LOCUS       pGG-C-mRuby3-D          3399 bp    DNA     circular SYN 23-FEB-2023
DEFINITION  .
ACCESSION   p00238
KEYWORDS    .
SOURCE      Cloning vector pGGC000 (unknown)
  ORGANISM  Cloning vector pGGC000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:88-dgycryy"">C</a>"
                     /label="C"
     CDS             14..724
                     /note="added by Rabuo"
                     /modified_by="wacae"
                     /Transferred_From="mRuby3"
                     /Transferred_Similarity="100.00%"
                     /label="mRuby3"
     Source          join(<725..1159,1160..>7)
                     /organism="Cloning vector pGGC000"
                     /mol_type="other DNA"
                     /db_xref="taxon:1439662"
     GG              complement(725..735)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:81-dgycryx"">D</a>"
                     /label="D"
     primer          complement(755..775)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1255..1359
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1360..2220
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2391..2979
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3343..3364
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatggtgt ctaagggcga agagctgatc aaggaaaata tgcgtatgaa
       61 ggtggtcatg gaaggttcgg tcaacggcca ccaattcaaa tgcacaggtg aaggagaagg
      121 cagaccgtac gagggaactc aaaccatgag gatcaaagtc atcgagggag gacccctgcc
      181 atttgccttt gacattcttg ccacgtcgtt catgtatggc agccgtactt ttatcaagta
      241 cccggccgac atccctgatt tctttaaaca gtcctttcct gagggtttta cttgggaaag
      301 agttacgaga tacgaagatg gtggagtcgt caccgtcacg caggacacca gccttgagga
      361 tggcgagctc gtctacaacg tcaaggtcag aggggtaaac tttccctcca atggtcccgt
      421 gatgcagaag aagaccaagg gttgggagcc taatacagag atgatgtatc cagcagatgg
      481 tggtctgaga ggatacactg acatcgcact gaaagttgat ggtggtggcc atctgcactg
      541 caacttcgtg acaacttaca ggtcaaaaaa gaccgtcggg aacatcaaga tgcccggtgt
      601 ccatgccgtt gatcaccgcc tggaaaggat cgaggagagt gacaatgaaa cctacgtagt
      661 gcaacgcgaa gtggcagttg ccaaatacag caaccttggt ggtggcatgg acgagctgta
      721 caagtcagtg agaccgaatt ctcgccctat agtgagtcgt attacctggc cgtcgtttta
      781 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
      841 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
      901 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
      961 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     1021 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     1081 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     1141 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     1201 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     1261 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     1321 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     1381 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     1441 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     1501 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     1561 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     1621 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     1681 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     1741 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     1801 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     1861 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     1921 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     1981 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     2041 tttattgctg ataaatctgg agccggtgag cgtggctctc gaggtatcat tgcagcactg
     2101 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     2161 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     2221 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     2281 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     2341 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2401 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2461 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2521 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2581 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2641 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2701 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2761 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2821 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2881 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2941 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3001 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3061 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3121 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     3181 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     3241 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     3301 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     3361 cacacgagct atttaggtga cactatagaa gtgaagctt
//
