tcgatctctc acagctcggt ggtgattcaa gggctgatcc
    13621 taagaagaag aggaaggttt gatcagcccc gatgagctaa gctagctata tcatcaattt
    13681 atgtattaca cataatatcg cactcagtct ttcatctacg gcaatgtacc agctgatata
    13741 atcagttatt gaaatatttc tgaatttaaa cttgcatcaa taaatttatg tttttgcttg
    13801 gactataata cctgacttgt tattttatca ataaatattt aaactatatt tctttcaaga
    13861 tgggaattaa catctacaaa ttgccttttc ttatcgacca tgtactcgac ggccgagtgt
    13921 atgcaagttt gtacaaaaaa gcaggcttaa cctctctttt tttcttcttc ttcgttcata
    13981 cagttttttt ttgtttatca gcttacattt tcttgaaccg tagctttcgt tttcttcttt
    14041 ttaactttcc attcggagtt tttgtatctt gtttcatagt ttgtcccagg attagaatga
    14101 ttaggcatcg aaccttcaag aatttgattg aataaaacat cttcattctt aagatatgaa
    14161 gataatcttc aaaaggcccc tgggaatctg aaagaagaga agcaggccca tttatatggg
    14221 aaagaacaat agtatttctt atataggccc atttaagttg aaaacaatct tcaaaagtcc
    14281 cacatcgctt agataagaaa acgaagctga gtttatatac agctagagtc gaagtagtga
    14341 ttggctgaag cactgcacgc cgtgttttag agctagaaat agcaagttaa aataaggcta
    14401 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttaaac ataagctagg
    14461 tagtaactag tgcgactgtc tagaatcgcg tatgcgagtg gtattaccca gctttcttgt
    14521 acaaagtggt gatatcccgc ggccatggcg gccgggagca tgcgacgtcg atctaactga
    14581 ctagccgcgg ccatgctaga gtccgcaaaa atcaccagtc tctctctaca aatctatctc
    14641 tctctatttt tctccagaat aatgtgtgag tagttcccag ataagggaat tagggttctt
    14701 atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta
    14761 aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtga cctgcaggca
    14821 tgcgacgtcg ggccctctag aggatccccg ggtaccgcga attatcgatc atgagcggag
    14881 aattaaggga gtcacgttat gacccccgcc gatgacgcgg gacaagccgt tttacgtttg
    14941 gaactgacag aaccgcaacg ttgaaggagc cactgagccg cgggtttctg gagtttaatg
    15001 agctaagcac atacgtcaga aaccattatt gcgcgttcaa aagtcgccta aggtcactat
    15061 cagctagcaa atatttcttg tcaaaaatgc tccactgacg ttccataaat tcccctcggt
    15121 atccaattag agtctcatat tcactctcaa ctcgatcgag gggatctacc atgagcccag
    15181 aacgacgccc ggccgacatc cgccgtgcca ccgaggcgga catgccggcg gtctgcacca
    15241 tcgtcaacca ctacatcgag acaagcacgg tcaacttccg taccgagccg caggaaccgc
    15301 aggagtggac ggacgacctc gtccgtctgc gggagcgcta tccctggctc gtcgccgagg
    15361 tggacggcga ggtcgccggc atcgcctacg cgggtccctg gaaggcacgc aacgcctacg
    15421 actggacggc cgagtcgacc gtgtacgtct ccccccgcca ccagcggacg ggactgggct
    15481 ccacgctcta cacccacctg ctgaagtccc tggaggcaca gggcttcaag agcgtggtcg
    15541 ctgtcatcgg gctgcccaac gacccgagcg tgcgcatgca cgaggcgctc ggatatgccc
    15601 cccgcggcat gctgcgggcg gccggcttca agcacgggaa ctggcatgac gtgggtttct
    15661 ggcagctgga cttcagcctg ccggtgccgc cccgtccggt cctgcccgtc accgaaatct
    15721 gatgacccct agagtcaagc agatcgttca aacatttggc aataaagttt cttaagattg
    15781 aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat
    15841 gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc
    15901 ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa
    15961 ttatcgcgcg cggtgtcatc tatgttacta gatcgaccgg catgcaagct gataattcaa
    16021 ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt
    16081 caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca
    16141 gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg
    16201 gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc
    16261 aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa
    16321 cgatgacaga gcgttgctgc ctgtgatca
//
