LOCUS       pGG-C-Cas9-SV40-D        6825 bp    DNA     circular UNA 08-FEB-2023
DEFINITION  .
ACCESSION   p00424
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:88-dgycryy"">C</a>"
                     /label="C"
     CDS             14..4117
                     /Transferred_From="Cas9PTA"
                     /Transferred_Similarity="100.00%"
                     /label="CAS9"
     NLS             4130..4150
                     /created_by="warddecaestecker"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS SV40"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (<a
                     href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /label="SV40"
     GG              complement(4151..4161)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:81-dgycryx"">D</a>"
                     /label="D"
     primer          complement(4181..4201)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        4681..4785
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    4786..5646
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      5817..6405
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          6769..6790
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tcgatggata agaagtactc tatcggactc gatatcggaa ctaactctgt
       61 gggatgggct gtgatcaccg atgagtacaa ggtgccatct aagaagttca aggttctcgg
      121 aaacaccgat aggcactcta tcaagaaaaa ccttatcggt gctctcctct tcgattctgg
      181 tgaaactgct gaggctacca gactcaagag aaccgctaga agaaggtaca ccagaagaaa
      241 gaacaggatc tgctacctcc aagagatctt ctctaacgag atggctaaag tggatgattc
      301 attcttccac aggctcgaag agtcattcct cgtggaagaa gataagaagc acgagaggca
      361 ccctatcttc ggaaacatcg ttgatgaggt ggcataccac gagaagtacc ctactatcta
      421 ccacctcaga aagaagctcg ttgattctac tgataaggct gatctcaggc tcatctacct
      481 cgctctcgct cacatgatca agttcagagg acacttcctc atcgagggtg atctcaaccc
      541 tgataactct gatgtggata agttgttcat ccagctcgtg cagacctaca accagctttt
      601 cgaagagaac cctatcaacg cttcaggtgt ggatgctaag gctatcctct ctgctaggct
      661 ctctaagtca agaaggcttg agaacctcat tgctcagctc cctggtgaga agaagaacgg
      721 acttttcgga aacttgatcg ctctctctct cggactcacc cctaacttca agtctaactt
      781 cgatctcgct gaggatgcaa agctccagct ctcaaaggat acctacgatg atgatctcga
      841 taacctcctc gctcagatcg gagatcagta cgctgatttg ttcctcgctg ctaagaacct
      901 ctctgatgct atcctcctca gtgatatcct cagagtgaac accgagatca ccaaggctcc
      961 actctcagct tctatgatca agagatacga tgagcaccac caggatctca cacttctcaa
     1021 ggctcttgtt agacagcagc tcccagagaa gtacaaagag attttcttcg atcagtctaa
     1081 gaacggatac gctggttaca tcgatggtgg tgcatctcaa gaagagttct acaagttcat
     1141 caagcctatc ctcgagaaga tggatggaac cgaggaactc ctcgtgaagc tcaatagaga
     1201 ggatcttctc agaaagcaga ggaccttcga taacggatct atccctcatc agatccacct
     1261 cggagagttg cacgctatcc ttagaaggca agaggatttc tacccattcc tcaaggataa
     1321 cagggaaaag attgagaaga ttctcacctt cagaatccct tactacgtgg gacctctcgc
     1381 tagaggaaac tcaagattcg cttggatgac cagaaagtct gaggaaacca tcaccccttg
     1441 gaacttcgaa gaggtggtgg ataagggtgc tagtgctcag tctttcatcg agaggatgac
     1501 caacttcgat aagaaccttc caaacgagaa ggtgctccct aagcactctt tgctctacga
     1561 gtacttcacc gtgtacaacg agttgaccaa ggttaagtac gtgaccgagg gaatgaggaa
     1621 gcctgctttt ttgtcaggtg agcaaaagaa ggctatcgtt gatctcttgt tcaagaccaa
     1681 cagaaaggtg accgtgaagc agctcaaaga ggattacttc aagaaaatcg agtgcttcga
     1741 ttcagttgag atttctggtg ttgaggatag gttcaacgca tctctcggaa cctaccacga
     1801 tctcctcaag atcattaagg ataaggattt cttggataac gaggaaaacg aggatatctt
     1861 ggaggatatc gttcttaccc tcaccctctt tgaagataga gagatgattg aagaaaggct
     1921 caagacctac gctcatctct tcgatgataa ggtgatgaag cagttgaaga gaagaagata
     1981 cactggttgg ggaaggctct caagaaagct cattaacgga atcagggata agcagtctgg
     2041 aaagacaatc cttgatttcc tcaagtctga tggattcgct aacagaaact tcatgcagct
     2101 catccacgat gattctctca cctttaaaga ggatatccag aaggctcagg tttcaggaca
     2161 gggtgatagt ctccatgagc atatcgctaa cctcgctgga tctcctgcaa tcaagaaggg
     2221 aatcctccag actgtgaagg ttgtggatga gttggtgaag gtgatgggaa ggcataagcc
     2281 tgagaacatc gtgatcgaaa tggctagaga gaaccagacc actcagaagg gacagaagaa
     2341 ctctagggaa aggatgaaga ggatcgagga aggtatcaaa gagcttggat ctcagatcct
     2401 caaagagcac cctgttgaga acactcagct ccagaatgag aagctctacc tctactacct
     2461 ccagaacgga agggatatgt atgtggatca agagttggat atcaacaggc tctctgatta
     2521 cgatgttgat catatcgtgc cacagtcatt cttgaaggat gattctatcg ataacaaggt
     2581 gctcaccagg tctgataaga acaggggtaa gagtgataac gtgccaagtg aagaggttgt
     2641 gaagaaaatg aagaactatt ggaggcagct cctcaacgct aagctcatca ctcagagaaa
     2701 gttcgataac ttgactaagg ctgagagggg aggactctct gaattggata aggcaggatt
     2761 catcaagagg cagcttgtgg aaaccaggca gatcactaag cacgttgcac agatcctcga
     2821 ttctaggatg aacaccaagt acgatgagaa cgataagttg atcagggaag tgaaggttat
     2881 caccctcaag tcaaagctcg tgtctgattt cagaaaggat ttccaattct acaaggtgag
     2941 ggaaatcaac aactaccacc acgctcacga tgcttacctt aacgctgttg ttggaaccgc
     3001 tctcatcaag aagtatccta agctcgagtc agagttcgtg tacggtgatt acaaggtgta
     3061 cgatgtgagg aagatgatcg ctaagtctga gcaagagatc ggaaaggcta ccgctaagta
     3121 tttcttctac tctaacatca tgaatttctt caagaccgag attaccctcg ctaacggtga
     3181 gatcagaaag aggccactca tcgagacaaa cggtgaaaca ggtgagatcg tgtgggataa
     3241 gggaagggat ttcgctaccg ttagaaaggt gctctctatg ccacaggtga acatcgttaa
     3301 gaaaaccgag gtgcagaccg gtggattctc taaagagtct atcctcccta agaggaactc
     3361 tgataagctc attgctagga agaaggattg ggaccctaag aaatacggtg gtttcgattc
     3421 tcctaccgtg gcttactctg ttctcgttgt ggctaaggtt gagaagggaa agagtaagaa
     3481 gctcaagtct gttaaggaac ttctcggaat cactatcatg gaaaggtcat ctttcgagaa
     3541 gaacccaatc gatttcctcg aggctaaggg atacaaagag gttaagaagg atctcatcat
     3601 caagctccca aagtactcac tcttcgaact cgagaacggt agaaagagga tgctcgcttc
     3661 tgctggtgag cttcaaaagg gaaacgagct tgctctccca tctaagtacg ttaactttct
     3721 ttacctcgct tctcactacg agaagttgaa gggatctcca gaagataacg agcagaagca
     3781 acttttcgtt gagcagcaca agcactactt ggatgagatc atcgagcaga tctctgagtt
     3841 ctctaaaagg gtgatcctcg ctgatgcaaa cctcgataag gtgttgtctg cttacaacaa
     3901 gcacagagat aagcctatca gggaacaggc agagaacatc atccatctct tcacccttac
     3961 caacctcggt gctcctgctg ctttcaagta cttcgataca accatcgata ggaagagata
     4021 cacctctacc aaagaagtgc tcgatgctac cctcatccat cagtctatca ctggactcta
     4081 cgagactagg atcgatctct cacagctcgg tggtgattca agggctgatc ctaagaagaa
     4141 gaggaaggtt tcagtgagac cgaattctcg ccctatagtg agtcgtatta cctggccgtc
     4201 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     4261 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     4321 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
     4381 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     4441 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     4501 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     4561 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     4621 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     4681 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4741 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4801 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4861 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4921 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4981 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5041 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5101 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5161 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5221 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5281 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5341 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5401 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5461 ggctggttta ttgctgataa atctggagcc ggtgagcgtg gctctcgagg tatcattgca
     5521 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5581 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5641 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5701 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5761 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5821 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5881 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5941 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6001 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6061 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6121 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6181 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6241 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6301 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6361 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6421 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6481 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     6541 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     6601 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     6661 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     6721 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     6781 caatttcaca cgagctattt aggtgacact atagaagtga agctt
//
