LOCUS       pGG-A-pFAMA-B           5100 bp    DNA     circular UNA 10-FEB-2023
DEFINITION  .
ACCESSION   p00426
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8a-dgycryy"">A</a>"
                     /label="A"
     promoter        12..2425
                     /created_by="User"
                     /Transferred_From="pFAMA"
                     /Transferred_Similarity="100.00%"
                     /label="pFAMA"
     GG              complement(2426..2436)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7z-dgycryx"">B</a>"
                     /label="B"
     primer          complement(2456..2476)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        2956..3060
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    3061..3921
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      4092..4680
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          5044..5065
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc tatcactaag tggttttact agtgaatttt tagtgttaaa tttactactc
       61 aaagaaaaat ttattgatat gagtgggaaa aaaaagtcca aaacaaaagt atgtatatga
      121 tatagatata ataaatatgt gtgtttactt ggtttgaact gcacttgtat attgacaata
      181 aatttatggt ataagaatgc aaatttttat gttttttact tttttttatg ataaaaaaga
      241 atgtaaatta ttcttttgga attaaaaaag aattgagtat atcaatcagt ctcttcgctc
      301 gctatcattg aagtttgatc ttaaaatcta tatatcttac agtatttcga tgcttgtggc
      361 ctgtgggtgt tatgtatcgt aatagttgtt ttgttggctc aattgggaat ggttgcagca
      421 agtatttttt ttggttgatg cttcgatgca agctctttca tgtttctttt tttcttttca
      481 acatagtatt tgtgtaatat gcacctacta aaatactcct atggatccgc gactcttatt
      541 cccgaaaaag ttcactggat tgatcaacgt gaatttttac tccacttttt gttaaaagtt
      601 aaaacgtaac catccacacc agtgtctttt tctttaaatg tatatgtctc cacgtacaca
      661 cacaaatagt ataggtcatg ttcgtgaaac taatttcata tatttgacca gaatattttt
      721 attagaaatc atattttatt gacaacacat aactaatata agtgcttgaa tttgaaatac
      781 tatagaaata aactaaaaga tgagaagcgt atagagattt gattaagttc acgaaaagcg
      841 gtggaaaaag agcggccttg gcttgtgaac tctaaaaact caaatcttat catttatggt
      901 ttgacaaaca aaaaaaaaag atagatagat agagaggtga tcagctgata cacgtgtgtt
      961 ttcacgttac ttcttttttc gtattttgtg gctcctcacc cttacgtcta acgcaaaaag
     1021 aaatagagta tttattacaa aaacaagata tgatttggaa tattcaataa tctttctttt
     1081 gagtcaatat tgaacttaat ttttgttcaa aataagctag ggagttgctc tagatccccc
     1141 atttcaaatt tgaccgtaaa atttggattg ttatgtgttt ctatgtagaa gatagagaag
     1201 aactagataa ctatatatgt aatgatattt atatatcaaa tgggatgaaa attaatattg
     1261 acacattatg tgcataatac aaagggattg attgaatgag catcttttga ttagccaatc
     1321 atgattttct gcaaaagcat ttggggagac attgagaact tccgactgca cggctttatc
     1381 ctgctacaat tggaagcttt ttttactaag cattaagtat ctctaacaat tataagaggg
     1441 caataataac atcccttttt ctttcttaca aagcaataaa aaaggacaat agtaacgaaa
     1501 attcattttt cccacgattg atatcttttg gttgtagttt ttagattttc taaaacattt
     1561 tatgtgatgg ttgatatttc tctactttta gaaactaaaa gagaatgcat agaaaattgc
     1621 gaaaaacaat gatgtctcga taattaatgg gaatatgata gtagaataaa acagccactg
     1681 caatcaaact acttactact aatcctcaat attttagatt aacttatgta gattttcatt
     1741 tatctttatt ctttagaaaa ttgttttttt tttttttgaa acatgtttac gtatcttttt
     1801 ttaagtcgtt taatttgttt ttattgggtc acgaatgtgt accattcaca ccctaattag
     1861 taattagaga aacaaatgat tacgaatata catacttaag aaatttaatg cccccttgct
     1921 ataccttgtg tacacatgta taatgtatct ctctctataa atatgggagc ttggtgatgc
     1981 ttactcattt atgacttctc aatacaaaaa gctcccctca cttttttaag ttttgtcttc
     2041 tctaatccgt cttcttctac tatcttgcat gtcttgcgtc ttttatatac atctctcgta
     2101 aaccctagca aatcatacaa ggtcagtccc ttttattttg ttaactcctt actaaacaaa
     2161 gctctaatat atttggtgtt aatcattttt tttaattgtc aaaattcaat ttgaaaactt
     2221 gttttgtaca tataaaatac atgtatatgt atatggtgaa acatgtaatc tgcctaggtt
     2281 tggttttaac ttgttctacg tgtgtttttg tttcatgtat atgtttttaa aactgactgt
     2341 gaagctcatg gattgttgtc atcaggtcaa gaagcttgac cttcattaga cttaagcagt
     2401 ttataatcaa ctaccacgaa tagcaaacat gagaccgaat tctcgcccta tagtgagtcg
     2461 tattacctgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     2521 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     2581 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     2641 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     2701 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     2761 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     2821 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     2881 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     2941 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3001 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3061 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     3121 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     3181 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     3241 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     3301 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     3361 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     3421 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     3481 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     3541 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     3601 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     3661 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     3721 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggctct
     3781 cgaggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     3841 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     3901 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     3961 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4021 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4081 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4141 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4201 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     4261 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4321 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4381 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4441 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     4501 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4561 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4621 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4681 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4741 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     4801 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     4861 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     4921 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     4981 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     5041 attgtgagcg gataacaatt tcacacgagc tatttaggtg acactataga agtgaagctt
//
