LOCUS       pGG-F-AtU6-26-AarI-AarI-G        3183 bp    DNA     circular UNA 15-MAR-2023
DEFINITION  .
ACCESSION   p00328
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8c-dgycryy"">F</a>"
                     /label="F"
     promoter        14..401
                     /vntifkey=29
                     /modified_by="thjac"
                     /modified_by="wacae"
                     /Transferred_From="AtU6-26"
                     /Transferred_Similarity="100.00%"
                     /label="AtU6-26"
     Scaffold        426..508
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
                     TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
                     /annotation_group="Scaffold: 451 -> 533"
                     /Transferred_From="Scaffold"
                     /Transferred_Similarity="100.00%"
     GG              complement(509..519)
                     /created_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="G"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:84-dgycryy"">G</a>"
                     /label="G"
     primer          complement(539..559)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1039..1143
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1144..2004
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2175..2763
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3127..3148
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaact actctttttt tcttcttctt cgttcataca gttttttttt gtttatcagc
       61 ttacattttc ttgaaccgta gctttcgttt tcttcttttt aactttccat tcggagtttt
      121 tgtatcttgt ttcatagttt gtcccaggat tagaatgatt aggcatcgaa ccttcaagaa
      181 tttgattgaa taaaacatct tcattcttaa gatatgaaga taatcttcaa aaggcccctg
      241 ggaatctgaa agaagagaag caggcccatt tatatgggaa agaacaatag tatttcttat
      301 ataggcccat ttaagttgaa aacaatcttc aaaagtccca catcgcttag ataagaaaac
      361 gaagctgagt ttatatacag ctagagtcga agtagtgatt gggatgcagg tggacacctg
      421 cagctgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
      481 aaagtggcac cgagtcggtg ctttttttgt attgagaccg aattctcgcc ctatagtgag
      541 tcgtattacc tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      601 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      661 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      721 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      781 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
      841 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
      901 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
      961 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     1021 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     1081 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1141 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     1201 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1261 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1321 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1381 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1441 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1501 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1561 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1621 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1681 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1741 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1801 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggc
     1861 tctcgaggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1921 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1981 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     2041 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     2101 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2161 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2221 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2281 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2341 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2401 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2461 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2521 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2581 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2641 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2701 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2761 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2821 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2881 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     2941 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     3001 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     3061 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     3121 ggaattgtga gcggataaca atttcacacg agctatttag gtgacactat agaagtgaag
     3181 ctt
//
